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  • Articles  (9)
  • Chemistry  (5)
  • Life and Medical Sciences  (4)
  • 1980-1984  (9)
  • 1981  (9)
  • 1
    Electronic Resource
    Electronic Resource
    Collagen synthesis in human fibroblasts: Effects of ascorbic acid and regulation by hydrocortisone (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 109 (1981), S. 121-131 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of hydrocortisone and ascorbic acid on collagen and noncollagen protein synthesis, and on growth were examined in fibroblasts derived from normal human dermis. When the medium was supplemented with 0.28 mM ascorbic acid, the apparent rate of collagen production increased 2--3 fold over the culture cycle. Ascorbic acid also caused a small increase in the apparent rate of synthesis of noncollagen protein and an elevation in growth rate and maximum cell density. Growth was not required for the increase in collagen production since addition of ascorbate to confluent cultures induced a similar increase. Hydrocortisone (1.5 μM) blocked the ascorbate-related increase in collagen production during growth and in confluent cultures. The hormone simultaneously increased the apparent rate of noncollagen protein production and maximum cell density, suggesting that the effect on collagen synthesis was specific. Inhibition of collagen production by hydrocortisone was observed only in the presence of ascorbate, while the increase in growth and noncollagen protein production occurred in the presence and absence of the vitamin.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041090114
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  • 2
    Electronic Resource
    Electronic Resource
    Descriptive and functional anatomy of the digital vascular system of the tokay, Gekko gecko (1981)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 169 (1981), S. 293-323 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The vascular system of the digits of the tokay is more complex than has hitherto been suspected and has a configuration which suggests it is intimately involved in the process of adhesion. Distinction can be made between lamellae (expanded scales beneath the proximal portion of the digit) and scansors (elaborations of lamellae that contain a large amount of subdermal material and therefore do not technically qualify as scales). Such a distinction is made on anatomical grounds and can be appreciated only if anatomical investigation is carried out. There are no externally obvious features by which lamellae and scansors can be distinguished, except position. Scansors are found beneath hyperextensible phalanges, whereas lamellae are located more proximally and are not subjected to digital hyperextension during locomotion. Whether this distinction can be applied to other pad-bearing geckos and to anoline lizards remains to be seen.The large sinus beneath the penultimate phalanx appears to govern the pressure within the system; the reticular blood systems of the scansors appear to manifest the pressure changes with respect to the locomotor substratum. Changes in pressure within the system probably permit the overlapping scansors to comply precisely with each other and with the substratum. The presence of a system based on fluid pressure differentials means that scansors are deformable along multiple axes at any one time, thus permitting a high degree of compliance with their entire surroundings. It is probable that changes in pressure within the system promote release from the substratum as well as compliance with and attachment to it. The mechanism of control of the system awaits further investigation.The pattern of the digital vascular complex has been considered in relation to the mode of operation of the digits during locomotion. Distal drainage of the sinus is ideally suited to the activity of hyperextension of the digits. This combination permits sequential pressurizing and depressurizing of the scansors and allows the bond to be created or broken in a gradual fashion rather than in an all-or-none manner. By avoiding sudden shifts in the pattern of dynamic loading (the bond is not broken instantaneously but sequentially), the risk of transverse instability during locomotion is lessened. The increase of loading on the other feet is thus gradual. The importance of this is discussed more fully elsewhere in a consideration of the structure and function of scansors. That the digits of the tokay are hyperextended during horizontal as well as vertical locomotion (Russell, '75, p. 463) can now be rationalized not only from the point of view of protection of the setae but also because of the manner in which the vascular system of the digits functions and is drained.
    Additional Material: 42 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051690305
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  • 3
    Electronic Resource
    Electronic Resource
    Bubble nucleation studies. Part I: Formation of bubble nuclei in superheated water by bursting bubbles (1981)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 27 (1981), S. 851-853 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690270524
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  • 4
    Electronic Resource
    Electronic Resource
    Part II: Bubble entrainment by drop-formed vortex rings (1981)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 27 (1981), S. 853-856 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690270525
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  • 5
    Electronic Resource
    Electronic Resource
    A branched pore kinetic model for activated carbon adsorption (1981)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 27 (1981), S. 26-32 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A branched pore kinetic model for aqueous phase activated carbon adsorption is presented in which the carbon particle is separated into rapidly and slowly diffusing regions. The model was developed to overcome problems arising from a single rate parameter analysis and is shown to describe experimental data well. In addition to very different rates of transport in the two regions, parameters estimated by regression analysis indicated differences in the adsorptive characteristics.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690270106
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  • 6
    Electronic Resource
    Electronic Resource
    Nearest-neighbor and next-nearest-neighbor effects in the proton NMR spectra of the oligoribonucleotides ApXpG, CpXpG, CpApXpUpG, ApGpXpC, and ApGpXpCpU (1981)
    New York : Wiley-Blackwell
    Biopolymers 20 (1981), S. 1383-1398 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The proton nmr spectra of the oligoribonucleotides in the series CpXpG, ApXpG, CpApXpUpG, and ApGpXpC (X = A, G, C, and U), together with the reference compounds CpG, ApG, CpApUpG, and ApGpC, have been measured. A complete analysis of all the nonexchangeable base protons and the ribose H-1′ protons was made. The insertion of a nucleotide X into a oligoribonucleotide led to shift changes at both nearest-neighbor and next-nearest-neighbor positions, which were rationalized in terms of the shielding abilities of the various bases. The derived shielding trends in the ApGpXpC series of compounds were successfully used to predict the chemical shifts of resonances in the related ApGpXpCpU series.
    Additional Material: 13 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1981.360200703
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of dexamethasone and dibutyryl cyclic AMP on polyamine synthesizing enzymes in mouse lymphoma cells (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 106 (1981), S. 375-384 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: S49.1 Lymphoma cells were arrested in G1 phase of the cell cycle when treated with either 1 μM dexamethasone (Dex) or 0.5 mM N6, O2-dibutyryl cyclic adenosine 3′ :5′ -monophosphate (Bt2cAMP) plus 0.2 mM theophylline. However, the two agents had markedly different effects on aspects of polyamine and cyclic nucleotide metabolism within the arrested cells. Bt2cAMP had an early and pronounced inhibitory effect on ornithine decarboxylase (ODC) activity causing a decrease to 40% of control within 1 h. However, there was no significant inhibition of ODC activity in the Dex-treated cells until 4 h of exposure, at which time ODC activity was reduced to approximately 60% of the control value. Sadenosyl-L-methionine decarboxylase (SAMD) activity was reduced by both agents, Bt2cAMP having the more pronounced inhibitory effect. The activity of SAMD was reduced to 40% of control after 10 h of Dex, whereas Bt2cAMP reduced the activity to approximately 25% of control within 4 h. Intracellular polyamine pools were decreased rapidly in Dex-treated cells but not in those exposed to Bt2cAMP. Bt2cAMP decreased the amount of type I (PKI) and type II (PKII) cyclic AMP-dependent protein kinase (cAMP-PK) activity to 30% of control or less within 2 h. In contrast, Dex had very little effect on either PKI or PKII until 24 h, when cell viability was affected. The specific activity of both PKI and PKII remained significantly decreased in cells exposed to Bt2cAMP for 6 h and then resuspended in fresh medium. The rapid decrease in ODC activity in response to Bt2cAMP and the slow recovery after washout may be due to the marked decreases in total PKI and PKII activities. Dex, which had no effect on PKI and PKII specific activities, only slowly inhibited ODC activity and recovery of enzyme activity was rapid upon resuspension in fresh medium. These data further stress the importance of the maintenance of the cellular protein kinase pools in the regulation of the recovery time to growth inhibition in response to naturally occurring steroids and second messengers.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041060307
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  • 8
    Electronic Resource
    Electronic Resource
    Differential conjugation of polyamines to calf nuclear and nucleolar proteins (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 109 (1981), S. 447-452 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Nuclei and nucleoli isolated from calf liver contain acid-precipitable putrescine, spermidine and spermine conjugates. The polyamines are released upon peptide bond hydrolysis. All of the nuclear putrescine conjugate and a major portion of the polyamine conjugates are localized within the nucleolus. Nuclei and nucleoli also contain, in proportions consistent with the nucleolar/nuclear protein ratio, the putative conjugating enzyme, transglutaminase, as well as amine acceptor substrates to which radiolabeled putrescine can be conjugated by endogenous enzyme. Extraction of the isolated organelles with saline solutions of increasing ionic strength showed a differential distribution of the polyamine derivatives: all the covalently linked putrescine was associated with the less soluble components of the chromatin residue, while the spermidine and spermine conjugates were associated with several salt-extractable protein fractions as well as tightly bound to the chromatin pellet. Mono-γ-glutamyl putrescine was detected after proteolytic digestion of the 600 mM NaCl fraction, further suggesting the enzymatic action of transglutaminase(s) in the conjugation process.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041090310
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  • 9
    Electronic Resource
    Electronic Resource
    Decomposition of the [13CC2H6O]+· ion in the mass spectrum of 1-[13C]methylcyclobutanol (1981)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 16 (1981), S. 236-237 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210160510
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