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  • 27.60.+j  (7)
  • Calcium
  • Immunocytochemistry
  • Springer  (17)
  • PANGAEA
  • 1990-1994  (17)
  • 1991  (17)
Collection
Publisher
  • Springer  (17)
  • PANGAEA
Years
  • 1990-1994  (17)
Year
  • 1
    ISSN: 1434-601X
    Keywords: 27.60.+j ; 23.20.−g ; 21.60.−n
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The properties of the triplet of low-lying states in101Mo have been studied through spectroscopy of theγ radiation following thermal neutron capture in100Mo and β− decay of101Nb and through a measurement of the proton angular distributions in the100Mo(d,p) reaction with 14 MeV deuteron energy. The half-lives of the 13.5 keV state and the 57.0 keV 5/2+ state have been measured as 226(7) and 133(7)ns, respectively. These values and the quadrupole/dipole mixing ratios of the 13.5 keV and 43.5 keV transitions yield spin and parity 3/2+ for the 13.5 keV level. The E2 components in the 13.5 (3/2+ →1/2+) and 43.5 keV (5/2+→3/2+) transitions are ≦ 8·10−4 and 54(9)%, respectively. The possibility of an additional state near to the 57.0 keV level is discussed. IBFM/PTQM calculations, taking into consideration the transitional character of the100Mo boson core, account for the electromagnetic-transition and transfer-reaction pattern of the triplet of states.
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  • 2
    ISSN: 1434-601X
    Keywords: 21.10.−k ; 23.20.Lv ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A non-yrast superdeformed band has been found in146Gd using the110Pd(40Ar, 4n)146Gd reaction. The nuclear assignment was made from the analysis of an experiment using a backed target. This band and the previously identified yrast SD band are probably not signature partners.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 340 (1991), S. 17-20 
    ISSN: 1434-601X
    Keywords: 25.40.EP ; 21.10.Ft ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The purpose of this work is to present calculations of the (p, n) charge exchange reactions which employ the density dependent interaction derived from the effective two-body interaction, and to study the reliability of a new expression of the nuclear density distribution. The results show that the theoretical framework of microscopic Lane model seems to be suitable for examining nuclear density distribution, and a reasonably good description of the (p, n) reaction can be given by using the new density distribution.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 340 (1991), S. 141-153 
    ISSN: 1434-601X
    Keywords: 21.10.Re ; 23.30.Lv ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excited states of73Se have been investigated up to spin, 21/2 using techniques of in-beamγ-ray spectroscopy in connection with the70Ge(α, n) reaction. Mean lifetimes of 12 levels have been determined applying Doppler-shift andγ-RF-methods. Five different bands have been identified that reflect a variety of different excitation modes. The decoupled 9/2+ band is likely to correspond to an oblate deformation while the 5/2+ band is interpreted as a strongly coupled prolate band built on the Nilsson configuration [422] 5/2+. The 3/2− band is a strongly coupled band built on the [301] 3/2} configuration.Nuclear reactions:70Ge(α,n),E=14, 16, 18, 19, 20MeV; measuredE γ,I γ,σ(E γ,θ),γγ-coin, linear polarization, DSA,γ(t).75Se deduced levels,I, π, τ, δ(E2/M1), B(σλ). Enriched targets, Ge detectors.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 88 (1991), S. 539-546 
    ISSN: 1432-1939
    Keywords: Wood mouse ; Bank vole ; Calcium ; Femur ; Ash
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calcium is essential for normal physiological function, reproduction and growth in mammals but its distribution in the natural environment is heterogeneous. Spatial variation in calcium soil content is especially marked in the Peak District, United Kingdom, where both calcium-rich limestone and calcium-poor gritstone rock types occur. Wood mice Apodemus sylvaticus (L) and bank voles Clethrionomys glareolus (Schreber 1780) from limestone areas had significantly higher calcium concentrations in stomach contents and in faeces compared with their counterparts from gritstone areas. Calcium status was assessed from serum calcium concentration, femur weight, ash content of the body, calcium concentration in the femur and body ash. There was no significant difference in serum calcium concentration, femur calcium concentration and body ash calcium concentration between animals from the limestone and the gritstone. However, on the limestone, bank voles, but not wood mice, had significantly heavier femora and a greater proportion of ash in the body compared with their gritstone counterparts.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 169 (1991), S. 39-50 
    ISSN: 1432-1351
    Keywords: Photoreception ; Retinally degenerate ; Mouse ; Circadian ; Rods ; Cones ; 11-cis retinaldehyde ; Immunocytochemistry ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We have examined the effects of light on circadian locomotor rhythms in retinally degenerate mice (C57BL/6J mice homozygous for the rd allele: rd/rd). The sensitivity of circadian photoreception in these mice was determined by varying the irradiance of a 15 min light pulse (515 nm) given at circadian time 16 and meauring the magnitude of the phase shift of the locomotor rhythm. Experiments were performed on animals 80 days of age. Despite the loss of visual photoreceptors in the rd/rd retina, animals showed circadian responses to light that were indistinguishable from mice with normal retinas (rd/+ and +/+). While no photoreceptor outersegments were identified in the retina of rd/rd animals (80–100 days of age), we did identify a small number of perikarya that were immunoreactive for cone opsins, and even fewer cells that contained rod opsin. Using HPLC, we demonstrated the presence and photoisomerization of the rhodopsin chromophore 11-cis retinaldehyde. The rd/rd retinas contained about 2% of 11-cis retinaldehyde found in +/+ retinas. We have yet to determine whether the opsin immunoreactive perikarya or some other unidentified cell type mediate circadian light detection in the rd/rd retina.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Planta 184 (1991), S. 67-73 
    ISSN: 1432-2048
    Keywords: Annexin ; Calcium ; Exocytosis ; Phospholipid-binding proteins ; Zea (annexins)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There is evidence that Ca2+ can regulate vesicle-mediated secretion in plant cells, but the mechanism for this is not known. One possibility is that Ca2+ -dependent phospholipid-binding proteins (annexins) couple the Ca2+ stimulus to the exocytotic response. Using a protocol developed for the isolation of animal annexins we have identified proteins in maize (Zea mays L.) coleoptiles that have similar characteristics to annexins. The predominant polypeptide species run as a doublet of relative molecular mass (Mr) 33000–35000 on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE); another less-abundant protein of Mr 23000 is also present. In the presence of Ca2+ these proteins bind to liposomes composed of acidic phospholipids. Calcium-sensitivity of binding differs for each protein and is also influenced by the pH of the buffer used for the liposome-binding assay. Antiserum raised to the 33 to 35-kDa doublet purified on SDS-PAGE recognises the doublet in crude extracts from maize and proteins of similar Mr in Tradescantia virginiana and tobacco Nicotiana tabacum L. The antiserum also recognises p68 (Annexin VI) from chicken gizzard extracts, indicating homology between animal annexins and the maize proteins. For the maize proteins to be involved in the regulation of exocytosis, binding to phospholipids would be expected to occur at physiological levels of Ca2+. The characteristics of the maize annexin-like proteins are described and attention drawn to the marked effect of pH in lowering the requirement for Ca2+ for phospholipid binding.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 168 (1991), S. 305-312 
    ISSN: 1432-1351
    Keywords: Blowfly ; Photoreceptor ; Receptor potential ; Mitochondrial activation ; Calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. Simultaneous measurements of the receptor potential and the light-induced mitochondrial activation were performed in white-eyed blowflies Calliphora vicina, mutant chalky, and Lucilia cuprina, mutants w F and w'nss. The intensity dependence and the temporal dynamics were investigated. 2. The characteristic curve of the light-induced mitochondrial activation vs. log intensity has an S-like shape, which is much steeper than the characteristic curve of the receptor potential (the V/log I curve). The threshold intensity of mitochondrial activation elicits about a half-maximal receptor potential, while mitochondrial activation and photoreceptor potential saturate at about the same intensity. 3. The time course of the mitochondrial activation induced by a light flash, i.e. the pulse-response curve, is biphasic in both mutants with normal phototransduction properties, Calliphora chalky and Lucilia w F ; the shape is slightly species dependent. 4. In the phototransduction mutant Lucilia w'nss the step-responses of both the (bright) light-induced mitochondrial activation and receptor potential are quite different from the corresponding signals in Lucilia w F . The striking resemblance of the step-response of the mitochondrial activation to its pulse-response indicates that mitochondrial activation and receptor potential are intimately linked.
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  • 9
    ISSN: 1432-1351
    Keywords: Aplysia ; Motoneurons ; Immunocytochemistry ; Small cardioactive peptides ; Facilitation ; Depression ; Buccal ; Feeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We used physiological recordings, intracellular dye injections and immunocytochemistry to further identify and characterize neurons in the buccal ganglia of Aplysia calif ornica expressing Small Cardioactive Peptide-like immunoreactivity (SCP-LI). Neurons were identified based upon soma size and position, input from premotor cells B4 and B5, axonal projections, muscle innervation patterns, and neuromuscular synaptic properties. SCP-LI was observed in several large ventral neurons including B6, B7, B9, B10, and B11, groups of s1 and s2 cluster cells, at least one cell located at a branch point of buccal nerve n2, and the previously characterized neurons B1, B2 and B15. B6, B7, B9, B10 and B11 are motoneurons to intrinsic muscles of the buccal mass, each displaying a unique innervation pattern and neuromuscular plasticity. Combined, these motoneurons innervate all major intrinsic buccal muscles (I1/I3, I2, I4, I5, I6). Correspondingly, SCP-LI processes were observed on all of these muscles. Innervation of multiple nonhomologous buccal muscles by individual motoneurons having extremely plastic neuromuscular synapses, represents a unique form of neuromuscular organization which is prevalent in this system. Our results show numerous SCPergic buccal motoneurons with widespread ganglionic processes and buccal muscle innervation, and support extensive use of SCPs in the control of feeding musculature.
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  • 10
    ISSN: 1432-0878
    Keywords: Rhabdomeres ; Cytoskeleton ; Actin ; Immunocytochemistry ; Membrane shedding ; Leptotarsus spp. (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rhabdomeres of tipulid flies lose membrane during turnover from a ‘shedding zone’ composed of microvillar tips. These distal domains lack intramicrovillar cytoskeletons and appear to be empty sacs of membrane. Recent concerns about the role of ninaC mechano-enzymes in the architecture of dipteran rhabodomeral microvilli and the dynamic role that they may play in the creation of shedding zones demand an examination of the distribution of actin in tipulid rhabdomeres. We compared rhabdomeres from tipulid retinae incubated before fixation for immunocytochemistry in a buffer without additives and a stabilising buffer that contained a cocktail of cysteine protease inhibitors; both were challenged by an anti-actin antibody for immunogold labelling after embedding in LR White Resin. Shedding zones thus processed collapse to structureless detritus. Stabilised and unstabilised shedding zones were immunonegative to anti-actin. To ensure that the negative results were not consequent upon conformational changes generated by the processing protocol, we examined microvilli of degenerating rhabdomeres of the Drosophila light-dependent retinal degeneration mutant rdgB KS222 (which separate and collapse without creating a shedding zone) and found the detritus they generate to be immunopositive to anti-actin. Stabilised and unstabilised regions of basal regions of tipulid rhabdomeres were equally immunopositive. We infer that (a) actin is absent from shedding zones; (b) actin is not degraded by microvillar cysteine proteases. The implications of these conclusions are discussed in relation to some functional models of arthropod photoreceptor microvilli.
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