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  • BHK cells  (1)
  • Biosynthesis  (1)
  • COMPUTER PROGRAMMING AND SOFTWARE  (1)
  • Humans
  • Life Sciences (General)
  • Life and Medical Sciences
  • 2000-2004
  • 1990-1994  (5)
  • 1935-1939
  • 1992  (5)
Collection
Keywords
Publisher
Years
  • 2000-2004
  • 1990-1994  (5)
  • 1935-1939
Year
  • 1
    Electronic Resource
    Electronic Resource
    Determination of the rate-limiting step(s) in the biosynthetic pathways leading to penicillin and cephalosporin (1992)
    Springer
    Journal of industrial microbiology and biotechnology 10 (1992), S. 157-163 
    Details
    ISSN: 1476-5535
    Keywords: Penicillin ; Cephalosporin ; Biosynthesis ; Rate-limiting step(s)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary This paper is a review of strategies that have been used, or that could be used, to determine the rate-limiting step(s) in the biosynthetic pathways leading to penicillin or cephalosporin. Information is summarized from published material that involves studies with low-producing strains ofPenicillium chrysogenum andCephalosporium acremonium. We also summarize information derived from some high-producing production strains. Identification of the rate-limiting step(s) was of great interest to us as the first step in a rational program to further improve antibiotic titers of these highly developed strains. A number of approaches that could be used to elucidate the rate-limiting step(s) are described herein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569760
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Expression, glycosylation and secretion of yeast acid phosphatase in hamster BHK cells (1992)
    Springer
    Glycoconjugate journal 9 (1992), S. 39-44 
    Details
    ISSN: 1573-4986
    Keywords: yeast acid phosphatase ; glycosylation ; transfection ; BHK cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The gene PHO5 coding for one of the repressible acid phosphatases of the yeastSaccharomyces cerevisiae has been expressed at high efficiency in the baby hamster kidney (BHK) cell line. The expression vector was constructed from PHO5 driven by the human β-actin promoter and was transfected into BHK cells by the calcium phosphate method. The recombinant APase (r-APase) which was secreted in active form from the cells was estimated by SDS/polyacrylamide gel electrophoresis to have molecular massM r=62000, indicating substitution of the polypeptide moiety by 2–3 asparagine-linked glycans. Analysis by sequential lectin affinity chromatography of glycopeptides obtained from r-APase with Pronase showed that the glycans are predominantly of the 2.2.4 triantennary and tetraantennary complex-type. These data suggest that the extensive glycosylation of yeast APase, which contains eight polymannose substituents, is not essential for secretion and expression of enzymatic activity of the transfected gene product.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00731176
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    E.coli hemolysin interactions with prokaryotic and eukaryotic cell membranes (1992)
    New York, NY : Wiley-Blackwell
    BioEssays 14 (1992), S. 519-525 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The hemolysin toxin (HlyA) is secreted across both the cytoplasmic and outer membranes of pathogenic Escherichia coli and forms membrane pores in cells of the host immune system, causing cell dysfunction and death. The processes underlying the interaction of HlyA with the bacterial and mammalian cell membranes are remarkable. Secretion of HlyA occurs without a periplasmic intermediate and is directed by an uncleaved C-terminal targetting signal and the HlyB and HlyD translocator proteins, the former being a member of a transporter superfamily central to import and export of a wide range of substrates by prokaryotic and eukaryotic cells. The separate process by which HlyA is targetted to mammalian cell membranes is dependent upon fatty acylation of a non-toxic precursor, proHlyA. This is achieved by a novel mechanism directed by the activator protein HlyC, which binds to an internal proHlyA recognition sequence and provides specificity for the transfer of fatty acid from cellular acyl carrier protein.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950140804
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    Paper (German National Licenses)
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  • 4
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    Effects of dimethyl prostaglandin A1 on herpes simplex virus and human immunodeficiency virus replication (1992)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: We have investigated the direct effect of dimethyl prostaglandin A1 (dmPGA1) on the replication of herpes simplex virus (HSV) and human immunodeficiency virus type 1 (HIV-1). dmPGA1 significantly inhibited viral replication in both HSV and HIV infection systems at concentrations of dmPGA1 that did not adversely alter cellular DNA synthesis. The 50% inhibitory concentration (ID50) for several HSV type 1 (HSV-1) strains ranged from 3.8 to 5.6 micrograms/ml for Vero cells and from 4.6 to 7.3 micrograms/ml for human foreskin fibroblasts. The ID50s for two HSV-2 strains varied from 3.8 to 4.5 micrograms/ml for Vero cells; the ID50 was 5.7 micrograms/ml for human foreskin fibroblasts. We found that closely related prostaglandins did not have the same effect on the replication of HSV; dmPGE2 and dmPGA2 caused up to a 60% increase in HSV replication compared with that in untreated virus-infected cells. HIV-1 replication in acutely infected T cells (VB line) and chronically infected macrophages was assessed by quantitative decreases in p24 concentration. The effective ID50s were 2.5 micrograms/ml for VB cells acutely infected with HIV-1 and 5.2 micrograms/m for chronically infected macrophages. dmPGA1 has an unusual broad-spectrum antiviral activity against both HSV and HIV-1 in vitro and offers a new class of potential therapeutic agents for in vivo use.
    Keywords: Life Sciences (General)
    Type: Antimicrobial agents and chemotherapy (ISSN 0066-4804); Volume 36; 10; 2253-8
    Format: text
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    NASA TECHNICAL REPORTS
  • 5
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    Analysis of a virtual memory model for maintaining database views (1992)
    In:  Other Sources
    Details
    Publication Date: 2019-07-12
    Description: This paper presents an analytical model for predicting the performance of a new support strategy for database views. This strategy, called the virtual method, is compared with traditional methods for supporting views. The analytical model's predictions of improved performance by the virtual method are then validated by comparing these results with those achieved in an experimental implementation.
    Keywords: COMPUTER PROGRAMMING AND SOFTWARE
    Type: IEEE Transactions on Software Engineering (ISSN 0098-5589); 18; 5 Ma; 402-409
    Format: text
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    NASA TECHNICAL REPORTS
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