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  • Animals  (2)
  • Male  (1)
  • GENERAL
  • Organic Chemistry
  • ddc:330
  • 1990-1994  (2)
  • 1992  (2)
  • 1
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    Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1992-02-28
    Description: Novel sol-gel synthetic techniques were used to immobilize copper-zinc superoxide dismutase (CuZnSOD), cytochrome c, and myoglobin (Mb) by encapsulation in stable, optically transparent, porous silica glass matrices under mild conditions such that the biomolecules retained their characteristic reactivities and spectroscopic properties. The resulting glasses allowed transport of small molecules into and out of the glasses at reasonable rates but nevertheless retained the protein molecules within their pores. Chemical reactions of the immobilized proteins could be monitored by means of changes in their visible absorption spectra. Silica glasses containing the immobilized proteins were observed to have similar reactivities and spectroscopic properties to those found for the proteins in solution. For example, encapsulated CuZnSOD was demetallated and remetallated, encapsulated ferricytochrome c was reduced and then reoxidized, and encapsulated met Mb was reduced to deoxy Mb and then reacted either with dioxygen to make oxy Mb or with carbon monoxide to make carbonyl Mb.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ellerby, L M -- Nishida, C R -- Nishida, F -- Yamanaka, S A -- Dunn, B -- Valentine, J S -- Zink, J I -- GM28222/GM/NIGMS NIH HHS/ -- T32 GM08375/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1992 Feb 28;255(5048):1113-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Biochemistry, University of California, Los Angeles 90024.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1312257" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cattle ; Cytochrome c Group/chemistry ; Gels ; *Glass ; Horses ; Myoglobin/chemistry ; Proteins/*chemistry ; Solutions ; Spectrum Analysis ; Superoxide Dismutase/chemistry
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Expression directed from HIV long terminal repeats in the central nervous system of transgenic mice (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1992-12-11
    Description: Infection with the human immunodeficiency virus (HIV) is frequently accompanied by the AIDS (acquired immunodeficiency syndrome) dementia complex. The role of specific HIV genetic elements in the pathogenesis of central nervous system (CNS) disease is not clear. Transgenic mice were constructed that contained the long terminal repeats (LTRs) of two CNS-derived strains and a T cell tropic strain of HIV-1. Only mice generated with CNS-derived LTRs directed expression in the CNS, particularly in neurons. Thus, some strains of HIV-1 have a selective advantage for gene expression in the brain, and neurons can supply the cellular factors necessary for their transcription.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Corboy, J R -- Buzy, J M -- Zink, M C -- Clements, J E -- AI27297/AI/NIAID NIH HHS/ -- AI28748/AI/NIAID NIH HHS/ -- NS07000/NS/NINDS NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1992 Dec 11;258(5089):1804-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Comparative Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1465618" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Brain/*physiology ; Central Nervous System/*physiology ; Female ; Gene Expression ; *HIV Long Terminal Repeat ; HIV-1/*genetics ; Intestine, Small/physiology ; Lung/physiology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Mice, Transgenic ; Ocular Physiological Phenomena ; Organ Specificity ; RNA, Messenger/analysis/*metabolism ; Recombinant Fusion Proteins/metabolism ; Spinal Cord/physiology ; Thymus Gland/physiology ; beta-Galactosidase/genetics/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
    Location Call Number Expected Availability
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