ISSN:
1573-5028
Keywords:
Agrobacterium rhizogenes
;
Solanum tuberosum
;
T-DNA
;
gene expression
;
tuberisation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Expression of TL-DNA from Agrobacterium rhizogenes plasmid pRi 1855 was examined in a transformed derivative of Solanum tuberosum cv. Desiree, D9X8a. Northern blot analysis identified at least nine TL-DNA coded transcripts in roots, shoots and tubers but their relative abundance differed within and between organs. This revealed a distinctive pattern of organ specified differential expression. Grafting experiments showed that the abnormal shape of tubers of transformed potato was probably determined by TL-DNA products synthesised within the tuber and not by diffusable products synthesised in other parts of the plant. The abundance of at least one transcript, tr5, was probably determined by culture conditions. Implications for functions and control of expression of Ri TL-DNA genes are discussed. It is suggested that Ri TL-DNA provides a convenient and extensive set of model genes to study variation and stability of expression of linked foreign genes introduced into plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00034939
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