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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of environmental contamination and toxicology 19 (1990), S. 704-711 
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract Concentrations of four essential elements (Fe, Mn, Zn, and Cu) and two toxic metals (Cd and Hg) were measured in selected tissues of 19 pelagic seabird species collected in the North Pacific and neighboring waters. Essential metal concentrations were generally highest in the liver and less variable than toxic metals among species and also within each species. Fe concentrations in the muscle were higher in Alcidae than in the other families, whereas the opposite trend was found for Fe and Mn in the liver. Zn concentrations varied among species, depending on the Cd concentrations. On the other hand, toxic metal concentrations were highest in the liver or kidney and varied widely among species, greatly depending on differences in the diet among species. Extraordinarily high Hg concentrations were found in Black-footed Albatrosses,Diomedea nigripes, exceeding 300 μg/g wet weight in some, and seemed to be due to constraints on the elimination of Hg. Also, some geographical differences in Cd and Hg concentrations of the seabirds were observed. The concentrations of Cd and Hg reported here, however, seem to be natural rather than due to environmental pollution.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of thermal analysis and calorimetry 38 (1992), S. 1939-1945 
    ISSN: 1572-8943
    Keywords: enthalpy of phase transition ; iron Chevrel phase sulphide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Nach der Tropfenmethode wurden mittels eines Hochtemperaturdoppelkalorimeters vom Typ Calvet im Temperaturbereich 300–500 K an Fe2Mo6S7.8Messungen der Enthalpieinkremente HT-H298K durchgeführt. Die Phasenumwandlung erster Ordnung bei diesem Sulfid von der triklinen (Niedertemperaturphase) in die rhomboedrische (Hoch-temperaturphase) erfolgt bei 375 K und die Enthalpie erhielt man mit einem Wert von 6.0 kJ/mol. Die Wärmekapazitäten für Fe2Mo6S7.8 wurden sowohl vor als auch nach der Phasenumwandlung berechnet.
    Notes: Abstract Enthalpy increment HT-H289K measurements have been made on iron Chevrel phase sulphide Fe2Mo6S7.8, in the temperature range 300 to 500 K by the drop method using a hightemperature Calvet-type twin calorimeter. The first-order phase transition of this sulphide from a triclinic (low-temperature phase) to a rhombohedral (high-temperature phase) occurred at 375 K, and the enthalpy was evaluated to be 6.0 kJ/mol. The heat capacities of iron Chevrel phase sulphide Fe2Mo6S7.8 were also calculated before and after the phase transition.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Oxidation of metals 38 (1992), S. 347-363 
    ISSN: 1573-4889
    Keywords: SUS430 stainless steel ; abnormal oxidation ; moist atmosphere ; incubation period
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The oxidation behavior of SUS430 stainless steel was studied in moist atmospheres $$(P_{H_2 O} = 3.4 \times 10^3 Pa, P_{O_2 } = 20 \sim 12.6 \times 10^3 Pa)$$ at 873 K. The mass gain during oxidation was measured by using a thermobalance. An abnormal rapid oxidation occurred after an incubation period which increased with increasing oxygen partial pressure. The product on the surface was identified as α-Fe2O3 of whisker-like morphology by X-ray diffraction and scanning electron microscopy. The abnormal oxidation was not observed under high-oxygen partial pressures. The results of the experiments conducted in various atmospheres were analyzed and are discussed.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 84 (1992), S. 33-38 
    ISSN: 1432-2242
    Keywords: Homologous recombination ; ATPase α-subunit ; Protoplast fusion ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Segments of mitochondrial DNA (mtDNA) carrying the gene for the α-subunit of F1-ATPase (atpA) were detected by Southern hybridization with atpA from pea as probe. In the case of Nicotiana langsdorffii, we identified four fragments that are derived from combinations of two different 5′ and two different 3′ flanking regions of atpA. All four types share the coding region, suggesting that they result from homologous recombination in the coding region of atpA. By contrast, N. glauca generated only one analogous fragment, which indicated the existence of only a single type of atpA in N. glauca. In the case of somatic hybrids obtained by fusion between protoplasts from N. langsdorffii and N. glauca, analysis with EcoRI or HindIII detected three new fragments in addition to the parental fragments. These new fragments can be explained by homologous recombination within the coding region of atpA. Our results show that the coding region of atpA is involved not only in intragenomic homologous recombination but can also be involved in homologous recombination between two parental mitochondrial genomes of somatic hybrids.
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  • 5
    ISSN: 1432-0878
    Keywords: Atrial natriuretic peptides ; mRNA ; Diabetes, type I ; Immunohistochemistry ; Morphometry ; Mouse (NOD)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Atrial natriuretic peptide (ANP) levels in cardiocytes and plasma were examined by using immunohistochemistry, electron microscopy, and radioimmunoassay in non-obese diabetic mice (NOD). Cardiocyte ANP mRNA expression was measured by the polymerase chain reaction method. ANP immunoreactivity in the auricular cardiocytes was more prominent in hyperglycemic mice (NOD-h) than in normoglycemic mice (NOD-n). Ultrastructural examination showed that auricular cardiocytes of the NOD-h group contained more cytoplasmic granules than cells of the NOD-n group. Ultrastructural morphometry indicated that the number of granules per auricular cardiocyte was significantly larger in the NOD-h group than in the NOD-n group. (P〈0.01), whereas the granule diameter was significantly smaller in the NOD-h group (P〈0.01). Radioimmunoassay showed that ANP levels in the NOD-h auricular cardiocytes were significantly higher than those in the NOD-n cardiocytes (P〈0.01); the opposite was true in plasma. Cardiocyte ANP mRNA expression was lower in the NOD-h group than in the NOD-n group.
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  • 6
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Mössbauer, magnetic susceptibility and electron paramagnetic resonance (EPR) studies of inactive and photoactivated NHase enzymes were performed to elucidate the electronic change of non-heme two-iron atom center of the enzyme by photoactivation. These spectroscopic investigations revealed that both the two iron atoms of the active NHase could be assigned to low-spin ferric state, and those of the inactive NHase could each be assigned to low-spin ferric and low-spin ferrous ones. From these results, it was concluded that one of the non-heme iron atoms is oxidized in the inactive NHase during photoactivation.
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  • 7
    ISSN: 1573-0778
    Keywords: bispecific monoclonal antibody ; human hybrid hybridoma ; immunotoxin ; Pseudomonas aeruginosa exotoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract By fusing a human hybridoma producing an IgG2ķ antibody against human A431 epidermoid carcinoma cells with an Epstein-Barr virus-transformed human B lymphocyte producing an IgG2ķ antibody against Pseudomonas aeruginosa exotoxin A, we established a hybrid hybridoma producing a bispecific monoclonal antibody reacting with both A431 cells and the exotoxin. Human IgG was purified from the culture supernatant of the hybrid hybridoma, and the bispecific monoclonal antibody in the IgG preparation was further separated from the two parental antibodies by hydroxyapatite high-performance liquid chromatography. The human bispecific monoclonal antibody thus obtained efficiently targeted the antibody-reative cells, A431, for attack by the exotoxin in vitro.
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  • 8
    ISSN: 1573-0875
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Aqueous solutions of various amino acids were irradiated with60Co-γ-rays, and subsequently the remaining amino acids were analyzed using HPLC. The D37 for the 1 mM glycine and alanine solutions were 1.95×104 and 1.48×104 Gy, respectively. However, when the mixed solutions of glycine and alanine (each in 0.5 mM) were irradiated under the identical condition, the D37 for the glycine decomposition increased to 3.56×104 Gy, while that for alanine decreased to 0.65×104 Gy. A similar phenomenon was observed also in the case of the mixed solutions of aspartic acid and alanine. Namely, aspartic acid was protected from the attack of radiation by the presence of alanine in the solutions. The most interesting finding in this combination experiment is that, when D,L-aspartic acid was irradiated in the presence of L-alanine, the radiation-sensitivity of L-aspartic acid decreased selectively and vice versa. Namely, the asymmetric field induced in the solutions by adding D- or L-alanine might affect the radiodecomposition rate of either aspartic acid. Addition of glycine to D,L-aspartic acid did not bring about the asymmetric decomposition. It seems that some interaction between these amino acid molecules resulted in this effect.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Origins of life and evolution of the biospheres 21 (1992), S. 243-249 
    ISSN: 1573-0875
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract We prepared thermal heterocopolymers of amino acids, aspartic acid and proline, and solubilized them in distilled water at boiling temperature. The resulting suspension was then cooled down while controlling the cooling rate. The maximum growth of phase-separated microspheres was found to occur at a finite, nonzero cooling rate. Temperature-gradient controlled growth of these microspheres indicates that aggregation of thermal heterocopolymers of amino acids in their aqueous milieu is irreversible. In view of the fact that evolutionary process is an indication of lasting physical irreversibility, thermal heterocopolymers of amino acids were shown to have an evolutionary significance in maintaining the capacity of irreversibility lasting over at least several hours.
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  • 10
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Kinases of the mammalian cdc2 family including cdk2 (cyclin-dependent kinase 2) are thought to be involved in both the G2/M transition and DNA replication. To investigate the role of cdc2 kinase and cdk2 in cell cycle progression, murine tsFT210 cells bearing a temperaturesensitive cdc2 mutation were used. These kinases were purified by column chromatography, using a peptide with the consensus phosphorylation site of cdc2 kinase as the substrate. In this mutant, cdc2 kinase activity was temperature sensitive and cdk2 activity was not. At the restrictive temperature, the mutant was only arrested in the G2 phase and not in the G2-S phase, suggesting that cdk2 did not compensate for cdc2 kinase at the G2/M transition but did function at the G1-S phase. This suggestion was supported by the finding that transfection of cdk2 cDNA did not improve the growth of the mutant cell line at the restrictive temperature, although transfection of cdc2 cDNA did.
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