ISSN:
0935-6304
Keywords:
Gas chromatography
;
Capillary columns, glass
;
XE-60-L-valine-(S)-α-phenylethylamide
;
Amino acid derivatization, general procedure
;
N-Pivaloyl amino acid esters
;
Amino acid enantiomer separation
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A laboratory working procedure is described for silanization of glass capillaries with bis(cyanopropyl)tetramethyldisiloxane, prior to coating with XE-60-L-valine-(S)-α-phenylethylamide as chiral stationary phase. GC columns thus prepared exhibit good inertness and no long-term deterioriation of enantioselectivity up to 220°C. A general procedure is given for derivatization of simple and of polyfunctional amino acids, in a batch mixture, simultaneously, and completely at both the N- and the C-terminus and all other protic functions. Within the seven series of N-pivaloyl amino acid esters tested (methyl to neopentyl), baseline separation is obtained for all R/S pairs, including the proline derivative.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240120405
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