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  • 1
    Electronic Resource
    Electronic Resource
    Effects of hormones and amino acid depletion on the kinetic parameters of amino acid uptake in monolayer cultures of rat hepatocytes (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 67-75 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Uptake of 2-aminoisobutyric acid (AIB) at concentrations of 0.1 mM to 30 mM was examined in sodium-containing and sodium-free media in hepatocytes pretreated without hormones (control), with hormones, or with amino acid depletion. Results show that 1-minute but not 4-minute rates can be taken as initial rates for the total or sodium-dependent transport of AIB. The data for the 1-minute sodium-dependent transport of AIB were analyzed by a comptuer program and also by Eadie-Hofstee and Lineweaver-Burk plots, and a single saturable system was found. In the control cultures, the saturable system had a Km of 1-2 mM AIB and a Vmax of 1.2 nmoles AIB/mg protein/minute. There was an increase in the Vmax of two to three-fold after pretreating the cultures with insulin or amino acid depletion, three to four-fold with glucagon, and six to seven fold with glucagon + dexamethasone.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041120111
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    Paper (German National Licenses)
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  • 2
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    Plasmodium falciparum gametocytes from culture in vitro develop to sporozoites that are infectious to primates (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-09-10
    Description: Gametocytes of two strains of the human malaria parasite Plasmodium falciparum have been produced in high density by means of a continuous-flow cultivation system. The gametocytes of these two strains infected a mean of 36 percent and 71 percent, respectively, of Anopheles freeborni mosquitoes that fed on a suspension of red blood cells containing the culture gametocytes. Sporozoites harvested from the infected mosquito salivary glands were infective to the chimpanzee (Pan troglodytes) and the owl monkey (Aotus trivirgatus).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Campbell, C C -- Collins, W E -- Nguyen-Dinh, P -- Barber, A -- Broderson, J R -- New York, N.Y. -- Science. 1982 Sep 10;217(4564):1048-50.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7051285" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Anopheles/parasitology ; Aotus trivirgatus/parasitology ; Blood/parasitology ; Cells, Cultured ; Culture Media ; Humans ; Pan troglodytes/parasitology ; Plasmodium falciparum/*growth & development ; Salivary Glands/parasitology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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