ISSN:
0749-503X
Keywords:
Brettanomyces
;
custers effect
;
glycosis
;
organic hydrogen acceptors
;
mass spectrometry
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The yeast Brettanomyces anomalus showed the Custers effect in that under strictly anaerobic conditions, in the presence of glucose, CO2 production was negligble. CO2 production was stimulated by mixing anaerobic cell suspensions with an aerated glucose solution in astopped-flow cell. Glycolytic CO2 production continued even after oxygen exhaustion. Studies using an open reaction vessel showed that the rate of glycolytic CO2 production could be increased to a maximum level by exposing the anaerobic cell suspension to brief pulses of O2. A cell suspension CO2 at a maximal rate demonstrated the Pasteur effect on switching the mobile gas to a mixture conatining oxygen (5.05 KPa). In contrast to glycolytic CO2 production in vivo nicotinamide pool responded rapidly to changes in oxygen concentration. The addition of acetaldehyde, acetone, or 3-hydroxy-butan-2-one led to a temprorary production of CO2 at an initial rate depending on the concentration of substance added according to the Michaelis-Menten equation. The maximal rates were equal with all three substances, whereas tha apparent Km values were different. The total amount of CO2 produced was 22-fold greater than the amount of acetaldehyde added. Added organic hydrogen acceptors modulated the intracellular reedox balance of B. anomalus under conditions. These results are discussed in relation to the current hypothesis of the Custers effect.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/yea.320040403
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