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  • LIFE SCIENCES (GENERAL)  (3)
  • 2010-2014
  • 1980-1984  (3)
  • 1975-1979
  • 1970-1974
  • 2011
  • 1983  (3)
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  • 2010-2014
  • 1980-1984  (3)
  • 1975-1979
  • 1970-1974
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  • 1
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    Stabilization of the yeast desaturase system by low levels of oxygen (1983)
    In:  Other Sources
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    Publication Date: 2011-08-18
    Description: The stability of particulate palmitoyl-CoA desaturase preparations from anaerobically grown yeast cells was increased by exposure to low levels of oxygen. The stabilizing effect of oxygen may be based upon the increased amounts of palmitoleic acid and ergosterol that become available to the cells. These results suggest the evolutinary appearance of this system at a time when atmospheric oxygen was at a low level.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Origins of Life (ISSN 0302-1688); 13; March 19
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    NASA TECHNICAL REPORTS
  • 2
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    Oxygen requirements for formation and activity of the squalene expoxidase in Saccharomyces cerevisiae (1983)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: The effect of oxygen on squalene epoxidase activity in Saccharomyces cerevisiae was investigated. In cells grown in standing cultures, the epoxidase was localized mainly in the 'mitochondrial' fraction. Upon aeration, enzyme activity increased and the newly formed enzyme was associated with the 'microsomal' fraction. At 0.03 percent (vol/vol) oxygen, epoxidase levels doubled, whereas the ergosterol level was only slightly increased. Cycloheximide inhibited the increase in epoxidase under these conditions. An apparent K sub m for oxygen of 0.38 percent (vol/vol) was determined from a crude particulate preparation for the epoxidase.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Journal of Bacteriology (ISSN 0021-9193); 488-492
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    NASA TECHNICAL REPORTS
  • 3
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    Biochemical changes in rat liver after 18.5 days of spaceflight (41566) (1983)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: The effect of weightlessness on liver metabolism was investigated using tissue from rats flown in earth orbit for 18.5 days on the Soviet Cosmos 936 biosatellite and the changes in the activities of 28 carbohydrate and lipid enzymes were determined. The activities of two enzymes, palmitoyl-CoA desaturase and lactate dehydrogenase, increased, while the activities of five, glycogen phosphorylase, 6-phosphogluconate dehydrogenase, both acyltransferases which act on alpha-glycerolphosphate and diglycerides, and and aconitate hydratase decreased. The other enzyme activities were found to be unchanged. In addition, increased levels of liver glycogen and palmitoleate were detected which probably resulted from the lowered glycogen phosphorylase and increased palmitoyl-CoA desaturase activities, respectively, in those animals that experienced weightlessness. All of the changes observed in the rats after 18.5 days of spaceflight disappear by 25 days after the flight.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Society for Experimental Biology and Medicine, Proceedings (ISSN 0037-9727); 172; 1983
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    NASA TECHNICAL REPORTS
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