Publication Date:
1982-10-15
Description:
The biotin-binding site on avidin has been labeled with biotin conjugated to undecagold, an organometallic cluster compound containing 11 gold atoms in a core angestroms in diameter. Examination of unstained specimens by scanning transmission electron microscopy reveals the labeled sites directly, without computational averaging or filtering of the images. This approach should be widely applicable for determining the locations of subunits and functional site in biological macromolecules at a resolution at a resolution in range of 15 angstroms.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Safer, D -- Hainfeld, J -- Wall, J S -- Reardon, J E -- AM 28607/AM/NIADDK NIH HHS/ -- GM 12202/GM/NIGMS NIH HHS/ -- GM 28750/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1982 Oct 15;218(4569):290-1.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7123234" target="_blank"〉PubMed〈/a〉
Keywords:
Avidin/*metabolism
;
Binding Sites
;
Biotin/*metabolism
;
Gold/*metabolism
;
Organogold Compounds
;
Organometallic Compounds/*metabolism
;
Ovalbumin/*analogs & derivatives
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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