Publication Date:
1996-07-12
Description:
The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP.RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kimura, K -- Ito, M -- Amano, M -- Chihara, K -- Fukata, Y -- Nakafuku, M -- Yamamori, B -- Feng, J -- Nakano, T -- Okawa, K -- Iwamatsu, A -- Kaibuchi, K -- New York, N.Y. -- Science. 1996 Jul 12;273(5272):245-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-01, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8662509" target="_blank"〉PubMed〈/a〉
Keywords:
3T3 Cells
;
Actins/metabolism
;
Amino Acid Sequence
;
Animals
;
Cattle
;
GTP Phosphohydrolases/*metabolism
;
GTP-Binding Proteins/*metabolism
;
Intracellular Signaling Peptides and Proteins
;
Isopropyl Thiogalactoside/pharmacology
;
Mice
;
Molecular Sequence Data
;
Muscle Contraction
;
Muscle, Smooth/physiology
;
Myosin Light Chains/metabolism
;
Myosin-Light-Chain Phosphatase
;
Oxazoles/pharmacology
;
Phosphoprotein Phosphatases/*antagonists & inhibitors/metabolism
;
Phosphorylation
;
Protein-Serine-Threonine Kinases/*metabolism
;
rho-Associated Kinases
;
rhoA GTP-Binding Protein
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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