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  • ELISA  (7)
  • Springer  (7)
  • Oxford University Press
  • 1990-1994  (7)
  • 1965-1969
  • 1993  (7)
Collection
Keywords
Publisher
  • Springer  (7)
  • Oxford University Press
Years
  • 1990-1994  (7)
  • 1965-1969
Year
  • 1
    Electronic Resource
    Electronic Resource
    Serological grouping of indigenous Bradyrhizobium sp. (Arachis) isolated from various soils of Thailand (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 635-640 
    Details
    ISSN: 1573-0972
    Keywords: Antigens ; antisera ; Arachis ; Bradyrhizobium ; ELISA ; peanut ; serological groups
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract ELISA and antibody adsorption tests were applied to determine the minimal somatic antigen constitution of 243 strains of Bradyrhizobium sp. (Arachis) using 12 antisera. The 243 indigenous bradyrhizobial isolates were from 15 sites in four regions of Thailand. A total of 29 serogroups were identified. Most (80%) of the isolates tested had at least one heat-stable antigen in common with strain 280A, forming a so-called 280A serocluster. At 11 of 15 sites tested, 53 to 100% of the isolates fell into one or two predominant serogroups. The serological properties of the indigenous bradyrhizobia were not related to the cropping history of the cultivated fields from which they were isolated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369570
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  • 2
    Electronic Resource
    Electronic Resource
    Mushroom worker's lung: Serologic reactions to thermophilic actinomycetes present in the air of compost tunnels (1993)
    Springer
    Mycopathologia 122 (1993), S. 21-28 
    Details
    ISSN: 1573-0832
    Keywords: Actinomycetes ; Allergy ; Compost ; ELISA ; Lung disease ; Mushroom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Vast numbers of spores of the thermophilic actinomycetesExcellospora flexuosa,Thermomonospora alba,T. curvata andT. fusca were collected from the air in fermentation tunnels during the spawning of mushroom compost, i.e. over 109 CFU m−3 of air. Five different genera of fungi, namely,Aspergillus,Aureobasidium,Cladosporium,Penicillium andScytalidium, were found at only 103 CFU m−3 of air.Agaricus bisporus, used for spawning, was absent. Sera of 10 mushroom growers affected by Mushroom Worker's Lung (MWL) were tested by a qualitative dot-ELISA for antibodies against the spores of these micro-organisms. All 10 were positive for one or more of the actinomycetesE. flexuosa,T. alba,T. curvata andT. fusca. No antibodies were found againstStreptomyces thermovulgaris,Thermoactinomyces vulgaris andT. sacchari nor against the fungiAspergillus fumigatus,Penicillium brevicompactum,P. chrysogenum,Scytalidium thermophilum andTrichoderma viride. Sera of 11 of 14 workers engaged in routine spawning of compost in tunnels reacted positively with 1 or more of the actinomycetes. Their10log serum titres increased with the duration of employment to an upper limit of 2.5. The sera of 19 non-exposed individuals were negative. Because high numbers of spores ofE. flexuosa,T. alba,T. curvata andT. fusca were present in the air that was used for successful inhalation provocation of mushroom workers with MWL and because of the elevated serum titres of these workers, we presume these organisms to contribute in the occurrence of MWL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103705
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  • 3
    Electronic Resource
    Electronic Resource
    Detection of potato virus Y in potato tubers: a comparison of polymerase chain reaction and enzyme-linked immunosorbent assay (1993)
    Springer
    Potato research 36 (1993), S. 13-20 
    Details
    ISSN: 1871-4528
    Keywords: reverse transcription ; PCR ; immunoassay ; virus detection ; ELISA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method was devised by which specific sequences of potato virus Y (PVY) RNA could be detected in total tuber RNA extracts by reverse transcription into cDNA and amplification by polymerase chain reaction (PCR). This method of PVY detection was tested and compared with the antibody-trapped antigen form of enzyme-linked immunosorbent assay (ATA-ELISA) using monoclonal antibodies. Both PCR and ATA-ELISA could detect PVY reliably in progeny tubers taken from growing plants of cv. Record, or tubers stored for 3 weeks after harvest. The ability to detect PVY decreased substantially after tubers had been stored for 20 weeks at 10°C. ATA-ELISA detected virus in only half the tubers from infected plants. However, PCR detected PVY very inefficiently in infected tubers after 20 weeks storage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02359829
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  • 4
    Electronic Resource
    Electronic Resource
    Acquisition of potato leafroll virus byMyzus persicae from secondarily-infected potato plants of different genotypes (1993)
    Springer
    Potato research 36 (1993), S. 89-96 
    Details
    ISSN: 1871-4528
    Keywords: ELISA ; enzyme amplification ; feeding behaviour ; field resistance ; honeydew excretion ; Myzus persicae ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The acquisition of potato leafroll virus (PLRV) byMyzus persicae nymphs from the top leaves of potato plants was studied throughout a growing season in relation to the antigen titre in those leaves and the feeding behaviour of the aphid. Secondarily-infected plants of eight potato genotypes with different levels of field resistance served as virus sources. Early in the growing season, plants were efficient sources for virus acquisition. The amount of viral antigen detected inM. persicae nymphs fed on the top leaves was strongly correlated with the titres of viral antigen in these leaves. Virus acquisition from the top leaves of older potato plants was markedly impaired and could not be correlated with their virus titre. With increasing age of the potato plants and the development of virus symptoms, the virus titre in the leaves declined and the initial weak correlation between the virus titre and field resistance ratings disappeared. Thus, screening secondarily-infected potato plants for field resistance to PLRV based on the concentration of viral antigen in leaves or in aphids fed on them should be avoided later in the growing season. The feeding rate ofM. persicae, measured by the number of honeydew droplets excreted, did not account for the reduced uptake of virus from older plants since it was not influenced by the age of the plant. Throughout the growing season, the feeding rate ofM. persicae nymphs on PLRV-infected plants was higher on genotypes with low levels of field resistance to PLRV than on genotypes with high ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358723
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  • 5
    Electronic Resource
    Electronic Resource
    Development of an ELISA for estimation of the copper nutritional status of wheat and cotton (1993)
    Springer
    Plant and soil 155-156 (1993), S. 453-456 
    Details
    ISSN: 1573-5036
    Keywords: copper deficiency ; cotton ; ELISA ; Gossypium hirsutum ; immunoassay ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies were carried out with hydroponically grown wheat and cotton to develop the Cu-requiring protein phenolase as a biomarker of Cu nutrient status. Isozymes of phenolase whose levels were reduced by Cu deficiency were identified by Western blots. A competitive enzyme-linked, immunosorbent assay (ELISA) was developed that could detect as little as 25 ng of phenolase. The ELISA revealed that Cu-sufficient cotton leaves had about 4-fold more phenolase antigen than did Cu-sufficient wheat leaves. In both species, the level of phenolase was reduced by 2- to 5-fold in leaves of Cu-deficient plants. Because the immunoassay for phenolase protein is rapid, inexpensive, and can be carried out with small amounts of leaf material, it has potential as a tool for assessment of the Cu status of crop plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025081
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  • 6
    Electronic Resource
    Electronic Resource
    An ELISA-disc procedure for antibodies toPseudomonas pseudomallei: application for a serological study of melioidosis in an endemic area (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 91-96 
    Details
    ISSN: 1573-0972
    Keywords: ELISA ; exotoxin ; immunodiagnosis ; melioidiosis ; Pseudomonas pseudomallei ; serum agglutination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The optimization and development of an ELISA-disc procedure for the detection of antibodies to whole cell surface antigens and purified exotoxin ofPseudomonas pseudomallei is described. Comparison of the serum agglutination test (SAT), the serum based enzyme-linked immunosorbent assay (ELISA) and the ELISA-disc procedures used on goat and human sera demonstrated a high correlation in their ability to detect antibodies specific forP. pseudomallei antigens. A serological survey using the ELISA-disc method was carried out on a normal human population in Sabah, Malaysia, an area known to be endemic for melioidosis. The prevalances of antibodies towards cell surface antigens and exotoxin ofP. pseudomallei were 28% and 8%, respectively. As a procedure, the ELISA-disc technique reported here is technically simple and provides savings in costs and is thus deemed suitable for seroepidemiological surveillance of melioidosis in remote areas of South-East Asia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00656525
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  • 7
    Electronic Resource
    Electronic Resource
    The wall associated lipoteichoic acid ofStreptococcus sanguis (1993)
    Springer
    Antonie van Leeuwenhoek 63 (1993), S. 29-34 
    Details
    ISSN: 1572-9699
    Keywords: lipoteichoic acid ; ELISA ; bacterial surface ; Streptococcus sanguis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A competitive ELISA is described for the measurement of lipoteichoic acid. The assay was used to determine the wall associated lipoteichoic acid ofStreptococcus sanguis which was found to represent only 2–4% of the phenol extractable content. Extracellular lipoteichoic acid was detected even after exhaustive cell washing. This material was not the result ofde novo synthesis because membrane de-polarization had no effect on the amount detected. Since extracellular lipoteichoic acid interfered with the measurement of cell surface antigen, cells were fixed with glutaraldehyde prior to assay. Lipoteichoic acid was demonstrated on the surface of fixed cells which did not leak antigen. The relevance of fixation used in antigen location studies by electron microscopy of immune-labelled cells is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00871728
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