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  • Golgi apparatus  (7)
  • Ultrastructure  (5)
  • Springer  (12)
  • 1980-1984  (12)
  • 1983  (12)
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Keywords
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  • Springer  (12)
Years
  • 1980-1984  (12)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Stomatal pore and cuticle formation inFunaria (1983)
    Springer
    Protoplasma 116 (1983), S. 1-13 
    Details
    ISSN: 1615-6102
    Keywords: Cuticle ; Peristomatal transpiration ; Stomata ; Ultrastructure ; Funaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cuticle and pore development in the guard cells ofFunaria were investigated with the electron microscope. Pore cuticle formation is simultaneous with the creation of the pore itself. The morphology of the pore cuticle is unlike that of any cuticle described in the literature. It has many lamellae which are penetrated by electron dense fibrils. Three different cuticular morphologies exist from the pore to the subsidiary cell walls. The cuticles on the pore and outer walls contain fibrils that sometimes reach to the surface. The subsidiary cell cuticle lacks fibrils altogether. It is hypothesized that (1) cuticularization of the middle lamella contributes to ventral wall separation and (2) differences in extent of cuticular fibrils are related to greater water loss from stomata than from subsidiary cells (peristomatal transpiration).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01294225
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  • 2
    Electronic Resource
    Electronic Resource
    Topography, ultrastructure and phagocytic capacity of avian lymph nodes (1983)
    Springer
    Cell & tissue research 228 (1983), S. 389-403 
    Details
    ISSN: 1432-0878
    Keywords: Lymph node, avian ; Ultrastructure ; Macrophages ; Phagocytic capacity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of the avian lymph node (ALN) is characterized by a thin capsule, thin lymphoreticular cords, and an absence of trabeculae. It is not possible to subdivide the ALN into cortex, paracortex and medulla, or to subdivide the system of sinuses into marginal, trabecular and medullary divisions. The lymphoreticular cords contain avian germinal centers (AGC) with B-lymphocytes and the area of T-lymphocytes. Postcapillary venules are responsible for the recirculation of lymphocytes. Sinus reticular cells do not exist in the ALN, but free macrophages are present. The phagocytic capacity of the macrophages was determined by injection of vital dyes (India ink, Berlin blue) and inoculation with Candida cells. Macrophages filled with markers migrate from the lymph sinuses into the lymphoreticular cords and further into the AGC. The mobility of the macrophages is remarkably lower after phagocytosis of Candida cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00204887
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of secretory vesicles in homogenates of pea stem segments (1983)
    Springer
    Planta 158 (1983), S. 534-539 
    Details
    ISSN: 1432-2048
    Keywords: Glucan synthase ; Golgi apparatus ; Inosine diphosphatase ; Pisum, secretory vesicles ; Renografin gradient ; Secretory vesicle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In homogenates of stem sections from etiolated pea (Pisum sativum L.) seedlings, secretory vesicles can be separated from Golgi-apparatus cisternae by rate-zonal centrifugation in renografin gradients. Optically, two bands of turbidity are observed, the uppermost containing the secretory vesicles and the lower one the Golgi-apparatus cisternae. The absence of glutaraldehyde in the homogenizing medium has allowed the effective characterization of marker-enzyme activities. Golgi-apparatus cisternae have been recognized by the presence of inosine-diphosphatase and glucan-synthase I activities as well as by electron microscopy. In contrast, although secretory vesicles also bear inosine diphosphatase they do not appear to possess glucan-synthase activity. Three plasma-membrane markers, NPA-binding, glucan synthase II, and KCl,Mg2+-adenosine triphosphatase (pH 6.5), were not detected in secretory vesicles. Pulse-chase experiments with [3H]glucose support our designation of secretory vesicles and Golgi-cisternal fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397244
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  • 4
    Electronic Resource
    Electronic Resource
    Electron microscopy of extracellular materials during the development of a sea star, Patiria miniata (Echinodermata: Asteroidea) (1983)
    Springer
    Cell & tissue research 234 (1983), S. 193-200 
    Details
    ISSN: 1432-0878
    Keywords: Sea star ; Development ; Cuticle ; Extracellular materials ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of conspicuous extracellular materials during the life history of a sea star (Patiria miniata) is described. The outer surface of the developing sea star is covered by two morphologically different cuticles that appear sequentially during ontogeny. The primary cuticle, which is about 120 nm thick and two-layered, is present from mid-blastula through the end of the larval stage. The secondary cuticle, which is about 1 μm thick and three-layered, first appears on the epidermis of the rudiment region of the larva and, after metamorphosis, covers the entire epidermis of the juvenile and adult stages. During ontogeny, there are only two conspicuous gut cuticles: the first lines the newly invaginated archenteron at the start of the gastrula stage, and the second lines the esophagus during the larval stage. A blastocoelic basal lamina first appears at mid-blastula and persists as subectodermal and subendodermal basal laminae. Ruthenium red-positive granules are detectable between the lateral surfaces of adjacent ectodermal cells during part of the gastrula stage; this transient intercellular material may possibly aid in lateral adhesion between cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217412
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  • 5
    Electronic Resource
    Electronic Resource
    Laminated figures of the intercisternal regions of dictyosome-like structures from guinea-pig spermatocytes fixed with glutaraldehyde-tannic acid (1983)
    Springer
    Cell & tissue research 234 (1983), S. 633-639 
    Details
    ISSN: 1432-0878
    Keywords: Dictyosome-like structures ; Golgi apparatus ; Phosphatidylcholine ; Laminated figures ; Electron microscopy ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dictyosome-like structures (DLS) of guinea pig spermatocytes, when prefixed in mixtures of glutaraldehyde and tannic acid, exhibited laminated figures with a repeating periodicity of about 4.5 nm in the spaces between DLS saccules or in association with the surfaces of the DLS saccules. These laminated figures were similar to those figures derived from saturated lipids in other tissues. Alternatively, spaces between saccules were collapsed leaving only thin, electron-dense material separating adjacent saccules. These changes were not observed when the DLS were prefixed in glutaraldehyde before exposure to tannic acid. The presence of laminated figures following fixation with tannic acid and osmium tetroxide suggests that saturated lipids are present in, or associated with, the intersaccular regions of the DLS. The distribution of laminated figures in other membrane structures was not affected by post fixation with tannic acid nor were laminated figures comparable to those of the DLS observed between cisternae of the Golgi apparatus. These results support previous conclusions that DLS are distinct from Golgi apparatus and are a unique component of the germ cell cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218656
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  • 6
    Electronic Resource
    Electronic Resource
    Monensin affects the trans half ofEuglena dictyosomes (1983)
    Springer
    Protoplasma 114 (1983), S. 119-124 
    Details
    ISSN: 1615-6102
    Keywords: Monensin ; Ionophore ; Euglena ; Golgi apparatus ; Dictyosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Euglena gracilis was treated in 10-5 M monensin for various times from 2 minutes to 24 hours, and then processed for electron microscopy by fixation in glutaraldehyde and osmium tetroxide or potassium permanganate. Monensin affected the mature (trans) half of the cisternae but not the forming (cis) half of the cisternae. After glutaraldehyde and osmium tetroxide fixation, the affected cisternae appeared swollen, whereas after potassium permanganate fixation, the affected cisternae were distorted but not swollen. The monensin effect was first noticeable after 5 minutes of treatment and the maximum effect was observed after only 10 minutes of treatment. No additional monensin effects were observed up to 24 hours of treatment; however, by 24 hours there was variability in dictyosome form and some dictyosomes appeared relatively normal. The first noticeable effects at the 5 minute treatment time involved either the most mature (trans) cisterna or cisternae in the middle of the stack. Thus, inEuglena, the region of the Golgi apparatus that responds to monensin by cisternal dilation is restricted to the mature (trans) half of the dictyosomes, with the initial response given by specific cisternae in the stack.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279875
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  • 7
    Electronic Resource
    Electronic Resource
    The Golgi apparatus of ciliated cells in the cat trachea negatively-stainedin situ and in cell fractions (1983)
    Springer
    Protoplasma 115 (1983), S. 193-201 
    Details
    ISSN: 1615-6102
    Keywords: Golgi apparatus ; Negative staining ; Tannic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Application of a modified tannic acid fixation and en bloc staining technique to specimens of cat trachea resulted in an unusual condition of electron-contrast, with a dense cytosol and relatively unstained membrane-bound organelles, in effect producing a negatively-stained image in thin sections. Thus, a direct comparison was possible between negatively-stained Golgi apparatusin situ and Golgi apparatus isolated from homogenates of the same tissue and negatively-stained with phosphotungstic acid. In both instances, these organelles revealed closely similar, if not identical, cisternal features including solid or fenestrated central saccules, often with fenestrated peripheries, and a system of peripheral cisternal tubules. Our findings provide directin situ validation of the general pattern of Golgi apparatus architecture first revealed by isolated and negatively-stained Golgi apparatus preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279809
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  • 8
    Electronic Resource
    Electronic Resource
    Coated vesicles of mouse liver are surrounded by a zone of exclusion (1983)
    Springer
    Protoplasma 116 (1983), S. 86-89 
    Details
    ISSN: 1615-6102
    Keywords: Coated vesicles ; Golgi apparatus ; Zone of exclusion ; Liver (mouse)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spiny-(clathrin-)coated vesicles of rodent liver, both at the mature or trans face of the Golgi apparatus and at the cell surface, as well as coated pits, are surrounded by a “halo” or zone of exclusion that extends 55–75 nm beyond the membrane of the coated vesicle. The existence of a zone of exclusion surrounding vesicles close to the sinusoidal membrane and within the region of the Golgi apparatus coincides with the appearance of a spiny network on the cytoplasmic surface of these vesicles. This property is not exhibited by transition vesicles at the immature or cis face of the Golgi apparatus and serves to further distinguish these transition vesicles from the 60–80 nm diameter spiny-coated microvesicles found primarily at the mature Golgi apparatus face.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01294235
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  • 9
    Electronic Resource
    Electronic Resource
    Some observations on the development of dictyosome-like structures in guinea pig testes (1983)
    Springer
    Protoplasma 116 (1983), S. 187-197 
    Details
    ISSN: 1615-6102
    Keywords: Testes ; Dictyosome-like structures ; Mammalian reproduction ; Golgi apparatus ; Spermatocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Structures superficially resembling dictyosomes (DLS) are present in guinea pig spermatocytes. They are first visible in late stages of spermatogonial development as single, irregularly shaped saccules. DLS saccules continue to form, at what appears to be a much accelerated rate, during the first stages of spermatocyte development. After formation, the saccules mature and aggregate into a stacked, or dictyosomal, configuration. DLS reach their maximum numbers in spermatocytes just prior to the formation of proacrosomal granules and, at this time, constitute more membrane than Golgi apparatus and as much as 25% of the total endomembrane of the spermatocyte. The DLS then decline in numbers and only a few remain in spermatids. DLS reappear just prior to spermiation and become conspicuous features of the residual body and cytoplasmic droplet. DLS membranes have structural and cytochemical similarities to the membranes of the mature (trans) faces of the Golgi apparatus, and especially the thick cisternae of the spermatid Golgi apparatus. They are similar, also, to the membranes of the acrosome, the cell surface, multivesicular bodies (MVB), and a class of vesicles whose membranes appear “thin” following fixation in tannic acid. Both the thin membrane vesicles (i.e., the tannic acid asymmetrical vesicles-TAAV) and the MVB may act as membrane pools for the generation and/or dissipation of DLS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279837
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  • 10
    Electronic Resource
    Electronic Resource
    Three classes of spiny-(Clathrin-) coated vesicles in rodent liver based on size distributions (1983)
    Springer
    Protoplasma 117 (1983), S. 45-52 
    Details
    ISSN: 1615-6102
    Keywords: Coated vesicles ; Clathrin ; Cell surface ; Golgi apparatus ; Membrane recycling ; Liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Clathrin-coated vesicles in rodent (rat and mouse) liver distribute into three distinct populations based on measurements of vesicle diameter. The first population consists of 60–80 nm vesicles found almost exclusively within the Golgi apparatus region. The second population is of 100–160 nm coated vesicles located within 100–500 nm of the cell surface. A third population of coated vesicles of intermediate diameter (ca. 90 nm) is present both at the Golgi apparatus and at the cell surface. We speculate that clathrin and clathrin-coated vesicles within the region of the Golgi apparatus and of the cell surface exist in two recycling populations. The third population of vesicles of intermediate diameter could represent a shuttle to link the two major compartments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281783
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