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  • cDNA  (2)
  • flight  (2)
  • Springer  (4)
  • American Chemical Society
  • Oxford University Press
  • 1990-1994  (4)
  • 1980-1984
  • 1993  (4)
Collection
Publisher
  • Springer  (4)
  • American Chemical Society
  • Oxford University Press
Years
  • 1990-1994  (4)
  • 1980-1984
Year
  • 1
    ISSN: 1573-5028
    Keywords: barley ; cold ; cDNA ; EF-1α ; elongation factor 1α ; low temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone (pBLT63) encoding a protein synthesis elongation factor 1α (EF-1α) was isolated from a low-temperature winter barley shoot meristem library by differential screening. The nucleotide sequence of the coding region of the low-temperature-induced barley gene shows very high homology with two EF-1α plant genes from tomato and Arabidopsis. The barley genome contains an EF-1α gene family situated on the short arm of chromosome 2 and the long arm of chromosome 5. The nucleotide sequence data reported will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession number Z23130.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5028
    Keywords: cold ; low temperature ; barley ; gene expression ; cDNA ; shoot meristem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone of the previously unreported low-temperature-induced gene blt101 was isolated after a differential screen of a cDNA library prepared from low-temperature (6 °C day/2 °C night) grown barley shoot meristems. Southern blot analysis of barley ditelosomic addition lines was used to assign this single-copy gene to the long arm of chromosome 4. Analysis of steady-state levels of blt101 mRNA showed the induction of this transcript in shoot meristems upon transfer of barley (cv. Igri) plants from control (20 °C/15 °C) to low (6 °C/2 °C) temperature treatment. Further, the high level of this transcript is maintained at low temperatures but is reduced on transfer from low to control temperatures. The gene is not induced by drought or by foliar application of ABA. Analysis of segregating doubled haploid lines shows that there is no specific association of this gene with either spring/winter growth habit or frost hardiness. Examination of the spatial expression pattern revealed ubiquitous expression of blt101 in low-temperature (6 °C/2 °C) grown barley shoot meristems, mature leaves and roots.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-4927
    Keywords: butterfly ; flight ; low temperature ; Pgi locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Differences in genetic structure of samples of monarch butterflies caught at different times of day have been reported previously. This paper compares differences in allele and heterozygote frequencies at thePgi locus between animals flying early and those flying late in outdoor flight cages and between animals able and animals unable to fly at a constant low temperature. There were consistent effects across a number of tests and in comparisons with field data, especially in males. Animals with the Mallele were more likely to be able to fly at low temperatures, to become active early in outdoor flight cages, and to be caught early in the field. Also, differences were observed between males and females in the effect of allele on flight activity.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4927
    Keywords: butterfly ; flight ; low temperature ; Pgi locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Differences in genetic structure of samples of monarch butterflies caught at different times of day have been reported previously. This paper compares differences in allele and heterozygote frequencies at thePgi locus between animals flying early and those flying late in outdoor flight cages and between animals able and animals unable to fly at a constant low temperature. There were consistent effects across a number of tests and in comparisons with field data, especially in males. Animals with the Mallele were more likely to be able to fly at low temperatures, to become active early in outdoor flight cages, and to be caught early in the field. Also, differences were observed between males and females in the effect of allele on flight activity.
    Type of Medium: Electronic Resource
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