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Reduced variation in the clonogenic assay obtained by standardization of the cell culture conditions prior to drug testing on human small cell lung cancer cell lines (1989)

Jensen, Peter Buhl ; Roed, Henrik ; Vindeløv, Lars ; [et al.]

Springer

Investigational new drugs 7 (1989), S. 307-315

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ISSN: 1573-0646

Keywords: interexperimental variation ; in vitro chemosensitivity ; small cell carcinoma of the lung ; anthracycline analogues

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology , Medicine

Notes: Summary An advantage of established tumor cell lines compared to fresh human tumor specimens used in sensitivity assessments is the possibility of repeated experiments. Ultimately a database of sensitivity profiles on a panel of cell lines can be made and the sensitivity to new drugs compared with historical data. A prerequisite of this strategy is a minimal interexperimental variation. The sensitivity of eight human small cell lung cancer cell lines to adriamycin, daunomycin, aclacinomycin A, and mitoxantrone was tested in the clonogenic assay. A covariation in the sensitivity to the drugs emphasized the importance of simultaneous drug testing on the same batch of cells. On one cell line (NCI-N592) the interexperimental variation was further evaluated and a significant correlation was found between preexposure culture conditions, size of S-phase, and sensitivity to adriamycin, daunomycin, and mitoxantrone. Rigorous standardization of the growth conditions prior to clonogenic assay reduced the variation in the sensitivity to adriamycin from a factor of five to only 10–15%. It is concluded that simultaneous experiments on the same batch of cells in drug comparisons should be used if possible. Specification and standardization of culture conditions are necessary in the comparison of drugs tested in different experiments. Inclusion of the same reference drug in all experiments may further increase the validity of comparisons in different experiments.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00173760

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Pseudochalkogenverbindungen. XVIIIXVII. Mitt. siehe Lit. [12]. Zur Cyanamidolyse von Organophosphoryl-(phosphonyl-)chloriden (1989)

Köhler, H. ; Kretschmann, M. ; Jäger, L. ; [et al.]

Weinheim : Wiley-Blackwell

Zeitschrift für anorganische Chemie 568 (1989), S. 35-48

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ISSN: 0044-2313

Keywords: Chemistry ; Inorganic Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: Pseudochalcogen Compounds. XVIII. Cyanamidolysis of Organophosphoryl-(phosphonyl-) chloridesCyanamidolytic reactions of organophosphoryl- and -phosphonyl chlorides are leading in dependence on the conditions of the reactions to metal-organo-cyanamidophosphates, M[(RO)2P(Y)NCN], and -cyanamidophosphonates, M[(RO)R'P(Y)NCN], M2[RP(Y)(NCN)2] respectively, as well as to the corresponding organo phosphoryl (phosphonyl) hydrogencyanamides, (RO)2P(Y)NHCN, (RO)R'P(Y)NHCN, RP(Y)(NHCN)2 and to Bis-(organophosphoryl)- and Bis-(organophosphonyl)-carbodiimides, (RO)2P(Y)-NCN-P(Y)(OR)2, (RO)R'P(Y)-NCN-P(Y)R' (OR). (Y: O, S). Conditions for synthesis as well as properties and structures of the new compounds are discussed on the basis of characteristic IR- and 31P-NMR-data.

Notes: Cyanamidolysereaktionen an Organophosphoryl- und -phosphonyl-chloriden führen in Abhängigkeit von den gewählten Reaktionsbedingungen zu Metall-organo-cyanamidophosphaten, M[(RO)2P(Y)NCN], Metall-organo-cyanamidophosphonaten, M[(RO)R'P(Y)NCN], M2[RP(Y)(NCN)2] (Y: O, S), ferner zu den entsprechenden Organophosphoryl-(phosphonyl)-hydrogencyanamiden, (RO)2P(Y)NHCN, (RO)R'P(Y)NHCN, RP(Y)(NHCN)2 sowie zu Bis-(organophosphoryl)-carbodiimiden, (RO)2P(Y)—NCN—P(Y)(OR)2 und Bis-(organophosphonyl)-carbodiimiden, (RO)R'P(Y)-NCN-P(Y)R'(OR). Synthesebedingungen, Strukturen und Eigenschaften der neuen Verbindungen werden anhand der charakteristischen IR- und 31P-NMR-Daten diskutiert.

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