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  • Blackwell Publishing Ltd  (14)
  • Canadian Science Publishing
  • 2000-2004  (5)
  • 1985-1989  (12)
  • 2003  (5)
  • 1985  (12)
  • 1
    Electronic Resource
    Electronic Resource
    Trypanosoma brucei: Differentiation of in Vitro-Grown Bloodstream Trypomastigotes into Procyclic Forms (1985)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 32 (1985), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Trypanosoma brucei strain 366D trypomastigotes grown at 37°C in the presence of a human fibroblast cell line formed foci underneath the feeder cells whereas trypanosomes grown in the presence of a human epithelial cell line grew only in the culture supernatant. A culture system was developed to study the differentiation of bloodstream trypomastigotes grown in the epithelial cell system into procyclic trypomastigotes at 27°C. The morphological differentiation into the procyclic form was complete by 48 h. Cell division did not occur until 30–40 h after transfer to 27°C. Various characteristics of this system were examined, including the effect of the feeder layer, the type of medium, the presence of the metabolites cis-aconitate and citrate, the preadaptation period, and the trypanosome cell concentration. The respiration of the recently differentiated procyclic cells was less sensitive to inhibition by CN-than that of established procyclic forms, implying a delayed appearance of complete mitochondrial oxidative pathways. This trypanosome differentiation system has the advantage that the animal host is not needed and the entire process is carried out in in vitro culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1985.tb03100.x
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  • 2
    Electronic Resource
    Electronic Resource
    The communicative power of expert systems (1985)
    Oxford, UK : Blackwell Publishing Ltd
    R & D management 15 (1985), S. 0 
    Details
    ISSN: 1467-9310
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: ‘Expert Systems’ or intelligent knowledge-based computer systems can be expected to have a considerable impact in the near future on the dissemination and use of technical expertise in practical situations. The potential benefits of such an approach to decision-making in applied science domains, for example, in anti-corrosive coating selection, are considerable. This paper argues, therefore, that a premium must be placed on such a system's performance as a communicator.The structure and behaviour of a classical ‘production system’ are briefly described, and the communicative ability of such a system is analysed. This critical examination of a production system's question-answering capabilities highlights the inherent restrictions which the structure of this kind of system places on its explanatory and justificatory powers. An outline design for a less inhibitive structure is presented.It is argued that the form in which the system provides ‘answers’ after the completion of a problem-solving session, may not yield sufficient appropriate information to the user, and a possible alternative view of a ‘solution schema’ is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1467-9310.1985.tb00528.x
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  • 3
    Electronic Resource
    Electronic Resource
    Development of RAPD markers associated with common bunt resistance to race T1 (Tilletia tritici) in spelt wheat (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Common bunt caused by Tilletia tritici and T. laevis has occurred worldwide and reduces yield and quality in common and durum wheats. The development of DNA markers linked to bunt resistance to race T1 in the cross, ‘Laura’(S) בRL5407’ (R), was carried out in this study based on the single head derived F4:5 and single seed derived F4:6 populations. Bulked segregant analysis was used to identify two random amplified polymorphic DNA (RAPD) markers linked to the gene for resistance to race T1 in the spelt wheat ‘RL5407′. The two markers identified, UBC548590 and UBC274988, flanked the resistance gene with a map distance of 9.1 and 18.2 cM, respectively. The former was linked in repulsion phase to bunt resistance while the later was in coupling phase. The two RAPD markers and the common bunt-resistance gene all segregated in Mendelian fashion. Use of these two RAPD markers together could assist in incorporating the bunt-resistance gene from spelt wheat into common wheat cultivars by means of marker-assisted selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00888.x
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  • 4
    Electronic Resource
    Electronic Resource
    Transmission properties of an oak canopy in relation to photoperception (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 8 (1985), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The spectra of incoming daylight and shadelight in a mature oak woodland were measured at intervals during the canopy cycle, and mean transmittance spectra were derived. Transmittance was spectrally neutral at ca. 0.55 during the light phase but, following leaf emergence, transmittance of PAR (400–700 nm) fell to ca. 0.1. Simultaneously, the red : far-red transmittance ratio fell to circa 0.6. Both showed little change during the summer and autumn until senescence, indicating that the optical properties of the canopy were surprisingly stable. There was no evidence that cloud cover influenced mean canopy transmittance, although transient sunflecks introduced great variability which, in combination with sampling bias, might explain previous contradictory reports. The red : far-red fluence rate ratio in the woodland showed a temporary increase in late summer, a result of a small increase in the red : far-red ratio of incoming daylight during this period. Reflectance and transmittance spectra and pigment content of sun and shade leaves were measured. Leaf transmittance spectra showed changes correlated with those of the canopy, and were related to changes in pigment content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1985.tb01686.x
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  • 5
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of early infection in the uredial stage of Puccinia sorghi in Zea mays (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 34 (1985), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An SEM study was made of the infection process of Puccinia sorghi in Zea mays. A uredospore germ tube grows across epidermal cells and along their anticlinal walls, often branching and altering direction of growth. The fungus, on attaining a stoma, delimits an appressorium over it. Infection peg initials enlarge linearly and centripetally along the appressorium base, forcing open the stomatal slit. Having penetrated the stomatal aperture, the infection peg develops a substomatal vesicle. From the vesicle, two short primary infection hyphae develop synchronously, a septum later forming between the vesicle body and each hyphal base. A further septum divides the primary hypha into two cells. Secondary infection hyphae emerge later from the fully expanded vesicle on the proximal side of each vesicle/primary hypha septum. Secondary hyphae are narrower than primary hyphae, form their proximal septum some distance along the hypha, develop asynchronously, and proliferate to form the intercellular mycelium. Infection processes and epidermal stripping are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1985.tb02761.x
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  • 6
    Electronic Resource
    Electronic Resource
    Liquid Chromatographic Analysis of Sugars and Mannitol in Cabbage and Fermenting Sauerkraut (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 50 (1985), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: High-performance liquid chromatography was used to measure concentrations of fructose, glucose and sucrose in several cultivars of cabbage as well as residual sugars and mannitol in sauerkraut. Cabbage cultivars contained from about 3.0-6.0% total sugars, and glucose was the most abundant sugar in entire cabbage heads. All sugars in a selected commercial tank of fermenting sauerkraut were depleted after 4 wk. However, total sugar concentrations to 0.8% were found in a variety of commercial sauerkraut samples. Mannitol accumulated early in fermentations, but concentrations in sauerkraut decreased during latter stages of holding (through 18 wk) before processing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1985.tb10560.x
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  • 7
    Electronic Resource
    Electronic Resource
    Monitoring Treatment in Congenital Adrenal Hyperplasia Use of Serial Measurements of 17–OH–Progesterone in Plasma, Capillary Blood, and Saliva (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 458 (1985), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1985.tb14604.x
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  • 8
    Electronic Resource
    Electronic Resource
    Properties of the periplasmic nitrate reductases from Paracoccus pantotrophus and Escherichia coli after growth in tungsten-supplemented media (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 220 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Paracoccus pantotrophus grown anaerobically under denitrifying conditions expressed similar levels of the periplasmic nitrate reductase (NAP) when cultured in molybdate- or tungstate-containing media. A native PAGE gel stained for nitrate reductase activity revealed that only NapA from molybdate-grown cells displayed readily detectable nitrate reductase activity. Further kinetic analysis showed that the periplasmic fraction from cells grown on molybdate (3 μM) reduced nitrate at a rate of Vmax=3.41±0.16 μmol [NO3−] min−1 mg−1 with an affinity for nitrate of Km=0.24±0.05 mM and was heat-stable up to 50°C. In contrast, the periplasmic fraction obtained from cells cultured in media supplemented with tungstate (100 μM) reduced nitrate at a much slower rate, with much lower affinity (Vmax=0.05±0.002 μmol [NO3−] min−1 mg−1 and Km=3.91±0.45 mM) and was labile during prolonged incubation at 〉20°C. Nitrate-dependent growth of Escherichia coli strains expressing only nitrate reductase A was inhibited by sub-mM concentrations of tungstate in the medium. In contrast, a strain expressing only NAP was only partially inhibited by 10 mM tungstate. However, none of the above experimental approaches revealed evidence that tungsten could replace molybdenum at the active site of E. coli NapA. The combined data show that tungsten can function at the active site of some, but not all, molybdoenzymes from mesophilic bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(03)00122-8
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  • 9
    Electronic Resource
    Electronic Resource
    The FliS chaperone selectively binds the disordered flagellin C-terminal D0 domain central to polymerisation (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 219 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Assembly of each Salmonella typhimurium flagellum filament requires export and polymerisation of ca. 30 000 flagellin (FliC) subunits. This is facilitated by the cytosolic chaperone FliS, which binds to the 494 residue FliC and inhibits its polymerisation. Yeast two-hybrid assays, co-purification and affinity blotting showed that FliS binds specifically to the C-terminal 40 amino acid component of the disordered D0 domain central to polymerisation. Without FliS binding, the C-terminus is degraded. Our data provide further support for the view that FliS is a domain-specific bodyguard preventing premature monomer interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(02)01208-9
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  • 10
    Electronic Resource
    Electronic Resource
    Novel protein vaccine candidates against Group B streptococcal infection identified using alkaline phosphatase fusions (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 222 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Using an alkaline phosphatase-based genetic screening method, we identified a number of proteins that are potentially located on the outer surface of Group B streptococcus (Streptococcus agalactiae). In an enzyme-linked immunosorbent assay, antisera raised against two of the proteins, the streptococcal yutD homologue and a subunit of an ABC transporter, recognised clinically important serotypes of Group B streptococcus. In a neonatal rat model, purified IgG from the sera conferred significant levels of protection against a lethal challenge infection. The proteins identified show potential as protein subunit candidates for vaccines against Group B streptococcal disease in neonates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(03)00310-0
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