ISSN:
0749-1581
Keywords:
RELAY 2D NMR
;
1H NMR
;
2D NMR Steroids
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Relayed coherence transfer (RELAY) two-dimensional NMR spectroscopy was demonstrated to be an effective technique for overcoming the problem of overlapping multiplets in the analysis of the 1H NMR spectra of steroids. Cross-sections through appropriate proton resonances resulted in resolved spectra of the spin systems for each of the saturated rings of the estrogenic hormones 17β- and 17α-estradiol. During RELAY experiments with a fixed delay time of 0.042 s it was possible to observe RELAY cross-peaks between H-11α and 18-CH3 which resulted from magnetization transfer via long-range coupling between 18-CH3 and H-12α. The difference in stereochemistry at C-17 of these estradiol epimers allowed an examination of the effect of substituent configuration on the RELAY cross-peak intensity in order to determine the ability of the RELAY method to provide qualitative structural information.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260260308
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