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  • Rat  (11)
  • 36.40
  • Springer  (16)
  • American Chemical Society
  • American Institute of Physics
  • American Physical Society
  • 2010-2014
  • 1985-1989  (16)
  • 1986  (16)
Collection
Keywords
Publisher
  • Springer  (16)
  • American Chemical Society
  • American Institute of Physics
  • American Physical Society
Years
  • 2010-2014
  • 1985-1989  (16)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Quantitative changes in the lysosomal vacuolar system of rat hepatocytes during short-term starvation (1986)
    Springer
    Cell & tissue research 243 (1986), S. 641-648 
    Details
    ISSN: 1432-0878
    Keywords: Hepatocytes ; Lysosomes ; Macroautophagy ; Microautophagy ; Starvation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural morphometric analysis was used to study time-dependent variations in macro and microautophagy in rat hepatocytes. Except during periods of shortterm starvation for up to 24 h, animals were kept under standardized conditions of food intake. In hepatocytes of meal-fed rats the volume fraction of macroautophagic vacuoles is significantly higher at 23:00 h, i.e., immediately before food intake, compared to 11:00 h, i.e., 12 h following feeding. During fasting, macroautophagy drops to a low level. Microautophagic vacuoles in hepatocytes of meal-fed rats, sacrificed at 11:00 or 23:00 h respectively, do not show any significant quantitative differences. However, during 12 h of starvation, the volume fraction of microautophagic vacuoles rises significantly, whereas the numerical density remains constant. Subsequently, during the second 12-h period of fasting, the volume fraction of microautophagic vacuoles remains unchanged, but the numerical density increases. Over a period of 24 h of starvation the volume fraction of the total lysosomal system does not change significantly, whereas the numerical density rises. The time-dependent changes of the macroautophagic vacuolar system correlate with the circadian, food-related variations in the protein content of individual hepatocytes from meal-fed animals. The increase in volume fraction and thereafter in number of microautophagic vacuoles, as observed during starvation, coincides with a large decrease in protein content of individual hepatocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218073
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  • 2
    Electronic Resource
    Electronic Resource
    Experimental study of the dissociation of 100–600 keV hydrogen cluster ions in an argon gas target (1986)
    Springer
    The European physical journal 2 (1986), S. 87-90 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 34.90
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We have studied the break-up of accelerated hydrogen cluster ions passing through an argon gas target. The absolute dissociation cross section has been measured for a wide variety of H n + (odd masses only) cluster ions, withn between 5 and 23 and with projectile velocities ranging from 1.5 to 5 × 108 cm/s. We discuss the dissociation processes and the dependence of their cross-sections upon the cluster mass and velocity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01437246
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in the uropygial gland of the male Japanese quail, Coturnix coturnix, after testosterone treatment (1986)
    Springer
    Cell & tissue research 246 (1986), S. 373-378 
    Details
    ISSN: 1432-0878
    Keywords: Uropygial gland ; Sebaceous gland ; Testosterone ; Japanese quail ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the uropygial gland of the male quail was compared to that of the sebaceous gland of the male rat after castration and testosterone treatment of both species. In intact animals, the differentiating cells of these glands displayed almost the same pattern as regards their smooth endoplasmic reticulum, an organelle involved in lipogenesis in both cases. Castration reduced the volume of this organelle, while testosterone administration restored cell morphology to a normal or supranormal level. Finally, this study showed that at ultrastructural level, there is a close functional analogy between the uropygial gland of quail and the sebaceous glands of rats as regards their androgen dependency. Consequently, the uropygial gland might be an attractive model for study of action of androgens on sebaceous-like glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215900
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  • 4
    Electronic Resource
    Electronic Resource
    Light- and electron-microscopic visualization of γ-aminobutyric acid and GABA-transaminase in the oviduct of rats (1986)
    Springer
    Cell & tissue research 244 (1986), S. 621-626 
    Details
    ISSN: 1432-0878
    Keywords: GABA ; GABA-transaminase ; Oviduct ; Cilia ; Basal bodies ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution in the rat oviduct of γ-aminobutyric acid and its catabolic enzyme GABA-transaminase was studied by the use of immunocytochemical and enzymehistochemical techniques. At the light-microscopic level, both GABA immunoreactivity and GABA-transaminase enzyme reactivity were found primarily in the tubal epithelium while in the muscle layers of the organ only a faint GABA and GABA-transaminase positive staining could be detected. Electron-microscopic evaluation of the GABA immunoreactivity revealed a heavy labelling of the basal bodies (kinetosomes) and a moderate staining of the cilia. These findings indicate that the role of GABA in the oviduct is not related to neurotransmission but may be related to ciliary functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212542
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  • 5
    Electronic Resource
    Electronic Resource
    Naphthol-yellow-S protein content of individual rat hepatocytes as related to food intake and short-term starvation (1986)
    Springer
    Cell & tissue research 243 (1986), S. 165-169 
    Details
    ISSN: 1432-0878
    Keywords: Hepatocytes ; Liver ; Protein mass ; Starvation ; Proteolysis ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With regard to the protein content, as analysed cytophotometrically, of hepatocytes from rats kept under a 12L 12D photoperiod (photophase 7:00–19:00), the following facts have been established: 1) Hepatocytes of different classes of ploidy all demonstrate, more or less equally, daily variations in protein content and also its reduction after 24-h fasting. 2) With computer analysis of data obtained at eight time points during a period of 24 h, a sinusoidal curve of the protein content of individual mononuclear tetraploid hepatocytes throughout the day could be demonstrated with a maximum at 6:20 and a minimum at 18:20. 3) Animals, fed with meals via a dispensing machine from 23:00 to 24:00 only, show a similar sinusoidal curve but with higher amplitude, and a virtually identical mean value as those fed ad libitum. The maximum was found at 10:40, revealing a time lag of 12 h after food intake, the minimum at 22:40. 4) Trained animals deprived of food during the standardized feeding time revealed a moderate reduction of their hepatocyte protein content in the first 6 h, then a 6-h period with a steep fall followed by a slower reduction. After 24 h, the mean hepatocyte protein mass had decreased to 72% of that at the commencement of fasting at 23:00.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221865
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of ATP inhibitors on the translocation of luminal membrane between cytoplasm and cell surface of transitional epithelial cells during the expansion-contraction cycle of the rat urinary bladder (1986)
    Springer
    Cell & tissue research 246 (1986), S. 109-117 
    Details
    ISSN: 1432-0878
    Keywords: Urinary bladder ; Discoidal vesicles ; ATP ; Morphometrics ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Movement of asymmetric membrane plaques between the cytoplasm and surface of luminal urothelial cells was investigated during artificially induced contraction and expansion of untreated and ATP-depleted urinary bladders of the rat. Estimations of surface area, volume, and number of discoidal vesicles per unit volume of cytoplasm were determined by morphometric examination of electron micrographs. These values were compared in luminal cells from bladders incubated in control media or in media containing 0.15 mM 2,4-dinitrophenol and 0.02 mM sodium arsenate. The ATP inhibitors had no apparent effect upon the contraction of apical cells that had been incubated in an expanded state. In contrast, after distension of poisoned, contracted bladders, the orientation of intermediate filaments and the densities of discoidal vesicles were similar to the condition characterized by contracted cells. The results indicated that the normal reorientation of filaments, coincident with cell distension, had been suppressed by ATP inhibitors. This, in effect, impeded the filament-mediated translocation of membrane plaques to the surface. The reduction of surface area along the luminal border forced many cells to compensate by separating at their lateral margins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219006
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  • 7
    Electronic Resource
    Electronic Resource
    Are opioid peptides co-localized with vasopressin or oxytocin in the neural lobe of the rat? (1986)
    Springer
    Cell & tissue research 246 (1986), S. 177-182 
    Details
    ISSN: 1432-0878
    Keywords: Vasopressin ; Oxytocin ; Opioid peptides ; Neurosecretion ; Neural lobe ; Co-localization ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The content of vasopressin, oxytocin, neurophysin, leucine-enkephalin, methionine-enkephalin, dynorphin-(1–13), and α-neoendorphin in the rat neurohypophysis was measured after different periods of dehydration and after depolarisation of isolated neural lobes and of neurosecretory nerve endings. The rates at which the amount of neurohypophysial hormone and opioid peptides decreased, and the changes in the ratios between the amount of vasopressin or oxytocin and opioid peptide in the neurohypophysis after dehydration and in the incubation medium after depolarization in vitro cast some doubt on, and can be explained by mechanisms other than co-localisation of the different peptides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219015
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  • 8
    Electronic Resource
    Electronic Resource
    A possible phagocytic role for folliculo-stellate cells of anterior pituitary following estrogen withdrawal from primed male rats (1986)
    Springer
    Cell & tissue research 243 (1986), S. 255-261 
    Details
    ISSN: 1432-0878
    Keywords: Anterior pituitary gland ; Estrogen treatment ; Intercellular junctions ; Folliculo-stellate cells ; Phagocytosis ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural changes suggesting a phagocytic role for the nongranular folliculo-stellate cells of the anterior pituitary are investigated in estrogen-primed male rats after withdrawal of estrogen. Morphological changes in mammotropes following the removal of a subcutaneous estradiol-containing Silastic implant include the formation of intracellular lipid bodies. These lipid bodies appear to be associated with enhanced estrogen-dependent prolactin secretion in mammotropes. Seven and 24 h after estrogen withdrawal intracellular lipid within mammotropes seems to be released into the intercellular space. Seventy-two h after estrogen withdrawal, lipid droplets are almost entirely cleared from mammotropes while folliculo-stellate cells become packed with lipid globules. Folliculo-stellate cells also undergo dramatic hypertrophy 7 and 24 h after the removal of E2-containing implants. Extensive intercellular junctions including zonulae adhaerentes, desmosomes, and putative gap junctions are formed. Intercellular junctions delineate extravascular channels into which numerous microvilli project. Folliculo-stellate cells appear capable of accumulating many lipid droplets, presumably related to mammotrope metabolism. What appear to be large secondary lysosomes as well as the lipid droplets are observed within folliculostellate cells; lipid, therefore, may be degraded through a lysosomal pathway in folliculo-stellate cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251039
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  • 9
    Electronic Resource
    Electronic Resource
    Changes occurring in the epithelium covering the bronchus-associated lymphoid tissue of rats after intratracheal challenge with horseradish peroxidase (1986)
    Springer
    Cell & tissue research 245 (1986), S. 439-444 
    Details
    ISSN: 1432-0878
    Keywords: BALT ; Lymphoepithelium ; IgA response ; Ia-expression ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Changes occurring in the epithelium covering bronchus-associated lymphoid tissue (BALT) in the rat after several intratracheal administrations of horseradish peroxidase (HRP) were studied using morphological and ultrastructural methods. The epithelium is invaded by W3/ 25-positive (T-helper) lymphocytes, the BALT epithelial cells become Ia-positive and develop microvilli; there is an apparent loss of cilia. The number of non-ciliated cells in stimulated BALT increases. The non-ciliated cells can be subdivided into two cell types, one with electron-dense cytoplasm and cytoplasmic granules and the other without granules. The electron-density of the latter cell type is intermediate between that of the ciliated cells and that of the granulecontaining non-ciliated cells. The granule-containing cell types may be responsible for the uptake of antigens, while the other non-ciliated cell may be involved in the production of the secretory component and the passage of secretory IgA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213952
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  • 10
    Electronic Resource
    Electronic Resource
    Acute response of testicular interstitial tissue in rats to the cytotoxic drug ethane dimethanesulphonate (1986)
    Springer
    Cell & tissue research 243 (1986), S. 405-414 
    Details
    ISSN: 1432-0878
    Keywords: Leydig cells ; Ethane dimethanesulphonate ; Ultrastructure ; Destruction ; Gonadotrophins ; Testosterone ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cytotoxic effects of ethane dimethanesulphonate upon rat Leydig cells were examined ultrastructurally up to 3 days after treatment and related to changes in serum levels of gonadotrophins and testosterone. Six hours after administration of ethane dimethanesulphonate the usual tubulo-vesicular morphology of Leydig-cell smooth endoplasmic reticulum was converted to small vesicles and the Golgi apparatus showed focal hypertrophy into anastomosing tubules. These changes became more marked by 12 h with many Leydig cells exhibiting karyopyknosis and hyperchromatism. Necrotic Leydig cells were often engulfed by macrophages, the latter containing pyknotic fragments of Leydig cells within their cytoplasm. One day after administration, advanced necrosis of Leydig cells occurred, many of which were phagocytosed by macrophages, and on day 3, destruction of Leydig cells was complete resulting in their elimination from the interstitial tissue, which contained only loose connective tissue and macrophages. Structural alterations to the Leydig cells from 6–24 h was reflected by a significant reduction in serum testosterone levels which further declined to the limits of detection accompanying the abolition of Leydig cells on day 3. These changes were paralleled by a significant elevation of serum LH and FSH levels suggesting diminished feedback regulation of pituitary gonadotrophin secretion. The results indicate that ethane dimethanesulphonate is a rapidly acting Leydig cell toxin which may be a useful experimental tool in further studies of spermatogenic function mediated via Sertoli cell-Leydig cell interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251057
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