ISSN:
1573-5028
Keywords:
Nicotiana
;
promoter
;
self-incompatibility
;
S-RNase
;
transgenic
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Nicotiana tabacum and Nicotiana alata plants were transformed with genomic clones of two S-RNase alleles from N. alata. Neither the S 2 clone, with 1.6 kb of 5′ sequence, nor the S 6 clone, with 2.8 kb of 5′ sequence, were expressed at detectable levels in transgenic N. tabacum plants. In N. alata, expression of the S 2 clone was not detected, however the S 6 clone was expressed (at low levels) in three out of four transgenic plants. An S 6-promoter-GUS fusion gene was also expressed in transgenic N. alata but not N. tabacum. Although endogenous S-RNase genes are expressed exclusively in floral pistils, the GUS fusion was expressed in both styles and leaves.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00042080
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