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  • Chemistry  (546)
  • Polymer and Materials Science  (232)
  • ASTROPHYSICS  (229)
  • Life and Medical Sciences  (118)
  • Humans  (82)
  • SPACE RADIATION
  • 1985-1989  (1,000)
  • 1986  (1,000)
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Keywords
Publisher
Years
  • 1985-1989  (1,000)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Differential expression of metastasis-associated cell surface glycoproteins and mRNA in a murine large cell lymphoma (1986)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 31 (1986), S. 305-312 
    Details
    ISSN: 0730-2312
    Keywords: tumor metastasis ; gene expression ; oncogenes ; virus antigens ; glycoproteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A metastatic variant cell subline of the Abelson virus-transformed murine large lymphoma/lymphosarcoma RAW 117 has been selected in vivo ten times for liver colonization. Highly metastatic subline RAW117-H10 forms greater than 200 times as many gross surface liver tumor nodules as the parental line RAW117-P. Analysis of cellular proteins and glycoproteins indicates reduced expression of murine Moloney leukemia virus-associated p15, p30, and gp70, and increased expression of a sialoglycoprotein, gp150, in the highly metastatic H10 cells. Northern analyses of oncogene expression suggested that mRNA of various oncogenes was expressed equally or not expressed in the RAW117 cells of differing metastatic potential. Differential gene expression was examined using a cDNA library of 17,600 clones established from poly A+ mRNA isolated from H10 cells. The cDNA library was screened by the colony hybridization technique using probes made from both RAW117-P and -H10 cells. Approximately 99.5% of these cDNA clones were expressed identically in P and H10 cells. Of the few differentially expressed cDNA clones (approx. 150/17,600), one-half of these were identified as Moloney leukemia virus sequences in a separate probing with a radiolabeled Moloney leukemia virus probe. The remainder of the differentially expressed mRNA detected by colony hybridization of the cDNA library were expressed at higher levels (approx. 1/6) or lower levels (approx. 1/3) in the highly metastatic H10 cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240310408
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of trichroic infrared absorption in solids. III. Phase I poly(vinylidene fluride) (1986)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 24 (1986), S. 2541-2551 
    Details
    ISSN: 0887-6266
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Trichroic infrared absorption has been measured for oriented phase I poly(vinylidene fluoride). In addition to intensity differences, frequency shifts in the plane transverse to the draw direction are apparent. Shifts up to 10 cm-1 in one-way drawn phase I are found for the CF2 stretching vibration (880 cm-1) between the direction normal to the film surface (thickness direction) and the remaining directions. Annealing the highly oriented films produces no major changes in the unique three-dimensional structure. A model based on crystal defect structures is proposed to explain the unusual frequency shift behavior in the phase I PVDF. Possible conformations and a mechanism of formation of the defects are discussed.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/polb.1986.090241111
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  • 3
    Electronic Resource
    Electronic Resource
    Partial characterization of a major gut thiol proteinase from larvae of Callosobruchus maculatus F. (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 3 (1986), S. 561-575 
    Details
    ISSN: 0739-4462
    Keywords: Callosobruchus maculatus ; proteinase inhibitors ; thiol digestive proteinase ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Much of the proteolytic activity in the digestive tract of Callosobruchus maculatus larvae can be attributed to a thiol proteinase(s) that hydrolyzes [3H]methemoglobin optimally at pH 5.0. Maximal hydrolysis of [3H]methemoglobin, [3H]alpha-casein, and N-benzoyl-DL-arginine napthylamide-(BANA) required the presence of thiol reducing agents. Larval gut proteinase activity was strongly inhibited by p-hydroxymercuribenzoic acid (pHMB), Nethylmaleimide (NEM), and iodoacetic acid (IAA) but was unaffected by the Bowman-Birk and Kunitz proteinase inhibitors from soybeans or by lima bean trypsin inhibitor. L-Trans-epoxysuccinyl-leucylamido-(4-guanidino)-butane (E-64), a specific inhibitor of thiol proteinases, potently inhibited proteolysis of [3H]methemoglobin by larval gut homogenates. Proteolytic activity in the larval gut was located in the lumen contents and thus appears to play a major role in extracellular digestion. The pH of the larval midgut is slightly acidic, and midgut contents exhibit a negative redox potential, conditions supporting the activity of a thiol proteinase. The significance of these findings is discussed with reference to the vulnerability of this digestive proteinase as a target for existing or genetically engineered plant chemical defenses.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/arch.940030607
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  • 4
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    Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-08-22
    Description: A new family of protein kinase C-related genes has been identified in bovine, human, and rat genomes. The alpha-, beta-, and gamma-type protein kinase sequences are highly homologous, include a kinase domain, and potential calcium-binding sites, and they contain interspersed variable regions. The corresponding genes are located on distinct human chromosomes; the possibility of even greater genetic complexity of this gene family is suggested by Northern and Southern hybridization analyses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Coussens, L -- Parker, P J -- Rhee, L -- Yang-Feng, T L -- Chen, E -- Waterfield, M D -- Francke, U -- Ullrich, A -- New York, N.Y. -- Science. 1986 Aug 22;233(4766):859-66.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3755548" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Base Sequence ; Cattle ; Chromosome Mapping ; Chromosomes, Human, 16-18 ; Dna ; Genes ; Humans ; Nucleic Acid Hybridization ; Protein Kinase C/*genetics ; Rats
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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    PAPER CURRENT
  • 5
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    Occultation studies of the solar system (1986)
    In:  CASI
    Details
    Publication Date: 2006-02-14
    Description: Determination of the size, shape, mean density, and albedo of Ceres was made. Predictions for 133 occultations of bright stars occurring in 1986 and 1987 were completed and published, as have predictions of occultations of stars by Comet Halley. Twenty-nine mutual events involving Galilean satellites were observed at Flagstaff in 1985.
    Keywords: ASTROPHYSICS
    Type: NASA, Washington Reports of Planetary Astronomy, 1985; p 108-109
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    The molecular gas in the supernova remnant IC443 (1986)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: Although a few highly perturbed regions characterized by gas motions with velocities larger than 20 km/s have been discovered during the last several years in the supernova remnant (SNR) IC 443, the nature of these perturbed clumps and their relationship to the quiescent molecular gas near the SNR remains unknown. In part, this is due to a lack of large-scale, high angular resolution observations. Therefore, a systematic survey of this SNR in the CO (J=1 yields 0) line has been conducted, covering a roughly 50' x 50' region spaced by 2'. The observations were made with the 14 m telescope of the Five College Radio Astronomy Observatory (FCRAO), which has a resolution of 45" and a single sideband receiver temperature of 200 K at 2.6 mm wavelength. Five new clumps were discovered, bringing the total number of known perturbed regions to eight. To study the physical structure of these clumps in more detail, more complete maps of the clumps have been made in both the CO(J=1 yields 0) and (J=2 yields 1) transitions with the FCRAO telescope. These maps show that the extent of perturbed gas in a typical clump is several arcmin, or a few pc at a distance of 1.5 kpc.
    Keywords: ASTROPHYSICS
    Type: NASA. Ames Research Center Summer School on Interstellar Processes: Abstracts of Contributed Papers; p 69-70
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 7
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    A molecular cloud in the local, hot interstellar medium (1986)
    In:  Other Sources
    Details
    Publication Date: 2019-07-12
    Description: Echelle spectra recorded at the D lines of Na I are reported for nine A or F stars. Lying at approximate distances ranging from 25 to 230 pc, the stars are projected on or near the high-latitude molecular cloud MBM 12 at l = 159 deg, b = -34 deg. Among a subgroup of five of these stars separated by no more than 1.2 deg on the sky, four which are located at distances d more than 70 pc show strong interstellar D line absorption near the radial velocity of the CO emission observed in this general direction. The fifth star, at roughly 60 pc, shows no detectable absorption. MBM 12 therefore probably lies at roughly 65 pc, within the local region filled primarily by very hot, low-density gas, a conclusion supported by the large internal velocity dispersion of the molecular cloud complex.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 2 - Letters to the Editor (ISSN 0004-637X); 306; L109-L11
    Format: text
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    NASA TECHNICAL REPORTS
  • 8
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    Infrared cirrus and high-latitude molecular clouds (1986)
    In:  Other Sources
    Details
    Publication Date: 2019-07-12
    Description: It is established that a close correlation exists between far-infrared cirrus emission observed with IRAS and the CO emission from high-latitude molecular clouds (HCLs). In all cases, the HLCs correspond to the central portions of 100-micron infrared cirrus features. This association firmly establishes at least some of the cirrus as features of the local interstellar medium with typical distances of 100 pc. The infrared energy distribution of the cirrus displays an excess of 12-micron and 25-micron emission over that expected from dust at equilibrium temperature, consistent with emission from very small (less than 10 A) transiently heated grains.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 2 - Letters to the Editor (ISSN 0004-637X); 306; L101-L10
    Format: text
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    NASA TECHNICAL REPORTS
  • 9
    Electronic Resource
    Electronic Resource
    Specificity in DNA interactions: An nmr investigation of the interaction of propidium with oligodeoxyribonucleotides containing normal and G · T base pairs (1986)
    New York : Wiley-Blackwell
    Biopolymers 25 (1986), S. 1997-2015 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The interaction of propidium with three self-complementary oligodeoxyribonucleotides has been investigated by 1H- (base-pair imino proton assigned by 1D NOE and saturation transfer methods) and 31P-nmr as a function of ratio of propidium to oligomer (from zero to saturation) and temperature. The three oligomers are dTATATGCGCATATA (1), dTATATGTGCATATA (2), which has the same sequence as 1 except for the mismatched base pair at position 7, and dTATGTGCATA (3), which is a shortened version of 2. The imino proton chemical-shift changes of 1 on titration with propidium can be explained by the effects of the ring-current anisotropy of propidium at intercalation (3.4 Å) and next-neighbor sites (6.8 Å). The results indicate that propidium binds with neighbor exclusion but with no significant specificity for any intercalation site in the sequence of 1. The addition of propidium to 1 results in general downfield shifts of all 31P signals, as expected for a nonspecific intercalator. Imino and 31P-nmr spectra for 2 indicate that this oligomer forms a hydrogen-bonded G · T base pair at position 7 with little change in base pairing and stacking of base pairs 1-6 compared to 1. The results for addition of propidium to 2 and 3 are quite different than with 1. At low ratio only secondary shifts (6.8 Å) are seen for the G and T imino protons of base-pair 7 on addition of propidium. At higher ratios of propidium, the signals for these G and T protons are lost in 2 and severely broadened in 3, even at low temperature. The other potential intercalation sites in 2 and 3 appear to bind propidium strongly and without significant specificity as with 1. 31P spectra of 2 in the presence of propidium show the expected downfield shifts and broadening. Thus, the minor differences in local helix geometry in 1, and in 2 and 3, away from the G · T base pair do not significantly affect propidium intercalation specificity. Having one or two G · T base pairs at a site, however, makes intercalation in the standard manner significantly less favorable.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360251013
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  • 10
    Electronic Resource
    Electronic Resource
    Synthesis of a Versatile Prostaglandin Intermediate by Ozonization of endo-cis-Bicyclo[3.3.0]oct-6-en-2-olDedicated to Prof. Dr. habil. Helmut Dorn on the occasion of his 60th birthday (1986)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 328 (1986), S. 430-434 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The ozonization of endo-cis-bicyclo[3.3.0]oct-6-en-2-ol (1) in methanol followed by reduction with dimethyl sulfide in the presence of traces of an acid yields in a one-pot procedure an anomeric mixture of 3-exo-methoxy- and 3-endo-methoxy-2-oxabicyclo[3.3.0]octane-6-endo-carbaldehyde (3a and 3b) in a ratio of 9:1. This mixture is converted by base catalyzed epimerization in an overall yield of about 65% into a mixture of the anomeric 6-exo-aldehydes 5a and 5b, a versatile starting material for the synthesis of prostaglandins of the PG2-type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19863280319
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