Publication Date:
1993-07-09
Description:
Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Butler, A -- Tsunoda, S -- McCobb, D P -- Wei, A -- Salkoff, L -- R01 NS24785-01/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1993 Jul 9;261(5118):221-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7687074" target="_blank"〉PubMed〈/a〉
Keywords:
Alternative Splicing
;
Amino Acid Sequence
;
Animals
;
Calcium/metabolism/*pharmacology
;
Charybdotoxin
;
DNA/genetics
;
Drosophila
;
Electric Conductivity
;
Large-Conductance Calcium-Activated Potassium Channels
;
Membrane Proteins/chemistry/*genetics
;
Mice
;
Molecular Sequence Data
;
Oocytes/metabolism
;
Peptides/pharmacology
;
Potassium/metabolism
;
Potassium Channels/chemistry/drug effects/*genetics/metabolism
;
*Potassium Channels, Calcium-Activated
;
RNA/genetics
;
RNA, Complementary
;
Scorpion Venoms/pharmacology
;
Sodium/metabolism
;
Tetraethylammonium
;
Tetraethylammonium Compounds/pharmacology
;
Transcription, Genetic
;
Xenopus
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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