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  • ASTRONOMY  (72)
  • Biochemistry and Biotechnology
  • 1985-1989  (116)
  • 1985  (116)
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 1572-1576 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2011-08-19
    Description: VLBI observations at 2.29 GHz with fringe spacings of about 3 milliarcsec have been performed on 1398 radio sources spread over the entire sky. 917 sources were detected, including 93 percent of the identified BL Lacertae objects, 86 percent of the quasars, and 36 percent of the galaxies. The resulting catalog of compact radio sources is useful for various astrophysical studies and in the formation of VLBI celestial reference frames.
    Keywords: ASTRONOMY
    Type: Astronomical Journal (ISSN 0004-6256); 90; 1599-164
    Format: text
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  • 3
    Publication Date: 2011-08-19
    Description: VLBI measurements at 2290 and 8420 MHz on baselines of 10,000 km have been used to determine the positions of the milliarcsecond nuclei in 74 extragalactic radio sources. Estimated accuracies range from 0.1 to 4.3 arcsec in both right ascension and declination with typical accuracies of about 0.3 arcsec. The observed sources are part of an all-sky VLBI catalog of milliarcsecond radio sources. Arcsecond positions have been determined for 819 sources. These positions are presently being used to identify optical counterparts in the southern hemisphere.
    Keywords: ASTRONOMY
    Type: Astronomical Journal (ISSN 0004-6256); 90; 590-594
    Format: text
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 603-612 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: We describe the use of affinity chromatography for separation of cell populations that do not differ significantly with respect to gross physical properties such as size, density, or charge. Cell affinity chromatography exploits differences in cell surface macromolecules by passage of mixtures of cell populations through a column containing beads to which are attached chemical ligands with specific binding affinity for particular cell surface receptors. In this article we focus on the application of this concept to separation of mature T lymphocytes from peripheral blood. This serves as a model for the separation of these cells from bone marrow in order to prevent graft-vs.-host disease in bone marrow transplantation. However, the concept of cell affinity chromatography should find more general widespread utility in a variety of biotechnological applications. Thus, we introduce a simple theoretical framework which is necessary in order to understand the results that might be expected in any given situation. Finally, we use this theory to provide a tentative explanation for experimental observation of the effects of temperature and flowrate on the degree of separation achieved for our current pplication.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 842-851 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Immobilization experiments have been performed with glucose oxidase as enzyme and controlled-pore glass of different pore sizes as support for chemical coupling. The experimental results have been analyzed for comparison with the theoretical model predictions. Analysis of the initial stage of the process gives the fundamental characteristic of the immobilization reaction. These investigations allow us to study the influence of the degree of diffusional restriction on the evolution of the immobilization process and spatial distribution of immobilized enzyme. Nonuniformly distributed concentrations have been achieved within the porous matrix, and suggestions have been made in designing such profiles by choosing appropriate experimental parameters.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 1126-1135 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article deals with the mathematical modeling of the process of enzyme immobilization in porous membranes. During the initial period, an analytical solution is available to extract the rate constant for immobilization. Beyond this period, the model equations are solved numerically to yield the transient response of the enzyme concentration in the immobilizing solution and also the evolution of the enzyme loading profile inside the membrane. It is found that the immobilization practically ceases even through the attachment sites are still available within the membrane.
    Additional Material: 8 Ill.
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  • 7
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The growth of the hydrocarbon-rich alga Botryococcus braunii was studied under “air-lift” conditions using batch and continuous cultures. Large variations in the physiological state of B. braunii were achieved in batch cultures and in continuous cultures with various dilution rates. The possible effects of these variations upon hydrocarbons (nature, relative abundance, location, level, productivity) and also on the production of exocellular polysaccharides were examined. The relationships between the physiological state of B. braunii and its hydrocarbon and polysaccharide production were discussed and compared with those generally observed in unicellular algae. The factors giving rise to the transition from high to low productivity stages were considered. To this end we examined, at first, the variations in cell ultrastructure and the resulting degeneration occurring during batch cultures. Afterward the parallel changes in some parameters of the medium (pH, phosphate level) were determined and their possible relationships with B. braunii growth and hydrocarbon production were discussed. The main features of phosphate nutrition in B. braunii and its effects on hydrocarbons were finally examined.
    Additional Material: 7 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 883-886 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
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  • 9
    Publication Date: 2011-08-19
    Description: Differential VLBI was simultaneously performed on the source pair GC 1342 + 662 and GC 1342 + 663 (4.4-arcminute separation) using S-band on the Goldstone/Madrid baseline. These measurements were acquired on two separate observing sessions: 30 December 1982 and 14 May 1983. The change in separation of GC 1342 + 662 relative to GC 1342 + 663 between the two epochs was 0.03 + or - 0.08 milliarcsecond. The differences of the relative position measurements between epochs of GC 1342 + 662 relative to GC 1342 + 663 were -0.29 + or - 0.05 milliarcsecond in right ascension and 0.14 + or 0.09 milliarcsecond in declination. These measurements demonstrate submilliarcsecond accuracy and repeatability. The discrepancies outside of the formal uncertainties could be attributed to the intrinsic properties of the sources such as structure and to a lesser probability, proper motion. These discrepancies could also be attributed to excursions in UT1-UTC of about four times the quoted BIH uncertainty.
    Keywords: ASTRONOMY
    Type: Astronomical Journal (ISSN 0004-6256); 90; 1004-100
    Format: text
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  • 10
    Publication Date: 2011-08-19
    Description: Radio- and visible-wavelength observations for the BL Lac object 1219 + 28 (ON 231, W Com) are reported along with optical observations of 0851 + 202 (OJ 287), and radio data for 1400 + 162 (4C 16.39, OQ 100). The obtained data consist of imaging and surface photometry for both 1219 + 28 and 0851 + 202, as well as spectra for 1219 + 28, and Very Long Baseline Interferometry (VLBI) observations for 1219 + 28 and 1400 + 162. Two emission features have been observed in the spectrum of 1219 + 28. Radio observations of 1219 + 28 in three frequencies reveal the presence of a radio jet with at least three components. The obtained results for 1219 + 28 support previous conclusions that at least some BL Lac objects are located at the center of elliptical galaxies.
    Keywords: ASTRONOMY
    Type: Astrophysical Journal, Part 1 (ISSN 0004-637X); 292; 614-619
    Format: text
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