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  • 1
    Electronic Resource
    Electronic Resource
    Impact on ammonia volatilization losses of mixing KCl of high pH with urea (1994)
    Springer
    Nutrient cycling in agroecosystems 40 (1994), S. 89-92 
    Details
    ISSN: 1573-0867
    Keywords: KCl ; ammonia volatilization ; urea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ammonia volatilization associated with urea hydrolysis has been shown to be primarily associated with the pH of the soil solution and its buffering ability in the immediate zone of the fertilizer granule. Numerous studies have also shown that these losses can be reduced significantly by the addition of large amounts of KCl with the urea. Because the pH of commercial sources of potash ranges from 6.5 to 9.5, investigations were conducted to determine if the high pH of these K sources had an effect on the ammonia lost from three contrasting soils. Despite large ammonia losses (approximately 50% of N applied) and a significant reduction in loss due to the use of KCl (30%-50% reduction), the experiments showed no effect of potash pH on ammonia loss. It may be concluded that no risk of enhanced ammonia loss can be associated with the use of high-pH potash sources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00750092
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  • 2
    Electronic Resource
    Electronic Resource
    The covariance of latitudinal and longitudinal motions of small magnetic features (1994)
    Springer
    Solar physics 151 (1994), S. 15-28 
    Details
    ISSN: 1573-093X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We study the covariance of longitudinal and latitudinal motions of small magnetic features after subtracting long-term averages of differential rotation and meridional flow. The covariance is generally interpreted as Reynolds stress and linked to the equatorward transport of angular momentum. Using high-resolution magnetograms taken daily with the NSO Vacuum Telescope on Kitt Peak, we determine large-scale motions by a two-dimensional crosscorrelation analysis of pairs of consecutive daily observations from which active regions are excluded, i.e., we analyze the motions of small magnetic features. In the present work, we focus on 107 day pairs obtained during the year 1988 and on 472 day pairs taken in selected intervals from 1978 to 1990. We find that all covariance values are very small (below 250 m2 s−2), which is about one to two orders of magnitude smaller than the values from sunspot measurements derived by other authors. At active region latitudes, the masking process increases the noise, which increases the chance that the covariances at these latitudes are not significantly different from zero. We find that the results depend strongly on the temporal averaging involved. Daily unaveraged crosscorrelations lead to no apparent correlation between the residual velocities, while in the monthly averages of the 1988 data, we find a covariance of −37 ± 15 m2 s−2 at 45° with a linear correlation of −0.59, which is significantly different from zero and has the right sign for an equatorial transport of angular momentum. When we average over longer time periods, the covariance values decrease again. The annual averages of the 1978–1990 data show both no significant covariances and the smallest errors. These small covariances imply that the motions of small magnetic features do not reflect the transport of angular momentum via the mechanism of Reynolds stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00654078
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  • 3
    Electronic Resource
    Electronic Resource
    Reproductive success in the Caribbean octocoral Briareum asbestinum (1992)
    Springer
    Marine biology 114 (1992), S. 157-163 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For most sessile marine invertebrates the number of offspring produced by an individual is often determined indirectly from counts of eggs present, with the assumption that all or most become viable offspring. Few field data on the actual number of offspring produced per individual (reproductive success) have been reported. We examined reproductive success in the octocoral, Briareum asbestinum (Pallas), by measuring embryo densities on 40 female colonies on two reefs (Pinnacles and House reefs) in the San Blas Islands, Panama from 1986 to 1988. The percentage of female colonies with mature eggs which subsequently released embryos was low, ranging from 46.6% in 1986 to 5.0% in 1988. Reproductive success was significantly different between reefs. Pinnacles reef had significantly more successful colonies (those releasing embryos) than House reef in all three years and significantly higher embryo densities in 1986 and 1987. There was a significant positive correlation between female reproductive success and the density and proximity of nearby males in both 1986 and 1987. In addition, fertile female branches placed 25 cm away from male branches produced significantly more embryos than female branches placed 50 cm away. In 1988 two groups of female branches were placed at distances of 〈50 cm and 5 m from four large male colonies at Pinnacles reef. Of the 20 female branches placed 5 m from the males none released embryos while 35% (7 of 20 branches) of the branches 〈50cm from males released embryos. These data indicate that simple counts of mature eggs present within female colonics prior to the reproductive season provide a poor estimate of reproductive success in B. ashestinum and that female reproductive success is positively influenced by the close proximity of males.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00350865
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  • 4
    Electronic Resource
    Electronic Resource
    DNA fingerprints of a gorgonian coral: a method for detecting clonal structure in a vegetative species (1992)
    Springer
    Marine biology 114 (1992), S. 317-325 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Clonal reproduction, a common life history strategy among sessile marine invertebrates, can lead to high local abundances of one to a few genotypes in a population. Analysis of the clonal structure of such populations can provide insight into the ecological and evolutionary history of the population, but requires markers that can identify individual genets. Forensic and demographic studies have demonstrated that DNA fingerprinting can provide markers that are unique for an individual genotype. We have generated DNA fingerprints for over 70 colonies of the clonal gorgonian, Plexaura A (Plexaura sp. A) collected from June 1990 through July 1991 in the San Blas Islands, Panama. DNA fingerprints within a singic individual were identical and fingerprinting resolved multiple genotypes within and among reefs. On one reef in the San Blas Islands, Panama, 59% of the colonies sampled were of one genotype and this genotype was not found on any other sampled reefs. A previous study using tissue grafts identified 13 putative clones on these reefs, while DNA fingerprints of the same colonies differentiated 17 genotypes. The present study demonstrates the utility of DNA fingerprinting for distinguishing clones and for identifying clonal structure of marine invertebrate populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349534
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  • 5
    Electronic Resource
    Electronic Resource
    Mandibular gland secretions of the male beewolvesPhilanthus crabroniformis, P. barbatus, andP. pulcher (Hymenoptera: Sphecidae) (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 27-37 
    Details
    ISSN: 1573-1561
    Keywords: Philanthus crabroniformis ; Philanthus barbatus ; Philanthus pulcher ; Hymenoptera ; Sphecidae ; beewolf ; mandibular glands ; pheromones ; semiochemicals ; mass spectrometry ; infrared spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The composition of the territorial marking pheromones from mandibular glands of males of the beewolvesPhilanthus crabroniformis, P. barbatus, andP. pulcher have been determined. The structures of the components were elucidated by gas chromatography-mass spectrometry and gas chromatography-Fourier transform infrared spectroscopy. The major compound ofP. crabroniformis is isopropyl tetradecanoate, with somewhat lesser amounts of 2-tridecanone, 3-methyl-3-butenyl tetradecanoate, and 92∶8 (Z)∶(E)-11-eicosen-1-ol. The major compounds ofP. barbatus are ethyl tetradecanoate and hexadecanal, which are present in approximately a 60∶40 ratio. These two compounds comprise over 95% of the neutral lipids. Also present in lesser amounts are ethyl dodecanoate, tetradecanal, hexadecan-1-ol, a Δ x -octadecen-1-ol, and octadecan-1-ol. The major compounds ofP. pulcher are ethyl (Z)-7-hexadecenoate and geranylgeraniol acetate, which comprise nearly 90% of the neutral lipid fraction, with smaller amounts of tetradecanal, pentadecanal, and ethyl hexadecanoate; trace amounts of Δ x hexadecenal, hexadecanal, and octadecanal are also present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00997162
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  • 6
    Electronic Resource
    Electronic Resource
    Wound-induced and developmental activation of a poplar tree chitinase gene promoter in transgenic tobacco (1994)
    Springer
    Plant molecular biology 25 (1994), S. 799-815 
    Details
    ISSN: 1573-5028
    Keywords: Populus ; cottonwood ; pollen ; GUS ; defense gene ; systemic response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wounding hybrid poplar (Populus trichocarpa × P. deltoides) trees results in the expression of novel wound-inducible (win) mRNAs thought to encode proteins involved in defense against pests and pathogens. Members of thewin6 gene family encode acidic multi-domain chitinases, with combined structure and charge characteristics that differ from previously described chitinases.Win6 expression has been shown to occur in pooled unwounded leaves of a wounded (on multiple leaves) poplar plant. Here we demonstrate that wounding a single leaf induceswin6 expression locally, in the wounded leaf, and remotely, in specific unwounded leaves with strong vascular connections to the wounded leaf. We also demonstrate that awin6 promoter-β-glucuronidase (GUS) gene fusion (win6-GUS) responds to wounding locally and remotely in transgenic tobacco. These data indicate that the poplarwin6 promoter has regulatory elements that are responsive to ‘wound signals’ in the heterologous host. In addition,win6-GUS is developmentally activated in unwounded young leaves and floral tissues of transgenic tobacco. Similar developmental expression patterns are found to occur forwin6 in poplar trees, demonstrating that a herbaceous plant can serve as a host for woody tree transgene analysis and can accurately predict expression patterns in tree tissues (e.g. flowers) that would be difficult to study in free-living trees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028875
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  • 7
    Electronic Resource
    Electronic Resource
    Adhesion of fungal spores and germlings to host plant surfaces (1994)
    Springer
    Protoplasma 181 (1994), S. 202-212 
    Details
    ISSN: 1615-6102
    Keywords: Fungal adhesion ; Appressoria ; Plant disease ; Cochliobolus heterostrophus ; Magnaporthe grisea ; Nectria haematococca ; Uromyces spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Firm adhesion of fungal plant pathogens to their hosts is critical at several stages in the host-parasite interaction. Spores of many fungal species are capable of rapid, non-specific attachment to various surfaces. This early adhesion, which often occurs well before germ tube emergence, prevents spores from being blown or washed from the host surface before infection can take place. Adhesion is critical for proper sensing of topographic signals involved in thigmotropic responses and for differentiation and function of appressoria. Four fungal pathogens which exhibit a variety of adhesion mechanisms have been selected for discussion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01666396
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  • 8
    Electronic Resource
    Electronic Resource
    Actin in penetration pegs of the fungal rice blast pathogen,Magnaporthe grisea (1992)
    Springer
    Protoplasma 168 (1992), S. 20-26 
    Details
    ISSN: 1615-6102
    Keywords: Appressoria ; Freeze substitution ; Immunofluorescence ; Plant disease ; Phalloidin ; Pyricularia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The penetration peg is the structure used byMagnaporthe grisea to pierce the surface of rice leaves or very hard nonbiodegradable substrates. Penetration pegs produced by appressoria in vitro were examined by electron microscopy and immunofluorescence microscopy using various fluorophore labeled anti-actins. Freeze-substitution preparation of appressoria at early stages of substrate penetration showed that peg cytoplasm consisted primarily of a zone of exclusion, excluding even ribosomes, and was continuous with a similar region in the appressorium. Apical vesicles were, however, observed in short, presumably elongating pegs. Immunofluorescence microscopy was used to demonstrate binding of a monoclonal anti-actin to penetration peg cytoplasm, following “permeabilization” of appressoria by means of a brief sonication. Occasional filaments and ca. 300 nm diameter plaques were labeled in appressorial cytoplasm. Western blot analysis of germ tube extracts showed that the monoclonal probe bound predominantly to a single band with a molecular weight similar to that of rabbit muscle actin. Preincubation of the antibody with actin virtually eliminated peg labeling. We conclude that the penetration peg contains actin which may play a role in the formation of the zone of exclusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01332647
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  • 9
    Electronic Resource
    Electronic Resource
    Phylogenetic relationships of Manihot species revealed by restriction fragment length polymorphism (1994)
    Springer
    Euphytica 76 (1994), S. 227-234 
    Details
    ISSN: 1573-5060
    Keywords: cassava ; Manihot ; RFLP ; phylogenetic relationships ; multilocus probes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two cloned cassava genes (pCAS6, pHNL) and a barley gene (pBLT63) which is highly conserved in higher plants, were evaluated as potential multilocus probes for RFLP analysis of phylogenetic relationships of species within the genus Manihot. For M. rubricaulis, M. chlorosticta and M. esculenta subsp. flabellifolia dendrograms based on 13 probe-restriction enzyme combinations show very little variation between accessions of the same species. A close relationship is demonstrated between the Mexican species M. chlorosticta and the M. esculenta subsp. flabellifolia accessions, which does not support the classification of flabellifolia as a true South American wild species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022167
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  • 10
    Electronic Resource
    Electronic Resource
    Effects of DNA charge and length on the electrophoretic mobility of intercalated DNA (1994)
    New York : Wiley-Blackwell
    Biopolymers 34 (1994), S. 1349-1358 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: DNA molecules ranging in size from 1 to 630 kilobase pair and intercalated with either ethidium bromide (EtBr) or propidium iodide (PI) were electrophoresed in 1% agarose at four different electric field strengths. The extent of intercalation of EtBr under the conditions of our electrophoresis experiments was determined by a spectroscopic technique, whereas the extent of intercalation of PI was inferred from previous studies. The effects of the increase in DNA contour length and the concomitant decrease of linear charge density were separated based on our analysis of the mobility data. We conclude that the main factor responsible for the reduced electrophoretic mobility of intercalated DNA is the diminished linear charge density and not the increased contour length. © 1994 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360341007
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