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  • Oxford University Press  (35)
  • Blackwell Publishing Ltd  (28)
  • 2005-2009  (27)
  • 1990-1994  (36)
  • 2006  (27)
  • 1992  (36)
  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The unit-copy P1 plasmid depends for stability on a plasmld-encoded partition region called par, consisting of the parA and parB genes and the parS site. ParA is absolutely required for partition, but its partition-critical role is not known. Purified ParA protein is shown to possess an ATPase activity in vitro which is specifically stimulated by purified ParB protein and by DNA. ParA is responsible for regulation of expression of parA and parB, and purified ParA has an ATP-dependent, site-specific DNA binding activity which recognizes a sequence that overlaps the parA promoter. The rote of the ATP-dependence of the binding activity, as well as other possible functions of the ATPase activity in partition, is discussed.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The amdR (intA) regulatory gene of Aspergillus nidulans encodes a 765-amino-acid polypeptide which determines the omega-amino acid induction of at least five structural genes. The AmdR polypeptide contains a potential Zn(ll)2Cys6 DNA-binding motif which has been shown to be present in the N-terminal region of a large number of fungal activator proteins. In vitro mutagenesis of the fourth cysteine of this motif abolishes AmdR function as shown by loss of complementation of an amdR− mutation and by the AmdR− phenotype of a mutant gene replacement strain. Studies using constructs in which the proposed AmdR DNA-binding motif is replaced with that from another activator, FacB, shows that induction is independent of DNA-binding specificity and that sequences in the C-terminal region of AmdR are activation domains. Sequencing of several amdR mutant alleles which affect activation and/or induction, together with studies of deletion constructs indicate that changes in the conformation of the protein determines its activity and that this is modulated by inducers.
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  • 13
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The broad-host-range plasmid RK2 has been shown to encode several proteins important for its maintenance within bacterial populations of a number of Gram-negative bacteria. Their genes are organized into two operons: parCBA and parD. These operons have been proposed to be transcribed from two divergent promoters, p-parCBA and p-parD, located within a sequence of approximately 150 bases. In this report we identify and characterize the sequences required for regulated transcription from these promoters in Escherichia coli, Agrobacterium tumefaciens and Pseudomonas aeruginosa. Both of these promoters are repressed by their own gene products in the same manner in all three bacteria tested, with ParA functioning as the primary repressor of p-parCBA and ParD functioning as the repressor of p-parD. The binding regions of these proteins were determined through deletion analyses, DNA mobility shift assays, and an examination of the effect of mutations in this region. Based on these observations, the ParA protein appears to bind to either two inverted repeat or two direct repeat sequences, one downstream from the transcriptional initiation site and the other upstream of the p-parCBA -35 box. The ParD protein appears to bind to one inverted repeat sequence, located between the -35 and -10 boxes of p-parD.
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  • 14
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 23 (1992), S. 0 
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Reproductive performance of albino and pigmented channel catfish (Ictalurus punctarus) females was compared when spawning was induced with HCG (human chorionic gonadotropin) or Ovaprim/〉 (domperidone and [d-Ala6, Pro9 NEt]-luteinizing hormone-releasing hormone). In 1985, only HCG was used to induce spawning. Albino females required more injections, had a longer latency period from time of first injection to spawning, and had lower spawning success (30%) than pigmented females (77%). No differences were observed between albino and pigmented females for time-of-day of spawning, number of eggs obtained by manual stripping, and number of eggs/kg body weight. In 1988, HCG, Ovaprim, and saline (control) were used to induce spawning. HCG induced spawning in 80% of albino and 71% of pigmented females. Ovaprim induced 67% of pigmented females to spawn, but only 2 of 5 albino females spawned. One of four pigmented females injected with saline spawned. Albino females yielded 40–50% fewer eggs/kg body weight than pigmented females. Other performance characteristics for albino and pigmented females were similar whether injected with HCG or Ovaprim. High temperatures encountered during the 1985 spawning season my have had a differential effect on albino and pigmented fish and may account for some differences in spawning success.
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  • 15
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 23 (1992), S. 0 
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two experiments were conducted to evaluate the dietary iron requirement of juvenile Penaeus vunnamei. Prior to initiation of 28 day (Experiment I) and 35 day (Experiment II) feeding trials, 17–20day-old postlarvae were fed the basal diet lacking iron supplementation but containing 12 mg Fe/kg for seven days. After conditioning, juvenile shrimp (mean weight: 0.038 g Experiment I, 0.047 g Experiment II) were fed one of four diets supplemented with 0, 20, 40 or 80 mg Fe/kg from ferrous sulfate heptahydrate. Final weight gain of shrimp ranged from 1.03 to 1.28 g in Experiment I and 1.62 to 1.94 g in Experiment II. Although percent weight gain was in excess of 2700% in Experiment I and 3400% in Experiment II, there were no significant differences in growth or survival of shrimp fed the different levels of iron. Iron levels of the hepatopancreas and carapace increased linearly with dietary iron; however, there were no significant differences in the iron content of muscle tissue. Based on these results, the iron content of the basal diet appeared to satisfy physiological needs of the shrimp. Therefore, practical diets containing protein sources of animal origin which are rich in iron should not require iron supplementation.
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  • 16
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 40 (1992), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The killifish Rivulus marmoratus is the only known selfing hermaphroditic vertebrate, and males of the species are usually exceedingly rare or non-existent in nature. Collections on several Belize cays in 1988 and 1989 yielded 13.5–24% males. Factors responsible for this unusually high proportion of males are currently not understood. Likewise, the biological significance of males in populations which otherwise consist of selfing hermaphrodites (with internal fertilization) is problematic and awaits further study.
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  • 17
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Family business review 5 (1992), S. 0 
    ISSN: 1741-6248
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Sociology , Economics
    Notes: Providing clear goals for a company and communicating them are among the most powerful means for guiding the behavior of the people in an organization. In this article, we explore the range of objectives or goals of family-owned and -managed companies and identify those most commonly regarded as important by owner-managers. Further, we describe six major empirical dimensions of goals that we derived by factor-analytic procedures. Finally, we suggest how researchers, managers, and consultants can use our work to help owner-managers clarify and communicate their goals.
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  • 18
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 23 (1992), S. 0 
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Diets containing a complete mineral premix or mineral premixes from which Ca, P, Na, CI, K, Mg, Mn, Fe, Zn, Cu, I, Se, or Cr were individually deleted were fed to juvenile Penaeus vunnamei (mean initial weight 79.5 mg) over a 4-week experimental period. Final mean percent weight gain ranged from 1,472 (Cu-free mineral premix) to 1,991% (Na-free mineral premix) with an average of 1,812% for all treatments. Survival during the first two week period and the second two week period was greater than 92% and 88%, respectively. There were no significant differences observed among treatments for growth and survival data.Upon termination of the growth trial, the hepatopancreas and carapace were dissected from the shrimp and evaluated for ash, Ca, P, Na, K, Mg, Mn, Fe, Zn and Cu content. Of the minerals analyzed, the individual deletion of Mg, Mn, Fe, Zn and Cu from the mineral supplement resulted in reduced tissue mineralization in P. vannamei whereas, the deletion of Ca and P from the mineral mix did not produce significant responses. Deletion of Se from the mineral premix resulted in a significant increase in the ash content of the carapace. Based on the information provided by this and other studies, further investigation of the dietary requirements of P. vannamei for Ca, P, Fe, Mg, Mn, Zn, Cu and Se is recommended.
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  • 19
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 110 (1992), S. 0 
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: In a hydrothermally active ocean basin, vigorous hydrothermal circulation in highly permeable basement rocks maintains a nearly constant temperature at the base of the overlying accumulating sediment section. To investigate the thermal effects of sedimentation in such geological settings, we have developed a simple one-dimensional finite element model and applied it to cases in the northeast Pacific. The model accounts for differential motion of fluids and sediment grains during compaction, and can be used with any porosity-depth function. Results demonstrate clearly that the constant basal temperature of an accumulating sediment section, maintained by convective heat transfer in the basement, causes the section to remain thermally near steady state for even very high rates of accumulation, particularly when compared to conditions estimated for a section where heat is transported in the basement by conduction. A 10-kyr period of thermal recovery due to the highly diminished sediment supply during the post-Pleistocene further reduces the thermal effects of sedimentation by a significant amount. Only in rare cases where rates of accumulation exceed 10 mm yr−1 and sediment thicknesses exceed 1 km are the sea-floor heat flow and temperatures at depth diminished significantly. An example is found in Middle Valley of the Juan de Fuca Ridge, in a part of which over 2 km of sediment has accumulated in the past 200 kyr. Even in this extreme case, the heat flow is estimated to be lower than that of the steady state by about only 15 per cent. While rates of accumulation are also high in other parts of Middle Valley and in many other hydrothermally active areas, such as Guayamas Basin, Escanaba Trough and the eastern flank of the Juan de Fuca Ridge, these rates and the accumulated sediment thicknesses are found to be insufficient to cause appreciable thermal anomalies.
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  • 20
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Centaurus 35 (1992), S. 0 
    ISSN: 1600-0498
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Natural Sciences in General
    Notes: I have carried out a selective reworking of Chapter 32 of Kepler's Astronomia Nova - which was the source of all his physical ideas - and have established that no foundation exists either there, or subsequently, for what is nowadays known as ‘Kepler's distance law‘. By contrast, I show that his innovative approach led him to consider the component of velocity which is precisely perpendicular to the Sun-planet line, and so to formulate motion taking place instantaneously in a circle round the Sun; this is exactly equivalent to the area law according to modern standards. Moreover, no other interpretation would have been compatible with Kepler's Aristotelian principles.
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