ISSN:
1617-4623
Keywords:
Restriction fragment length polymorphisms
;
Gossypium hirsutum
;
Gene copy number
;
Lea, vicilin, and legumin genes
;
Tetraploidization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have determined the copy number of 21 genes in an allotetraploid and several diploid species of cotton by gel and dot blot hybridization with cloned cDNAs. The legumin A, legumin B, and all 18 unique Lea (late embryogenesis-abundant) cDNA sequences isolated from the AD allotetraploid Gossypium hirsutum are present in one copy in A, D, E, and F diploid species and in two copies in G. hirsutum. Gel blot analysis of DNAs digested with EcoRI or BamHI usually detects different sized fragments in A and D diploids. Conservation of these restriction fragment length polymorphisms in G. hirsutum allows most of these fragments to be assigned to their respective subgenomes. Furthermore, both subgenomes in G. hirsutum can be distinguished from those in the interfertile allotetraploid G. barbadense. These results show that physical mapping of both sets of chromosomes in an allotetraploid should be possible by segregation analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330608
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