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  • Articles  (13)
  • Springer  (13)
  • 1980-1984  (13)
  • 1975-1979
  • 1984  (6)
  • 1982  (7)
  • Medicine  (13)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 40 (1984), S. 557-559 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The presence of 10−3 M ouabain or furosemide in the perfusate inhibited saliva secretion induced by either isoproterenol (10−5 M) or phenylephrine (10−5 M) from isolated rat submandibular glands and caused characteristic alterations in the electrolyte composition of saliva.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 80 (1982), S. 147-155 
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A systematic study on autolysis of the cell walls of fungi has been made on Neurospora crassa, Botrytis cinerea, Polystictus versicolor, Aspergillus nidulans, Schizophyllum commune, Aspergillus niger, and Mucor mucedo. During autolysis each fungus produces the necessary lytic enzymes for its autodegradation. From autolyzed cultures of each fungus enzymatic precipitates were obtained. The degree of lysis of the cell walls, obtained from non-autolyzed mycelia, was studied by incubating these cell walls with and without a supply of their own lytic enzymes. The degree of lysis increased with the incubation time and generally was higher with a supply of lytic enzymes. Cell walls from mycelia of different ages were obtained. A higher degree of lysis was always found, in young cell walls than in older cell walls, when exogenous lytic enzymes were present. In all the fungi studied, there is lysis of the cell walls during autolysis. This is confirmed by the change of the cell wall structure as well as by the degree of lysis reached by the cell wall and the release of substances, principally glucose and N-acetylglucosamine in the medium.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 78 (1982), S. 17-23 
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Certain groups of fungi share chemical structures which makes difficult the isolation and differentiation of specific antigens by the usual methods of extraction and purification. Therefore, we have oriented our studies to the immunological and biochemical characterization of differences and similarities of molecular structures from fungi, etiologic agents of systemic mycoses, hoping to establish criteria for the utilization and handling of these antigens. A deproteinized polysaccharide-protein complex (D-PPC) was isolated from Histoplasma capsulatum and Blastomyces dermatitidis. The immunological studies with humoral tests indicate a variable cross reaction between antigens of both species. In immunodiffussion systems, the reaction was specific for each species using saline solution or phosphate buffer solution, while using an agarose veronal system, the cross reactions were very evident. In addition, differences in cross reactions were obtained with immunoelectrophoresis, haemagglutination and complement fixation microtest. This variation in cross reaction responses suggest that these antigens (D-PPC) share common structures but at the same time must have some different component owned by each one of the fungi which makes them more specific than crude antigens.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Human genetics 〈Berlin〉 62 (1982), S. 250-251 
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A total of 2363 Cuban newborns were screened for genetic hemoglobin abnormalities; 2187 (92.56%) had a normal electrophoretic pattern. Of the 176 samples with abnormal electrophoretic patterns, 102 (4.32%) had hemoglobins A, F plus Bart's; 54 (2.29%) had hemoglobins A, F and S; 3 (0.13%) had hemoglobins A, F, S plus Bart's; 14(0.59%) had hemoglobins A, F and C; 1 (0.04%) had hemoglobins A, F, C and Bart's. The frequency of Hb Bart's was 4.46% in AA phenotype, 5.25% in AS, and 6.67% in AC. Two newborns were found to have rare variants. A close correlation was found between the observed and expected phenotypes, which indicates the accuracy of the diagnostic methods used. The results of all hemoglobin abnormalities were entered on the infants' hospital records. In addition, these families received genetic counseling.
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  • 5
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The electrophoretic patterns of esterase (E-1), alcohol dehydrogenase (ADH), and glutamate oxaloacetate transaminase (GOT) isozymes were studied in two Spanish populations of the lily Scilla autumnalis with B-chromosome carrying individuals. The E-1 isozyme activity appears only in those individuals with B-chromosomes. None of the bulbs free of B's show it. Five bulbs, mosaic for B-content, were identified. Electrophoretic analysis shows that these bulbs are characterised by mosaicism for E-1 isozyme activity. An analysis of individual roots by both electrophoretic and cytological methods shows that tissue mosaicism for B-content correlates with tissue mosaicism for E-1 isozyme activity. The electrophoretic analysis of different roots from bulbs heterozygous for the Est-1 locus indicates that the structural gene for E-1 is not located on the B-chromosome itself. Rather there is a derepressor effect of Bs on E-1 isozyme activity. Since ADH and GOT patterns are unaffected by the presence of B-chromosomes it is clear that they do not exhibit a generalised derepressor effect.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 45 (1982), S. 3-11 
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Depolymerization kinetics of microtubules assembled to steady-state by pod ophyllotoxin treatment show a dose-dependent effect of this mitotic poison on the net rate of microtubule disassembly. Pulse-chase experiments with microtubules at steady-state indicate that the depolymerization effect induced by superstoichiometric concentrations of podophyllotoxin relative to tubulin is polar and time-dependent, i.e. the rate of tubulin loss decreases along with the time of treatment in the presence of the drug. Under these conditions the rate of microtubule disassembly is much faster than one could expect from a unique effect of drug-tubulin complex on the microtubule assembly end. Podophyllotoxin-tubulin complex is not able to induce active depolymerization of microtubules, while free podophyllotoxin is. These results are consistent with the hypothesis that this drug acts on the microtubule assembly-disassembly process by two different mechanisms: 1) as a free drug, it actively promotes polar depolymerization of microtubules, and 2) as a drug-tubulin complex, it retards the addition of subunits into the microtubule ends.
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  • 7
    ISSN: 1432-0878
    Keywords: Pineal organ ; “Synaptic” ribbons ; “Synaptic” spherules ; Melatonin ; Circadian rhythm ; Rabbit (male)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Previous studies have shown that pineal “synaptic” ribbons and spherules may respond differently under normal and experimental conditions. It has been suggested that the increase in the number of ribbons may be a prerequisite for enhanced melatonin formation. In the present study, the number of ribbons and spherules as well as the level of serum melatonin were monitored over a 24-h period in the male rabbit, the pineal gland of which is known to contain many spherules. It was found that both the number of ribbons and the levels of serum melatonin show the typical nocturnal increase, exhibiting peaks at 02:00 and 06:00 h, respectively. There is a good correlation (R = 0.8) of the two parameters. The spherules, in contrast, show no statistically significant circadian changes in number and cannot be correlated with the levels of serum melatonin. It is concluded that ribbons and spherules may differ in function and that the ribbons may be somehow involved in the regulation of melatonin formation.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 88 (1984), S. 111-113 
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In order to determine the presence of dermatophytes in healthy skin, 200 animals from the animal house of Faculty of Medicine, U.N.A.M., were studied; these were 50 rats, 50 rabbits, 50 mice, and 50 guinea pigs. Out of these 200 animals, 29.5% had positive isolation of Trichophyton mentagrophytes, var. lacticolor. The frequency variation was: rats, 68%; rabbits, 36%; mice, 8%; and guinea pigs, 6%. Male rats and male rabbits, had the higher incidence of positives. The epidemiologic repercussion of these and the significance to use these animals in biomedical investigation is discussed.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 85 (1984), S. 167-170 
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Vaginal exudates were taken from 600 new patients of the gyneco-obstetrics outpatient clinic. Candida was isolated from 261 patients, 134 (22.3%) of which had this yeast as a component of the normal flora, and in 127 (21.2%) it was considered as a pathogen. The most frequent symptoms in the last group were vaginal discharge, erythema and pruritus. Pregnancy was the most frequent opportunistic factor, followed by the association of pregnancy and malnutrition, and anemia. Vaginal candidosis was more frequent in patients of the medium socio-economical stratum. The species of Candida isolated were C. albicans (67.1%), C. tropicalis (18.$%), C. stellatoidea (8.7%), C. pseudotropicalis (2.4%), C. parakrusei (1.6%) and C. guillermondi (0.8%).
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  • 10
    ISSN: 1573-8221
    Keywords: acetylcholine ; L-glutamate ; D-tubocurarine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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