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  • Chemical Engineering  (75)
  • Life and Medical Sciences  (59)
  • 1980-1984  (134)
  • 1965-1969
  • 1984  (134)
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Verlag/Herausgeber
Erscheinungszeitraum
  • 1980-1984  (134)
  • 1965-1969
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 339-351 
    Details
    ISSN: 0148-7280
    Schlagwort(e): spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mrd.1120100402
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Intercellular bridges in ovaries of the newborn gerbil (1984)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 29-35 
    Details
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: This study describes intercellular bridges in the ovaries of neonatal gerbils. Electron microscopy has revealed the presence of true intercellular bridges, connecting oogonia or oocytes, in ovaries of newborn gerbils. The cytoplasm of the intercellular channels is similar to that of the connected cells, with mitochondria, smooth and rough endoplasmic reticulum, and free ribosomes present. Lysosomes are also occasionally present in the intercellular bridges and they may be involved in early waves of oocyte atresia. An electrondense substance, 350-500 Å thick, is located immediately beneath the unit membrane of the intercellular bridges. Accumulation of electron-dense material increases the thickness of the walls of the intercellular bridges, supporting and maintaining the patency of the channels. It is suggested that the intercellular channels probably allow the interchange of nutrients, organelles, and possibly regulatory materials as well.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jmor.1051800105
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  • 3
    Digitale Medien
    Digitale Medien
    A comparison of spindle concentrations in large and small muscles acting in parallel combinations (1984)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 243-252 
    Details
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: A small short muscle frequently acts across a joint in parallel with a vastly larger and longer muscle; therefore it should play a minimal role in the mechanical control of that joint. This study provides evidence suggesting that the small member of such a “parallel muscle combination” (PMC) may serve an important sensory feedback role. The spindle densities of large and small members of PMCs in man and the dog were determined and compared. Epaxial PMCs controlling canine intervertebral joints were dissected and tissue samples were embedded in paraffin, sectioned transversely to the muscles' long axis and, stained with hematoxylin-periodic acid-Schiff (PAS). Representative tissue sections were projected on to stereological grids and the percentage volume of spindles was determined. Data existing in the literature were used to ascertain spindle densities in human PMCs controlling joints in the cervico-occipital region and the extremities. The spindle density for each muscle in a group of PMCs controlling a particular motion was listed, and the mean spindle densities were determined for both the large and the small members of the group. Student's unpaired t test was used to determine the significance of the differences between mean spindle densities. Linear regression was calculated and the data were plotted graphically.In all PMCs examined, the spindle density of the small muscles was significantly higher than that of their large counterparts. It is therefore proposed that the small muscles of PMCs may function as “kinesiological monitors” generating important proprioceptive feedback to the central nervous system.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jmor.1051800307
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  • 4
    Digitale Medien
    Digitale Medien
    Thermal regeneration of adsorption beds: Equilibrium theory for solute condensation (1984)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 30 (1984), S. 679-682 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690300429
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  • 5
    Digitale Medien
    Digitale Medien
    Comments on the paper, “gas transport through polyethylene membranes” (1984)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 30 (1984), S. 492-494 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690300323
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  • 6
    Digitale Medien
    Digitale Medien
    Evolution of pore volume distribution during gasification (1984)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 30 (1984), S. 967-973 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A general equation is derived that predicts the evolution of pore volume distribution during isothermal gasification in the regime of kinetic control, starting from a given initial condition. The development takes into account pore enlargement as well as pore intersections. Equations are also derived for the special cases of (1) uniform pore size and (2) bimodal distribution. The results are used to interpret the experimental data of Tomkow et al. (1977) and Kawahata and Walker (1962).
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690300612
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  • 7
    Digitale Medien
    Digitale Medien
    Rate processes in cycling a reversible gas-solid reaction (1984)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 30 (1984), S. 56-62 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Rate studies are reported of the effect of rehydration-dehydration cycling on the vapor hydration behavior of solid K2CO3. Isothermal rate data were obtained at different temperatures and water vapor pressures for the reaction of narrowlysized anhydrous particles. Effects of different particle preparation histories on the rehydration rate were investigated and correlations of rate with particle pore structure explored. Rehydration rates of dehydrated K2CO3·3/2H2O were found to depend on the conditions of the prior dehydration. Rehydration is comparatively very slow at relative pressures below P/Peq ≃ 1.5; rates increase linearly with pressure above P/Peq ≃ 3. Hydration rates of K2CO3 particles obtained as anhydrous are substantially slower than those of identically-sized crystals produced by prior dehydration of K2CO3·3/2H2O; after one rehydration-dehydration cycle, rehydration rates are increased by as much as two orders of magnitude and this distinction between sources virtually disappears. Diffusional resistances based on calculated water vapor diffusivities are qualitatively consistent with the observed effects of cycling but do not by themselves account fully for the observations.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690300110
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  • 8
    Digitale Medien
    Digitale Medien
    Kinetics of hydration-dehydration reactions considered as solid transformations (1984)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 30 (1984), S. 557-563 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A mechanism is proposed for the dehydration-rehydration process in solid inorganic salts, and model rate equations are derived and applied to the observed behavior of potassium carbonate. Quantitative expressions for the effect of pressure on the reaction rates are derived using basic principles from nucleation and heterogeneous phase transformation theory. Model equation predictions agree with experimental dehydration and rehydration rate data at all but extreme pressures. The basic rate equation is also used to interpret the data of Eckhardt and Flanagan (1964) for the effect of pressure on the dehydration of manganous formate dihydrate. The mechanism on which the model equations are based is also consistent with the observed effects of cycling and of high temperature pretreatment on the K2CO3 rehydration rate.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690300405
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  • 9
    Digitale Medien
    Digitale Medien
    Catalytic conversion of large molecules: Effect of pore size and concentration (1984)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 30 (1984), S. 849-853 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690300531
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  • 10
    Digitale Medien
    Digitale Medien
    Simulation of pore growth and coalescence (1984)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 30 (1984), S. 829-831 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690300523
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