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  • Articles  (71)
  • Life and Medical Sciences  (71)
  • Wiley-Blackwell  (71)
  • American Chemical Society
  • American Physical Society
  • Cambridge University Press
  • International Union of Crystallography (IUCr)
  • Springer
  • 1985-1989  (71)
  • 1980-1984
  • 1965-1969
  • 1955-1959
  • 1945-1949
  • 1985  (71)
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  • Articles  (71)
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  • Wiley-Blackwell  (71)
  • American Chemical Society
  • American Physical Society
  • Cambridge University Press
  • International Union of Crystallography (IUCr)
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  • 1985-1989  (71)
  • 1980-1984
  • 1965-1969
  • 1955-1959
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  • 1
    Electronic Resource
    Electronic Resource
    Incorporation of [3H]thymidine by isolated fetal myoblasts and fibroblasts in response to human placental lactogen (HPL): Possible mediation of HPL action by release of immunoreactive SM-C (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 125 (1985), S. 337-344 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We investigated the actions of human placental lactogen (HPL) and human growth hormone (HGH) on [3H]thymidine incorporation and the release of immunoassyable somatomedin-C (SM-C) by isolated myoblasts, dermal fibroblasts, and costal cartilage explants taken from human fetuses, at 11-21 weeks of gestation. The incorporation of [3H] thymidine by myoblasts and fibroblasts was significantly increased after incubation for 20 hr or 44 hr, and cell number after incubation for 7 days, in the presence of 50-250 ng/ml HPL. Incubation with HPL did not increase [3H]thymidine incorporation into cartilage explants, whereas incubation with HGH failed to enhance the uptake of this isotope by any of the tissues. Following extraction with acid-ethanol, culture medium conditioned by exposure to myoblasts or fibroblasts for 44 hr, and to cartilage explants for 7 days, contained radioimmunoassayable SMC. Myoblast-conditioned medium contained significantly more SM-C [1,609 ± 953 mU/mg cell protein (mean ± SD) n = 10] than did that conditioned by fibroblasts (637 ± 323; n = 5; P 〈 0.02). In 1 week of culture, cartilage explants released 4.1 ± 1.1 mU/mg wet weight (n = 7). The release of immunoassayable SM-C from cultured cells was significantly increased in the presence of 250 ng/ml HPL in five of eight experiments with myoblasts and two of four experiments with fibroblasts. Neither fibroblasts or myoblasts showed increased SM-C release following exposure to HGH.The results suggest that HPL, but not HGH, is growth-promoting for some human fetal tissues in vitro and that this action is mediated, at least in part, by an increased release of somatomedins.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041250224
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  • 2
    Electronic Resource
    Electronic Resource
    Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1 (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 125 (1985), S. 403-412 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We recently derived a series of transformed cell lines by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with a newly described human gene, R-myc, which has homology to the c-myc oncogene. In this report, we show that these lines share some features characteristic of cells of the mononuclear phagocyte lineage. Specifically, all cell lines had macrophage-or monocytelike morphology, contained nonspecific esterase, were phagocytic for latex beads, secreted lysozyme, bore the Mac-1 antigen, and contained a minority of cells with Fc receptors. However, only a single monocytelike clone had appreciable numbers of cells which bore complement receptor 1, and none were phagocytic for antibody or complementcoated particles, or constitutively secreted Interleukin-1. All these cell lines secreted a growth factor capable of supporting the in vitro proliferation of bone marrow macrophages. Radiommunoassay and receptor binding studies indicate that this factor is colony stimulating factor 1.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041250307
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro and in vivo studies of mammalian sperm nuclear decondensation (1985)
    New York, NY : Wiley-Blackwell
    Gamete Research 11 (1985), S. 349-365 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; mammalian ; fertilization ; mammalian ; nucleus ; spermatozoon ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120110403
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  • 4
    Electronic Resource
    Electronic Resource
    What the papers say: Role of hepatic glycolysis and gluconeogenesis in glycogen synthesis (1985)
    New York, NY : Wiley-Blackwell
    BioEssays 2 (1985), S. 273-276 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950020609
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  • 5
    Electronic Resource
    Electronic Resource
    The ultrastructure of the cardiac ganglion of the desert scorpion, Paruroctonus mesaensis (Scorpionida: Vaejovidae) (1985)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 184 (1985), S. 231-252 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Light and electron microscopy of the pacemaker ganglion of the scorpion heart indicate that it is about 15 mm long and 50 μm in diameter and extends along the dorsal midline of the heart. The largest cell bodies (30-45 μm in diameter) occur in clusters along the length of the ganglion. The ganglion appears to be innervated with fibers from the subesophageal and first three abdominal ganglia.The cardiac ganglion is surrounded by a neurilemma and a membranous sheath. The latter is apparently derived from connective tissue cells seen outside the ganglion. Nerve fibers other than those in the neuropil areas are usually surrounded by membrane and cytoplasm of glial cells. Often there are several layers of glial membrane, forming a loose myelin. The cardiac nerves to the heart muscle are also surrounded by a neurilemma, and the axons are surrounded by glia. The motor nerves contain lucent vesicles 60-100 nm and opaque granules 120-180 nm in diameter.In the cardiac ganglion, some nerve cell bodies have complex invaginations of glial processes forming a peripheral trophospongium. In the neuropil areas, nerve cell processes are often in close apposition. The septilaminar configuration typical of gap junctions is common, with gap distances of 1-4 nm. In tissues stained with lanthanum phosphate during fixation, we found gaps with unstained connections (1-2 nm diameter) between nerve-nerve and glial-nerve cell processes. Annular or double-membrane vesicles in various stages of formation were also seen in some nerve fibers in ganglia stained with lanthanum phosphate.Nerve endings with electron-lucent vesicles 40-60 nm in diameter are abundant in the cardiac ganglion, suggesting that these contain the excitatory transmitter of intrinsic neurons of the ganglion. Less abundant are fibers with membrane-limited opaque granules, circular or oblong in shape and as much as 330 nm in their longest dimension. Also seen were some nerve endings with both vesicles and granules.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051840212
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  • 6
    Electronic Resource
    Electronic Resource
    The intrarenal and pericapsular venous systems of kidneys of the ringed seal, Phoca hispida (1985)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 184 (1985), S. 361-373 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The kidneys of Phoca hispida are comprised of many closely adherent renculi, each of which is a small kidney, functionally independent of its neighbours except with respect to venous drainage. Venous blood from the rencular parenchyma drains to the periphery through interlobular veins. These interlobular veins empty into a perirencular plexus comprised of subcapsular veins on the free surface of the renculus, interrencular veins on adjoined surfaces, and marginal subcapsular veins lying in the furrows between adjoined renculi. A pericapsular plexus of large veins overlies the marginal subcapsular veins and has frequent connections with them. Blood drains from the pericapsular plexus into large superficial collecting veins that converge over the surface of the kidney toward the divided hilum and connect directly to the paired trunks of the posterior vena cava. There are also connections to other major venous systems of the region.There is no arcuate venous system, no major vein at the rencular hilum, and no vein of consequence emerging from the renal hilum. Venous outflow is virtually entirely directed to the peripheral plexuses. The venous pattern differs from that of most mammals in which blood drains from the renal parenchyma to arcuate veins and leaves the kidney through a renal vein, or veins, emerging from the hilum.The walls of veins in the kidney are remarkably thin in comparison to their size. Subcapsular veins up to 0.5 mm wide have walls on the parenchymal side that in places consist only of a thin, fenestrated endothelium and a basal lamina.
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051840310
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  • 7
    Electronic Resource
    Electronic Resource
    Functional characteristics of the calf muscles of the rat (1985)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 184 (1985), S. 375-387 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Length-force relations, both active and passive, and twitch contraction characteristics were quantified for the entire complex of the superficial calf muscles, as well as individually for the Mm. soleus, plantaris, and gastrocnemius, caput mediale and laterale, of eight male Wistar rats. The M. soleus composes approximately 5% of the weight and cross-sectional area of the entire group of superficial calf muscles and is the only muscle of the group containing mainly slow-twitch fibers. The other superficial muscles of the calf are primarily fast-twitch muscles.The mono-articular M. soleus, the bi-articular M. gastrocnemius, caput mediale and laterale, and the poly-articular M. plantaris differ with respect to the number of joints crossed. However, contrary to the findings for cat hind limbs (Goslow et al. [1977] J. Morphol. 153:23-38), the muscles of the complex of superficial calf muscles of the rat did not differ with respect to (a) their fiber optimum length, (b) their maximum length range of active force generation, (c) the relative increase of passive force owing to lengthening of the muscle, (d) the angle of the ankle at which they produce maximal active force (the knee angle was fixed at 90°).
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051840311
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  • 8
    Electronic Resource
    Electronic Resource
    Tooth replacement and growth in the young green iguana, Iguana iguana (1985)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 186 (1985), S. 265-269 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A study using eight rapidly growing young green iguanas (Iguana iguana; initial mean weight 68.0 ± 3.8 gm) examined the changes in the wave replacement of teeth, the increased size of the teeth, and the posterior migration of tooth positions over a period of 16 weeks. The teeth increase in width as the lizards grow. The tooth positions shifted posteriorly, providing adequate space for the larger replacement teeth. These observations suggest that the wave replacement of teeth allows for growth of the dentition in length and height adequate to maintain tooth size in proportion to the overall size of the individual.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051860303
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  • 9
    Electronic Resource
    Electronic Resource
    Relationship of symbiotic microorganisms to metanephridium: Phagoctic activity in the metanephridial epithelium of two species of oligochaeta (1985)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 186 (1985), S. 307-314 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: It is known that a number of species in the annelid family Lumbricidae harbor symbiotic microorganisms in the lumen of their nephridia. The purpose of the present paper is the study of the relationship between microbes and epithelial cells lining the metanephridium of two species of Oligochaeta, which show two different patterns of microbial colonization. A new interesting feature, the phagocytosis and intracellular destruction of microorganisms by the nephridial epithelial cells, has been observed in our laboratory for the first time. In Scheroteca savignyi minor, the phagocytic activity takes place in the bladder, the most distal region of the nephridium, next to the nephridiopore, which may prevent the microorganisms from entering the more proximal regions. In Octolasion cyaneum the microbes reach the striated duct, where they live in symbiosis, adhere to the cell's surface, and are engulfed and destroyed by the cells of the middle tubule - the more proximal, neighboring region. The phagocytosis and intracellular degradation of microorganisms probably lead to the massive formation of lamellar bodies, which are observed in these cells and in the neighboring ones.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051860307
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  • 10
    Electronic Resource
    Electronic Resource
    Flow birefringence of microtubules and its relation to birefringence measurements in cells (1985)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 5 (1985), S. 31-51 
    Details
    ISSN: 0886-1544
    Keywords: microtubules ; birefringence ; flow birefringence ; tubulin ; polarization microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Understanding the molecular basis of mitotic movements in living cells will require correlative experiments on intact cells, cell models, purified tubulin, and perhaps other biopolymers. Birefringence is one assay that is useful in all of these experimental situations. Heretofore, studies of birefringence changes during mitosis have lacked a quantitiative basis for interpretation in terms of microtubule number and packing density. One of the aims of this work was to establish that relationship.Purified calf brain tubulin was polymerized to equilibrium and oriented in the hydrodynamic field of a microcapillary flow birefringence apparatus. The relationship between birefringence and microtubule packing density was determined by a combination of optical, electron microscopic, and biochemical methods. The data correlate surprisingly well with those obtained by others from in vitro measurements on isolated mitotic spindles. Using the flow birefringence data, the sensitivity of polarizing microscopes for detecting microtubules was examined and found to depend on microtubule packing density, object thickness, and instrumental factors that limit both the detection and measurement of weakly birefringent objects. Because of the dependence of measurement sensitivity on object thickness, a method of measuring the thickness of microtubule bundles using the dispersion of birefringence was developed. This method is capable of measuring thickness to within two or three Airy diffraction units and does not require any assumptions regarding object symmetry.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970050104
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