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  • Golgi apparatus  (9)
  • Pisum
  • Springer  (13)
  • Annual Reviews
  • Springer Science + Business Media
  • 1980-1984  (13)
  • 1975-1979
  • 1984  (4)
  • 1983  (9)
Collection
Keywords
Publisher
  • Springer  (13)
  • Annual Reviews
  • Springer Science + Business Media
Years
  • 1980-1984  (13)
  • 1975-1979
Year
  • 1
    Electronic Resource
    Electronic Resource
    Influence of winter and summer growth conditions on leaf membrane lipids of Pisum sativum L. (1983)
    Springer
    Planta 157 (1983), S. 218-223 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast membranes ; Fatty-acid composition (leaf membranes) ; Pisum ; Seasonal variation (fatty acids) ; Thylakoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lipid composition and degree of unsaturation of fatty acids has been measured for membranes isolated from leaves of Pisum sativum grown under either summer or winter conditions. Although the lipid-class composition was not significantly changed for the two growth conditions the degree of unsaturation of the fatty acids was greater for winter than summer plants. The difference in unsaturation was evident with all lipid classes of the non-thylakoid membranes including the galactolipids of the chloroplast envelope. In contrast, both the relative amounts of lipid classes and degree of saturation were not greatly changed for summer and winter thylakoids with the exception that phosphatidylglycerol had a greater linolenic acid (18:3) content for the thylakoids of winter grown leaves. However, a striking difference was found for the total acyl lipid to chlorophyll ratio for thylakoids isolated from summer or winter plants, with the former producing a lower ratio than the latter growth conditions. The above changes in lipid composition of chloroplast membranes are discussed in terms of optimizing their functional activities under the different growth conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405185
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of secretory vesicles in homogenates of pea stem segments (1983)
    Springer
    Planta 158 (1983), S. 534-539 
    Details
    ISSN: 1432-2048
    Keywords: Glucan synthase ; Golgi apparatus ; Inosine diphosphatase ; Pisum, secretory vesicles ; Renografin gradient ; Secretory vesicle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In homogenates of stem sections from etiolated pea (Pisum sativum L.) seedlings, secretory vesicles can be separated from Golgi-apparatus cisternae by rate-zonal centrifugation in renografin gradients. Optically, two bands of turbidity are observed, the uppermost containing the secretory vesicles and the lower one the Golgi-apparatus cisternae. The absence of glutaraldehyde in the homogenizing medium has allowed the effective characterization of marker-enzyme activities. Golgi-apparatus cisternae have been recognized by the presence of inosine-diphosphatase and glucan-synthase I activities as well as by electron microscopy. In contrast, although secretory vesicles also bear inosine diphosphatase they do not appear to possess glucan-synthase activity. Three plasma-membrane markers, NPA-binding, glucan synthase II, and KCl,Mg2+-adenosine triphosphatase (pH 6.5), were not detected in secretory vesicles. Pulse-chase experiments with [3H]glucose support our designation of secretory vesicles and Golgi-cisternal fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397244
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  • 3
    Electronic Resource
    Electronic Resource
    Pollen irradiation and the transfer of maternal genes in Pisum sativum (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 245-248 
    Details
    ISSN: 1432-2242
    Keywords: Pollen ; Irradiation ; Gene transfer ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pollen of Pisum sativum was exposed to doses of 900 to 6,000 r of X-rays prior to pollinating a multiply marked genotype. The first generation progeny closely resembled that produced with unirradiated pollen. In the second generation, five loci were monitored, and the results showed that irradiation enhanced the proportion of maternal information transmitted to the progeny; the practical implications of the data, as well as the mechanism underlying the effect are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317046
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  • 4
    Electronic Resource
    Electronic Resource
    Inheritance of legumin and albumin contents in a cross between round and wrinkled peas (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 101-107 
    Details
    ISSN: 1432-2242
    Keywords: Speed proteins ; Legumin ; Albumin ; Heritability ; Pisum ; Pea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Legumin and albumin are the fractions of pea seed proteins preferred to vicilin because of their high sulfur amino acid contents. The joint inheritance of legumin and albumin contents was studied in a cross between to contrasting lines of peas — one with high legumin and low albumin, and the other with low legumin and medium to high albumin. Single seed determinations were made in the parental, F1; F2 and backcross generations using rocket immunoelectrophoresis. In the non-segregating generations (P1, P2 and F1), legumin and albumin contents were negatively correlated (r=−≅0.50). The estimates of correlation coefficients in the segregating generations (F2, BC1 and BC2) were also about −0.5. However, the two estimates based on the round and on the wrinkled seeds separately in the F2 generation were not significantly different from zero. At least four individual round F2 seeds showed the desired recombination of high legumin with high albumin indicating that the unfavorable correlation can be broken. In this cross legumin content showed predominantly additive genetic variation whereas the dominance variance was the largest component for albumin content. A combined “relative sulfur index”, proposed as a convenient measure for selection, showed a narrow sense heritability of 47%. In general these results support the view that sulfur amino acid content of peas can be improved by breeding, but that the required selection regime must take both legumin and albumin content into account.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252322
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  • 5
    Electronic Resource
    Electronic Resource
    Laminated figures of the intercisternal regions of dictyosome-like structures from guinea-pig spermatocytes fixed with glutaraldehyde-tannic acid (1983)
    Springer
    Cell & tissue research 234 (1983), S. 633-639 
    Details
    ISSN: 1432-0878
    Keywords: Dictyosome-like structures ; Golgi apparatus ; Phosphatidylcholine ; Laminated figures ; Electron microscopy ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dictyosome-like structures (DLS) of guinea pig spermatocytes, when prefixed in mixtures of glutaraldehyde and tannic acid, exhibited laminated figures with a repeating periodicity of about 4.5 nm in the spaces between DLS saccules or in association with the surfaces of the DLS saccules. These laminated figures were similar to those figures derived from saturated lipids in other tissues. Alternatively, spaces between saccules were collapsed leaving only thin, electron-dense material separating adjacent saccules. These changes were not observed when the DLS were prefixed in glutaraldehyde before exposure to tannic acid. The presence of laminated figures following fixation with tannic acid and osmium tetroxide suggests that saturated lipids are present in, or associated with, the intersaccular regions of the DLS. The distribution of laminated figures in other membrane structures was not affected by post fixation with tannic acid nor were laminated figures comparable to those of the DLS observed between cisternae of the Golgi apparatus. These results support previous conclusions that DLS are distinct from Golgi apparatus and are a unique component of the germ cell cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218656
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution of golgi apparatus-associated polyribosomes across the polarity axis of dictyosomes of wild carrot (Daucus carota L.) (1984)
    Springer
    Protoplasma 123 (1984), S. 221-225 
    Details
    ISSN: 1615-6102
    Keywords: Golgi apparatus ; Endoplasmic reticulum ; Polyribosomes ; Golgi apparatus-associated polyribosomes ; Wild carrot (Daucus carota)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Endoplasmic reticulum-polyribosome-Golgi apparatus associations were a general feature of cells of suspension cultures of wild carrot (Daucus carota L.). Free polyribosomes occurred within the Golgi apparatus zone for all dictyosomes and with equal frequency at all levels within the stack including the most mature or trans face. When evaluated and quantified from electron micrographs, approximately 60% of the dictyosome profiles were characterized by a system of transition elements consisting of part smooth-part rough endoplasmic reticulum. These were encountered most frequently in the immediate vicinity of the immature, forming or cis face, usually toward the periphery of the stacked cisternae. Analysis of serial sections showed that those dictyosome profiles not exhibiting this characteristic did so primarily because of an unfavorable plane of sectioning. All dictyosomes examined in 5 or more serial sections revealed some type of close association with endoplasmic reticulum. Some of the associations were so close that direct connections between Golgi apparatus and endoplasmic reticulum tubules could not be excluded. Also present, especially at the forming or cis face, were small 600 nm transition vesicles with nap-like surface coats on nearly 90% of the dictyosomes examined. More than 50% exhibited spiny (clathrin-)coated vesicles at the mature or trans face.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281169
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  • 7
    Electronic Resource
    Electronic Resource
    Monensin affects the trans half ofEuglena dictyosomes (1983)
    Springer
    Protoplasma 114 (1983), S. 119-124 
    Details
    ISSN: 1615-6102
    Keywords: Monensin ; Ionophore ; Euglena ; Golgi apparatus ; Dictyosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Euglena gracilis was treated in 10-5 M monensin for various times from 2 minutes to 24 hours, and then processed for electron microscopy by fixation in glutaraldehyde and osmium tetroxide or potassium permanganate. Monensin affected the mature (trans) half of the cisternae but not the forming (cis) half of the cisternae. After glutaraldehyde and osmium tetroxide fixation, the affected cisternae appeared swollen, whereas after potassium permanganate fixation, the affected cisternae were distorted but not swollen. The monensin effect was first noticeable after 5 minutes of treatment and the maximum effect was observed after only 10 minutes of treatment. No additional monensin effects were observed up to 24 hours of treatment; however, by 24 hours there was variability in dictyosome form and some dictyosomes appeared relatively normal. The first noticeable effects at the 5 minute treatment time involved either the most mature (trans) cisterna or cisternae in the middle of the stack. Thus, inEuglena, the region of the Golgi apparatus that responds to monensin by cisternal dilation is restricted to the mature (trans) half of the dictyosomes, with the initial response given by specific cisternae in the stack.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279875
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  • 8
    Electronic Resource
    Electronic Resource
    The development of specific sieve-element plastids in wound phloem ofColeus blumei (S-type) andPisum sativum (P-type), regenerated from amyloplast-containing parenchyma cells (1983)
    Springer
    Protoplasma 114 (1983), S. 125-132 
    Details
    ISSN: 1615-6102
    Keywords: Sieve-element plastids ; Wound phloem ; Regeneration ; Sieve-tube starch ; Coleus ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In experimentally-induced wound phloem, sieve-element plastids express their genetically determined type in depositing amylopectinrich sieve-tube starch (Coleus, S-type) and polygonal protein crystals (Pisum, P-type). Sieve-element plastids budd off from preexisting amyloplasts, pass through a short amoeboid state and develop into spherical plastids with translucent matrix. During early phases of differentiation wound sieve-elements contain two populations of plastids: typical sieve-element plastids and residual parenchyma plastids with large amylose-rich starch grains. The retardation in the break down of the latter is discussed. Sieve-tube and amyloplast starches are likewise digested by α-1,4- and α-1,6-bond cleaving glucosidases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279876
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  • 9
    Electronic Resource
    Electronic Resource
    The Golgi apparatus of ciliated cells in the cat trachea negatively-stainedin situ and in cell fractions (1983)
    Springer
    Protoplasma 115 (1983), S. 193-201 
    Details
    ISSN: 1615-6102
    Keywords: Golgi apparatus ; Negative staining ; Tannic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Application of a modified tannic acid fixation and en bloc staining technique to specimens of cat trachea resulted in an unusual condition of electron-contrast, with a dense cytosol and relatively unstained membrane-bound organelles, in effect producing a negatively-stained image in thin sections. Thus, a direct comparison was possible between negatively-stained Golgi apparatusin situ and Golgi apparatus isolated from homogenates of the same tissue and negatively-stained with phosphotungstic acid. In both instances, these organelles revealed closely similar, if not identical, cisternal features including solid or fenestrated central saccules, often with fenestrated peripheries, and a system of peripheral cisternal tubules. Our findings provide directin situ validation of the general pattern of Golgi apparatus architecture first revealed by isolated and negatively-stained Golgi apparatus preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279809
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  • 10
    Electronic Resource
    Electronic Resource
    Coated vesicles of mouse liver are surrounded by a zone of exclusion (1983)
    Springer
    Protoplasma 116 (1983), S. 86-89 
    Details
    ISSN: 1615-6102
    Keywords: Coated vesicles ; Golgi apparatus ; Zone of exclusion ; Liver (mouse)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spiny-(clathrin-)coated vesicles of rodent liver, both at the mature or trans face of the Golgi apparatus and at the cell surface, as well as coated pits, are surrounded by a “halo” or zone of exclusion that extends 55–75 nm beyond the membrane of the coated vesicle. The existence of a zone of exclusion surrounding vesicles close to the sinusoidal membrane and within the region of the Golgi apparatus coincides with the appearance of a spiny network on the cytoplasmic surface of these vesicles. This property is not exhibited by transition vesicles at the immature or cis face of the Golgi apparatus and serves to further distinguish these transition vesicles from the 60–80 nm diameter spiny-coated microvesicles found primarily at the mature Golgi apparatus face.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01294235
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