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  • 1
    Electronic Resource
    Electronic Resource
    Entamoeba histolytica: Collagenolytic Activity and Virulence (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Several axenic strains of pathogenic and nonpathogenic Entamoeba histolytica were tested for their capacity to digest native radioactive type I collagen gels and to produce liver abscesses when injected into the liver of newborn hamsters. The results demonstrate that the pathogenic strains of amebas (HM1:IMSS, HM3:IMSS, HM38:IMSS, and HK9) have a collagenolytic activity that closely correlates with their in vivo capacity to produce liver lesions. The nonpathogenic isolate (Laredo) did not show collagenolytic activity and failed to produce lesions in the liver of newborn hamsters. The results also demonstrate that type I collagen obtained from rodents and cats is degraded less by amebic collagenase than is bovine collagen, which is similar to human collagen. These findings suggest that species susceptibility to invasive infection may depend, among other factors, on the characteristics of the extracellular components of host tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb02995.x
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  • 2
    Electronic Resource
    Electronic Resource
    Scanning Electron Microscopy of Attached Trophozoites of Pathogenic Entamoeba histolytica (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The surface morphology of Entamoeba histolytica trophozoites of HM 1:IMSS (axenic and monoxenic) and HK9 (axenic) strains cultured on plastic and MDCK cell substrates was examined using scanning electron microscopy (SEM). The conditions for processing trophozoites were determined by comparing the SEM observations with the morphology of living amebas examined by light microscopy. The most frequent surface differentiations in all the amebas observed with SEM were lobopodia. Round cytoplasmic projections were found in approximately half of the axenic amebas. Endocytic stomas and filopodia were more common in monoxenic cultures while the uroid was found in only 2–8% of all examined amebas. The basal surfaces of the trophozoites, involved in both attachment and cytolysis, showed no unusual features, except for the presence of a small number of short filopodia at the outer edge. No differences were found in the morphology of amebas grown on artificial and natural substrates. These observations demonstrate that there are significant quantitative differences in the surface morphology of cultured trophozoites of different strains of E. histolytica and that association with bacteria produces an increase in the relative number of surface specializations of the parasite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1983.tb05345.x
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  • 3
    Electronic Resource
    Electronic Resource
    Ground-Water Age Distribution in Madrid Basin, Spain (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 20 (1982), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: The alluvial deposits that occupy the Madrid Basin in central Spain form an aquifer system covering an area of 5,000 km2 (2,000 mi2) and with thicknesses of 2,000 m (6,600 ft) or more. Average annual precipitation is 500 mm (15 in.) and average annual temperature is 15°C (59°F). The precipitation is sufficient to provide a net surplus to ground-water recharge which, in turn, supports dry weather flow of major streams in the basin. A distribution of surface recharge and hydraulic conductivities were obtained from a previous study utilizing a two-dimensional finite-difference model of the same vertical cross section as this study. In this study a flow net and a discrete-state compartment (or “mixing-cell”) model were employed to calculate the age distribution of ground water circulating through the aquifer. Carbon-14 decay ages were determined for nine ground-water samples taken from eight locations. The ages obtained with the flow net and with the mixing-cell models are mutually consistent and generally agree with the carbon-14 decay ages. The calculated ages range from zero at the recharge boundaries to over 100,000 years at discharge boundaries in stream channels. The results obtained are to be regarded as preliminary. Their principal value will be to guide future C-14 field sampling programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1982.tb01388.x
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  • 4
    Electronic Resource
    Electronic Resource
    THE FATE OF AFLATOXINS IN THE PREPARATION OF PROTEIN CONCENTRATES AND ISOLATES FROM CONTAMINATED PEANUT AND COTTONSEED FLOURS (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 41 (1976), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With the assistance of the laboratories of six major soybean protein producers, simulated commercial protein concentrate and isolate processes were applied to aflatoxin-contaminated peanut and cottonseed flours to determine the fate of the aflatoxins. Processes for producing, concentrate by washing the flour at the protein isoelectric point (pH 4.5) were ineffective in separating the aflatoxin from the products, but a process using an aqueous alcohol wash accomplished a 90% reduction in the aflatoxin level of the concentrate compared to the flour. Processes for producing isolate by protein dissolution in alkali and its recovery by precipitation at the isoelectric point resulted in some aflatoxin loss but an increase in the aflatoxin concentration associated with the protein isolate compared to the original flour. However, a process applying carbon adsorbent to the alkaline solution accomplished a 90% reduction in the aflatoxin level of the isolate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1976.tb14435.x
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  • 5
    Electronic Resource
    Electronic Resource
    A RADIOASSAY FOR MACROPHAGES AND ITS APPLICATION FOR TESTING MACROPHAGE MEMBRANE ANTIGENS (1975)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 2 (1975), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Monolayers of mouse peritoneal macrophages actively incorporated 14C-labelled uridine, an RNA precursor. This uptake did not seem to be due to in vitro activation or stimulation of the cells but rather to their high RNA metabolic activity. 14C-uridine uptake by the unstimulated adherent cells was a characteristic property of macrophages, because it was not reduced when the cells were (i) pretreated with trypsin (to detach remaining lymphocytes), (ii) precultured for several days (to allow PMN to die off) and (iii) pretreated with anti-mouse immunoglobulin or anti-θ serum plus complement.Pretreatment of macrophage monolayers with antibody plus complement, followed by a measurement of their 14C-uridine uptake could be used to test for anti-macrophage activity, for instance in certain anti-B cell or anti-T cell sera, or to detect cell surface antigens on macrophages. With this technique we demonstrated the presence of H-2 and Ia alloantigens on peritoneal macrophages. The presence of Ia antigens on macrophages may be of particular biological significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1975.tb00548.x
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  • 6
    Electronic Resource
    Electronic Resource
    CILIARY INHIBITION AND AXONEMAL MICROTUBULE ALTERATIONS IN FRESHWATER MUSSELS (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 253 (1975), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1975.tb19200.x
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in naturally aged Pinus pinea seeds (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 62 (1984), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The decline of germination observed after storage of Pinus pinea L. seeds up to 35 years at room temperature was accompanied by an increase in their ability to leak reducing sugars, probably as a consequence of membrane alterations due to senescence. Results of studies of the cell ultrastructure of viable and non-viable seeds before and after imbibition indicate that such is the case. Noticeable changes occurred in the ultrastructure of endosperm and embryo cells with germinability loss, even in the unimbibed condition. The main alterations were spherosome coalescence, plasmalemma separation from the cell wall and chromatin aggregation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb02803.x
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  • 8
    Electronic Resource
    Electronic Resource
    Extraction of tracheal sap from Citrus and analysis of its nitrogenous compounds (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 50 (1980), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Tracheal sap was extracted from sections of stems (0.5 to 1.5 cm in diameter and 7.5 to 15.0 cm in length) of orange trees (Citrus sinensis (L.) Osbeck cv. Washington Navel) by using a combination of the vacuum and liquid displacement methods. The volume of sap obtained and its concentration of nitrogenous compounds were dependent on the volume of displacing liquid used for the extraction. Four ml of water-saturated 1-butanol extracted essentially all of the xylem fluid present in the stem sections without apparent production of artifacts.The time of sampling affected the nitrogen concentration of the tracheal sap, but not the content of xylem nitrogen per volume of stem material. The orientation of the stems in the tree and the diameter of the stems had an effect on their contents of xylem nitrogen, with southeastern orientation and thinner stems showing higher concentrations.We could not detect the presence of ammonium, nitrites or proteins in the tracheal sap of orange trees. Most of the nitrogen was present as amino acids and about 2% of the total in the form of nitrates. The qualitative composition of amino acids, as determined by TLC, was the same both in winter and spring tracheal sap. The main components of the sap were proline and arginine in winter, and these amino acids together with asparagine and aspartic acid in spring.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1980.tb04466.x
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  • 9
    Electronic Resource
    Electronic Resource
    Surface Charge of Trypanosoma cruzi. Binding of Cationized Ferritin and Measurement of Cellular Electrophoretic Mobility (1977)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 24 (1977), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS The surface charge of epimastigote and trypomastigote forms of Trypanosoma cruzi was evaluated by means of binding of cationized ferritin to the cell surface as visualized by electron microscopy, and by direct measurements of the cellular microelectrophoretic mobility (EPM). Epimastigote forms had a mean EPM of -0.52 μm.s-1.V-1.cm and were lightly labeled with cationized ferritin. In contrast, bloodstream trypomastigotes had a much higher EPM (-1.14), and the surface was heavily labeled with cationized ferritin. When trypomastigotes from stationary phase cultures were isolated on DEAE cellulose columns, the mean EPM was found to be significantly lower (-0.63), and labeling with cationized ferritin decreased. With a mixed population containing epimastigote, trypomastigote, and intermediate forms, EPM values ranging between -0.70 to -1.14 were found. From these observations we conclude that there is a definite increase in negative surface charge during development from epi- to trypomastigote forms of T. cruzi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1977.tb04762.x
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  • 10
    Electronic Resource
    Electronic Resource
    Seasonal variation of nitrogenous compounds in the xylem sap of Citrus (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 59 (1983), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Samples of tracheal sap of Citrus sinensis (L.) Osbeck cv. Washington Navel were taken from field trees throughout the year and the nitrogen composition of the sap was determined. The nitrogenous fraction of the sap was composed mainly of free amino acids (92–97% of total nitrogen) and nitrates throughout the year. Proline was the most abundant amino acid during almost the entire cycle, and its concentration was especially high during the autumn and winter period. Nevertheless, a significant part (40–60%) of the total organic nitrogen was transported as arginine. Total nitrogen as well as amino acids and nitrates were maximal at spring flush. At spring flush and summer flush there was also a diversification of α-amino nitrogen among different amino acids. During the spring flush, nitrates, asparagine and γ-aminobutyric acid in the xylem sap seemed to have a radicular origin, whereas glutamic acid and arginine were released from the surrounding parenchyma. The results suggest a metabolic transformation in the wood parenchyma of nitrogenous compounds coming from the roots (including reduction of nitrates) and a turnover of different nitrogen metabolites between the xylem and surrounding cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1983.tb06298.x
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