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Third chromosome suppressor of position-effect variegation loci in Drosophila melanogaster (1986)

Reuter, G. ; Dorn, R. ; Wustmann, G. ; [et al.]

Springer

Molecular genetics and genomics 202 (1986), S. 481-487

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ISSN: 1617-4623

Keywords: Position-effect variegation ; Suppressor mutations ; Chromosome 3 ; Heterochromatin ; Drosophila melanogaster

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary As a result of a genetic analysis of 63 third chromosome suppressor mutations of position-effect variegation 12 different loci showing dominant suppression have been identified and their map positions determined. A compilcation of the genetic data available for each suppressor locus is given. The strong suppressor effects of the mutations have been quantified by measurements of white variegation inw m4h /w m4h ,w m4h /Y andw m4h /O flies. Mutant alleles of three loci were found in these studies to dominate over the strong enhancer effect of complete loss of the Y chromosome. Most of the identified loci suppressing position-effect variegation represent essential genetic funtions; only three loci represent nonessential functions. Mutations of two loci display recessive butyrate sensitivity and lethal interaction with the heterochromatic Y chromosome suggesting that these genes affect chromosomal condensation. Studies with deficiencies and triploids revealed that most of the loci represent haplo-abnormal suppressor functions. The use of the isolated mutant material for genetic, developmental and molecular studies of processes connected with gene inactivation in position-effect variegation is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00333281

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Sponge aggregation factor: In situ localization by fluorescent monoclonal antibody techniques (1986)

Gramzow, Monika ; Dorn, August ; Steffen, Renate ; [et al.]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 31 (1986), S. 251-258

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ISSN: 0730-2312

Keywords: aggregation factor ; monoclonal antibodies ; reaggregation ; cell recognition ; Geodia cydonium ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: The aggregation factor (AF) from sponges mediates a heterophilic interaction of homologous cells. Applying electron microscopical means, we succeeded only very rarely in identifying the 90 S AF particle in tissue sections from Geodia cydonium. By means of a fluorescent antibody technique, we have now localized the cell binding domain of the AF in situ. Previous studies in this laboratory have led to the identification of the 47-kDa cell binding protein of the AF, using the monoclonal antibody (mab) 5D2-D11 [Gramzow M, Bachmann M, Zahn RK, Uhlenbruck G, Dorn A, Müller WEG, J Cell Biol, 102:1344-1349, 1986]. This mab and mab 7D5, directed against a 92-kDa protein in the AF complex, were chosen for the fluorescent studies. By using mab 5D2-D11, the plasma membranes of cells from different regions in the sponge could be brightly stained. However, mab 7D5 reacted only very weakly with the sponge surfaces. By applying the immuno-blotting technique it was furthermore demonstrated that the cell binding protein is present both in the associated form with AF complex and in a free state. Moreover, it was established that the 47-kDa binding protein is not present in (1) homologous glycoconjugates, (2) lectin, or (3) collagen; these components are known to be involved in cell-matrix interaction.

Additional Material: 5 Ill.