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  • Column liquid chromatography  (17)
  • ELISA  (7)
  • Springer  (24)
  • American Chemical Society
  • American Institute of Physics
  • American Institute of Physics (AIP)
  • Nature Publishing Group
  • Oxford University Press
  • Wiley
  • 2020-2022
  • 1990-1994  (24)
  • 1993  (24)
Collection
Keywords
Publisher
  • Springer  (24)
  • American Chemical Society
  • American Institute of Physics
  • American Institute of Physics (AIP)
  • Nature Publishing Group
    • +
Years
  • 2020-2022
  • 1990-1994  (24)
Year
  • 1
    Electronic Resource
    Electronic Resource
    A particle size distribution analysis of used HPLC column packing material (1993)
    Springer
    Chromatographia 35 (1993), S. 295-301 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Used column packings ; Particle size distribution ; Scanning electron microscopy ; Column age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Particle size distribution analysis and scanning electron microscopy (SEM) were carried out on eight used HPLC columns containing either irregular silica based, spherical silica based or spherical polymer based packing material. Particle size distributions of the used irregular silica based columns were at least bimodat at the outlet ends and either biomodal or log-normal at the inlet ends with regular progressions between the two extremes through the column. A new ODS-3 column showed log-normal size distributions from the inlet to the outlet ends. Spherical silica based column particle size distributions showed distinct shoulders on large central distribution peaks in most column sections with various degrees of shoulder erosion. The spherical resin based column showed a broader inlet particle size distribution progressing to a very narrow outlet distribution. SEMs of both irregular and spherical silica based columns revealed a larger number of undersized particles and debris at the outlet than inlet ends which could have resulted from stationary phase degradation, since this was not seen in the new ODS-3 column. While several SEMs of the spherical silica based columns revealed hollow spheres and twins, the spherical resin based column packing showed stress fractures or wrinkle lines resulting from use or dehydration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02277513
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  • 2
    Electronic Resource
    Electronic Resource
    Serological grouping of indigenous Bradyrhizobium sp. (Arachis) isolated from various soils of Thailand (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 635-640 
    Details
    ISSN: 1573-0972
    Keywords: Antigens ; antisera ; Arachis ; Bradyrhizobium ; ELISA ; peanut ; serological groups
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract ELISA and antibody adsorption tests were applied to determine the minimal somatic antigen constitution of 243 strains of Bradyrhizobium sp. (Arachis) using 12 antisera. The 243 indigenous bradyrhizobial isolates were from 15 sites in four regions of Thailand. A total of 29 serogroups were identified. Most (80%) of the isolates tested had at least one heat-stable antigen in common with strain 280A, forming a so-called 280A serocluster. At 11 of 15 sites tested, 53 to 100% of the isolates fell into one or two predominant serogroups. The serological properties of the indigenous bradyrhizobia were not related to the cropping history of the cultivated fields from which they were isolated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369570
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  • 3
    Electronic Resource
    Electronic Resource
    Mushroom worker's lung: Serologic reactions to thermophilic actinomycetes present in the air of compost tunnels (1993)
    Springer
    Mycopathologia 122 (1993), S. 21-28 
    Details
    ISSN: 1573-0832
    Keywords: Actinomycetes ; Allergy ; Compost ; ELISA ; Lung disease ; Mushroom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Vast numbers of spores of the thermophilic actinomycetesExcellospora flexuosa,Thermomonospora alba,T. curvata andT. fusca were collected from the air in fermentation tunnels during the spawning of mushroom compost, i.e. over 109 CFU m−3 of air. Five different genera of fungi, namely,Aspergillus,Aureobasidium,Cladosporium,Penicillium andScytalidium, were found at only 103 CFU m−3 of air.Agaricus bisporus, used for spawning, was absent. Sera of 10 mushroom growers affected by Mushroom Worker's Lung (MWL) were tested by a qualitative dot-ELISA for antibodies against the spores of these micro-organisms. All 10 were positive for one or more of the actinomycetesE. flexuosa,T. alba,T. curvata andT. fusca. No antibodies were found againstStreptomyces thermovulgaris,Thermoactinomyces vulgaris andT. sacchari nor against the fungiAspergillus fumigatus,Penicillium brevicompactum,P. chrysogenum,Scytalidium thermophilum andTrichoderma viride. Sera of 11 of 14 workers engaged in routine spawning of compost in tunnels reacted positively with 1 or more of the actinomycetes. Their10log serum titres increased with the duration of employment to an upper limit of 2.5. The sera of 19 non-exposed individuals were negative. Because high numbers of spores ofE. flexuosa,T. alba,T. curvata andT. fusca were present in the air that was used for successful inhalation provocation of mushroom workers with MWL and because of the elevated serum titres of these workers, we presume these organisms to contribute in the occurrence of MWL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103705
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of potato virus Y in potato tubers: a comparison of polymerase chain reaction and enzyme-linked immunosorbent assay (1993)
    Springer
    Potato research 36 (1993), S. 13-20 
    Details
    ISSN: 1871-4528
    Keywords: reverse transcription ; PCR ; immunoassay ; virus detection ; ELISA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method was devised by which specific sequences of potato virus Y (PVY) RNA could be detected in total tuber RNA extracts by reverse transcription into cDNA and amplification by polymerase chain reaction (PCR). This method of PVY detection was tested and compared with the antibody-trapped antigen form of enzyme-linked immunosorbent assay (ATA-ELISA) using monoclonal antibodies. Both PCR and ATA-ELISA could detect PVY reliably in progeny tubers taken from growing plants of cv. Record, or tubers stored for 3 weeks after harvest. The ability to detect PVY decreased substantially after tubers had been stored for 20 weeks at 10°C. ATA-ELISA detected virus in only half the tubers from infected plants. However, PCR detected PVY very inefficiently in infected tubers after 20 weeks storage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02359829
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  • 5
    Electronic Resource
    Electronic Resource
    Acquisition of potato leafroll virus byMyzus persicae from secondarily-infected potato plants of different genotypes (1993)
    Springer
    Potato research 36 (1993), S. 89-96 
    Details
    ISSN: 1871-4528
    Keywords: ELISA ; enzyme amplification ; feeding behaviour ; field resistance ; honeydew excretion ; Myzus persicae ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The acquisition of potato leafroll virus (PLRV) byMyzus persicae nymphs from the top leaves of potato plants was studied throughout a growing season in relation to the antigen titre in those leaves and the feeding behaviour of the aphid. Secondarily-infected plants of eight potato genotypes with different levels of field resistance served as virus sources. Early in the growing season, plants were efficient sources for virus acquisition. The amount of viral antigen detected inM. persicae nymphs fed on the top leaves was strongly correlated with the titres of viral antigen in these leaves. Virus acquisition from the top leaves of older potato plants was markedly impaired and could not be correlated with their virus titre. With increasing age of the potato plants and the development of virus symptoms, the virus titre in the leaves declined and the initial weak correlation between the virus titre and field resistance ratings disappeared. Thus, screening secondarily-infected potato plants for field resistance to PLRV based on the concentration of viral antigen in leaves or in aphids fed on them should be avoided later in the growing season. The feeding rate ofM. persicae, measured by the number of honeydew droplets excreted, did not account for the reduced uptake of virus from older plants since it was not influenced by the age of the plant. Throughout the growing season, the feeding rate ofM. persicae nymphs on PLRV-infected plants was higher on genotypes with low levels of field resistance to PLRV than on genotypes with high ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358723
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  • 6
    Electronic Resource
    Electronic Resource
    High-performance liquid chromatographic separation of fullerene mixtures using a novel stationary phase (1993)
    Springer
    Chromatographia 35 (1993), S. 631-636 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Fullerenes ; FullereneSep® ; Separation factor ; HPLC-particle beam mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary High-performance liquid chromatographic separation of fullerene mixtures in the range C60 to C100 can be achieved using a novel stationary phase which rivals the performance of the more expensive ‘Pirkle-type’ columns currently employed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02267928
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  • 7
    Electronic Resource
    Electronic Resource
    HPLC determination of pyroglutamic acid as a degradation product in parenteral amino acid formulations (1993)
    Springer
    Chromatographia 36 (1993), S. 75-78 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Glutamine ; Pyroglutamic acid ; Amino acids formulations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The aminoacid glutamine in aqueous solution and in conditions of high temperature and long term storage is partly transformed into pyroglutamic acid which exhibits potential neurotoxic effects. Commercially available aminoacid mixtures supplemented with glutamine are heat-sterilized and some losses of glutamine and formation of pyroglutamic acid may occur. The aim of the work was to set up an easy and reliable HPLC method which allows the determination of pyroglutamic acid as a degradation product of glutamine. The column was a 5 μm Hypersil ODS (100×4.6 mm) and the mobile phase 100% 0.007 M phosphate buffer pH 3.5. Stability studies in different conditions of temperature and time of storage were performed on aminoacid mixture available in the commerce.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02263841
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  • 8
    Electronic Resource
    Electronic Resource
    A micro-scale method employing surface plasmon resonance detection for the determination of conditions for immunoaffinity chromatography of proteins (1993)
    Springer
    Chromatographia 35 (1993), S. 45-49 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Immunoaffinity chromatography ; Proteins ; Surface plasmon resonance detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The BIAcoreTM Biosensor System utilizes a detection principle known as Surface Plasmon Resonance (SPR) which, in simple terms, detects changes in the refractive index of a solution in contact with a gold film. The gold film is surface modified with carboxymethylated dextran to produce a hydrophillic matrix onto which macromolecules may be covalently immobilized. In this respect, the BIAcoreTM is similar to any other insoluble matrix, such as a chromatographic support. The SPR detection principle, however, allows one to directly ‘visualize’ the interaction under study, in real time and without the need for reporter molecules such as enzyme-labels. In addition, the very small sample requirements, automated robotics unit and ease of data analysis suggest the potential use of the BIAcoreTM instrumentation for assay development and possibly process development, especially where bio-specific interactions such as immunoaffinity chromatography are used as a step in the process. In this report, we demonstrate the use of the BIAcoreTM SPR detector for the micro-scale determination of conditions for immunoaffinity chromatography of soluble complementreceptor 1, sCR1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02278555
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  • 9
    Electronic Resource
    Electronic Resource
    Ion-pair LC of carbohydrates at alkaline pH. Separation and retention behaviour of glycoconjugates (1993)
    Springer
    Chromatographia 35 (1993), S. 55-60 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbohydrates ; Ion-pair separation ; Glycoconjugates ; PRP-1 and PLRP-S
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A new technique for the separation of carbohydrates as ion-pairs in strongly alkaline solution is presented. Carbohydrates are weakly acidic and partly present as anions at pH≥12 [1]. They are retained as ion-pairs on polymeric solid phases (PRP-1 and PLRP-S) with a hydrophobic quaternary ammonium counter ion present in the mobile phase. The effects of nature and concentration of mobile phase components on the retention of carbohydrates have been investigated and an ion-pair distribution model is proposed. The influence of temperature indicated no changes in retention mechanism with high counter ion concentration, but the resolution decreased with increasing temperature. Saccharides added to the mobile phase were shown to increase the retention and the selectivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02278557
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  • 10
    Electronic Resource
    Electronic Resource
    HPLC determination of 18 β-glycyrrhetinic and glycyrrhizinic acids in toothpastes after solid phase extraction (1993)
    Springer
    Chromatographia 35 (1993), S. 167-172 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; 18 β-glycyrrhetinic acid ; Glycyrrhizinic acid ; Toothpastes ; Solid phase extraction (SPE)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary HPLC methods suitable for the selective and sensitive determination of 18 β-Glycyrrhetinic acid (GT) and Glycyrrhizinic acid (GZ) in toothpastes have been developed. The methods involve a preliminary quantitative solid phase extraction (C-18 sorbent) for sample clean-up and analyte concentration. Chromatographic separations were performed on reversed phase (C-8; C-18) columns using mixtures of methanol and phosphate buffers (pH 3.0) as the mobile phase under isocratic or gradient elution conditions. The method were applied successfully to the analysis of commercial toothpastes containing low levels (0.013–0.065%) of GT and GZ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269697
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