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  • EARTH RESOURCES AND REMOTE SENSING  (308)
  • Physics  (134)
  • FLUID MECHANICS AND HEAT TRANSFER  (130)
  • Life and Medical Sciences  (129)
  • COMMUNICATIONS AND RADAR  (119)
  • 1980-1984  (820)
  • 1940-1944
  • 1920-1924
  • 1984  (395)
  • 1983  (425)
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  • 1980-1984  (820)
  • 1940-1944
  • 1920-1924
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  • 1
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-07-13
    Description: The use of satellite images obtained from various dates is essential for crop forecast systems. In order to make possible a multitemporal analysis, it is necessary that images belonging to each acquisition have pixel-wise correspondence. A system developed to obtain, register and record image segments from LANDSAT images in computer compatible tapes is described. The translational registration of the segments is performed by correlating image edges in different acquisitions. The system was constructed for the Burroughs B6800 computer in ALGOL language.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: E83-10373 , NASA-CR-172917 , NAS 1.26:172917 , INPE-2785-PRE/351 , Encontro Nacl. de Automatica Soc. Brasil. de Mat.; Jul 06, 1983 - Jul 13, 1983; Belem, Para
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  • 3
    Publication Date: 2011-08-19
    Description: An experiment using the Shuttle Imaging Radar-B (SIR-B) to monitor certain properties of the ocean wave directional spectrum and to track the long swell systems as they propagate northward to encounter the Agulhas near the southeastern coast of Africa is discussed. The experiment is designed around the unique capability of SIR-B to overcome key limitations of the Seasat synthetic aperture radar data set, and to extend the existing Seasat results into new areas. Ocean wave systems will be tracked. The variable-incidence-angle capability to examine wave imaging quality will be utilized. Doppler current measurements will be attempted. An effort will be made to verify that the lower range-to-velocity ratio of SIR-B will lead to the improved response of azimuth-traveling wave systems.
    Keywords: COMMUNICATIONS AND RADAR
    Type: JPL The SIR-B Sci. Invest. Plan; 4 p
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  • 4
    Publication Date: 2011-08-18
    Description: Multitemporal Thematic Mapper, Thematic Mapper Simulator, and detailed ground truth data were collected for a 9- by 11-km sample segment in Webster County, IA, in the summer of 1982. Three dates were acquired each with Thematic Mapper Simulator (June 7, June 23, and July 31) and Thematic Mapper (August 2, September 3, and October 21). The Thematic Mapper Simulator data were converted to equivalent TM count values using TM and TMS calibration data and model based estimates of atmospheric effects. The July 31, TMS image was compared to the August 2, TM image to verify the conversion process. A quantitative measure of proportion estimation variance (Fisher information) was used to evaluate the corn/soybeans separability for each TM band as a function of time during the growing season. The additional bands in the middle infrared allowed corn and soybeans to be separated much earlier than was possible with the visible and near-infrared bands alone. Using the TM and TMS data, temporal profiles of the TM principal components were developed. The greenness and brightness exhibited behavior similar to MSS greenness and brightness for corn and soybeans.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: IEEE Transactions on Geoscience and Remote Sensing (ISSN 0196-2892); GE-22; 312-318
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  • 5
    Publication Date: 2016-06-07
    Description: The 250-2500 km baseline vectors between radio telescopes located at Tidbinbilla (DSS43) near Canberra, Parkes, Fleurs (X3) near Sydney, Hobart and Alice Springs were determined from radio interferometric observations of extragalactic sources. The observations were made during two 24-hour sessions on 26 April and 3 May 1982, and one 12-hour night-time session on 28 April 1982. The 275 km Tidbinbilla - Parkes baseline was measured with an accuracy of plus or minus 6 cm. The remaining baselines were measured with accuracies ranging from 15 cm to 6 m. The higher accuracies were achieved for the better instrumented sites of Tidbinbilla, Parkes and Fleurs. The data reduction technique and results of the experiment are discussed.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: The Telecommun. and Data Acquisition Rept.; p 140-146
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  • 6
    Publication Date: 2019-06-28
    Description: A two-dimensional monolithic array has been developed that gives the elevation and azimuth of point source targets. The array is an arrangement of rows and columns of antennas and bismuth bolometer detectors on a fused quartz substrate. Energy is focused onto the array through a lens placed on the back side of the substrate. At 1.38 mm with a 50 mm diameter objective lens, the array has demonstrated a positioning accuracy of 26 arcmin. In a differential mode this precision improves to 9 arcsec, limited by the mechanics of the rotating stage. This tracking could be automated to a fast two-step procedure where a source is first located to the nearest row and column, and then precisely located by scanning. With signal processing the array should be able to track multiple sources.
    Keywords: COMMUNICATIONS AND RADAR
    Type: IEEE Transactions on Antennas and Propagation (ISSN 0018-926X); AP-31; May 1983
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  • 7
    Publication Date: 2019-06-28
    Description: The techniques applied to MSS-LANDSAT data in the land-use mapping of Criciuma region (Santa Catarina state, Brazil) are presented along with the results of a classification accuracy estimate tested on the resulting map. The MSS-LANDSAT data digital processing involves noise suppression, features selection and a hybrid classifier. The accuracy test is made through comparisons with aerial photographs of sampled points. The utilization of digital processing to map the classes agricultural lands, forest lands and urban areas is recommended, while the coal refuse areas should be mapped visually.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: E85-10017 , NASA-CR-168574 , NAS 1.26:168574 , INPE-2945-RPE/447
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  • 8
    Publication Date: 2019-06-28
    Description: (Previously cited in issue 06, p. 860, Accession no. A82-17824)
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
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  • 9
    Publication Date: 2019-06-28
    Description: The utilization of remote sensing data for monitoring land use changes by means of digital image analysis is described. The following data were utilized: LANDSAT data from September 4, 1975, April 24, 1978, and September 8, 1981; LANDSAT paper photography data; area IV color photographs; IBGE topography maps, and auxiliary data about the Brazilian state of Santa Catarina. Three kinds of analyses of digital images were carried out. The project identified and mapped major classes of land use areas including urban areas, coal deposits, agricultural areas, forests, lakes, and flood plains. Five areas directly affected by coal exploration southeast of Santa Catarina are identified and described. In addition, the classification system used for organizing data about land cover in a hierarchical arrangement is presented. The project made use of two remote sensing data sources: data of MSS spectral (Mulitspectral Scanner System)/LANDSAT on a scale of 1:100,000 with approximately 80 m resolution, and infrared color aerial photographs on a scale of 1:45,000 with approximately 5 m resolution. Therefore, the classification system included three levels, two selected to be compatible with aerial photography data and the third to conform to the resolution of MSS/LANDSAT.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: NASA-CR-173946 , NAS 1.26:173946 , INPE-2874-NTE/204
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  • 10
    Publication Date: 2019-06-28
    Description: Progress on defining the minimum Landsat-4 data characteristics needed for agricultural information in the U.S. and assessing the value-added capability of current technology to extract that level of information is reported. Emphasis is laid on the thematic mapper (TM) data and the ground processing facilities. TM data from all 7 bands for a rural Arkansas scene were examined in terms of radiometric, spatial, and geometric fidelity characteristics. Another scene sensed over Iowa was analyzed using three two-channel data sets. Although the TM data were an improvement over MSS data, no value differential was perceived. However, the development of further analysis techniques is still necessary to determine the actual worth of the improved sensor capabilities available with the TM, which actually has an MSS within itself.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: AAS PAPER 83-160
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