ISSN:
1617-4623
Keywords:
Spontaneous mutation
;
Spectrum
;
Human hprt cDNA
;
pZip shuttle
;
Misalignment mutagenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Altered sequences were determined of 52 independent spontaneous mutations occuring in a cDNA of the human hypoxanthine phosphoribosyltransferase (hprt) gene, which was integrated into chromosomal DNA of the mouse cell as a part of the retroviral shuttle vector. Spontaneous mutations comprised a variety of events: base substitutions, frameshifts, deletions, duplications, and complex mutational events, and were distributed randomly over the coding region of the gene. Frameshifts were the most frequent mutational event (38%), and base substitutions were the next most frequent (25%), followed by deletions (19%). Frameshift and deletion mutations commonly occurred preferentially at sites flanked by short direct repeats. Short inverted repeats were frequently found to be associated with duplication and complex mutational events. Analysis of the sequence alterations in the mutant genes suggests that misalignment mutagenesis represents an important molecular mechanism for the generation of spontaneous mutations in eukaryotic cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00259606
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