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  • Articles  (5)
  • Acetylene reduction  (5)
  • 1985-1989
  • 1980-1984  (5)
  • 1970-1974
  • 1984  (3)
  • 1981  (2)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (5)
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  • Articles  (5)
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Keywords
Publisher
Years
  • 1985-1989
  • 1980-1984  (5)
  • 1970-1974
Year
Topic
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Utilisation du15N2 pour estimer la fixation d'azote et sa repartition chez les legumineuses (1981)
    Springer
    Plant and soil 63 (1981), S. 131-139 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Fixed-N distribution in plants ; 15N2 reduction ; Phaseolus vulgaris ; Trifolium arvense ; Trifolium badium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary A method for use of15N2 in the study of dinitrogen fixation by complex legumes-Rhizobium associations grown on soil is proposed. The procedure consists in labelling the soil atmosphere with15N2 during short periods of time, measuring15N enrichment in the plants and calculating the total nitrogen fixed during this period. The first results obtained with bean and clover plants demonstrate that after only 7 h of incubation, significative amounts of15N are measured in the plants to allow precise determination of C2H4/N2 ratio which ranged between 2.6 and 3.1 in this experiment. Over longer periods of time, such amounts are meaningfull to follow the pattern of N dynamic from the nodules to the reproductive organs of bean plants.
    Notes: Resumé Les auteurs proposent une méthode d'utilisation du15N2 pour l'étude de la fixation d'azote dans les associations complexes légumineuses-Rhizobium cultivées sur sol. La procédure consiste, à marquer l'atmosphère du sol à l'aide de15N2 et à calculer la quantité d'azote total fixé pendant cette période. Les premiers résultats obtenus sur des haricots et des trèfles démontrent qu'après seulement 7 h d'incubation, des quantités significatives de15N sont mesurées dans les plantes permettant ainsi de déterminer précisément la valeur du rapport C2H4/N2 qui a été établi entre 2,6 et 3,1 dans les conditions de l'expérience. Sur de longues périodes, ces mêmes quantités se sont avérées suffisantes pour suivre la dynamique de l'azote des nodules vers les organes reproducteurs de plants de haricots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02374592
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of lime additions to acid strip-mine spoil on survival, growth and nitrogen fixation (acetylene reduction) of several woody legume and actinomycete-nodulated species (1984)
    Springer
    Plant and soil 79 (1984), S. 353-367 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Actinomycete-nodulated ; Legume ; Lime ; Mine spoil ; Nitrogen fixation ; Reclamation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An acid mine spoil in Southern Indiana was amended with lime (CaCO3) (0.0, 12.5, 25 and 39t/ha) and planted withElaegnus umbellata Thunb.,Alnus glutinosa Gaertn.,Robinia pseudoacacia L.,Robinia fertilis Ashe, ‘Arnot’,Myrica pensylvania Lois,Caragana arborescens L. andShepherdia argentea Nutt. Survival and soil data were collected periodically and plants were harvested 15 months after planting. Nodule and top dry weights were determined and acetylene reduction assays performed on the nodules. Addition of lime caused significant increases in pH, and 39 t/ha of lime were required to maintain a pH above 5.5. Survival of plant material was greatest at the highest lime addition, although response of individual species varied.Elaeagnus umbellata, R. pseudoacacia, R. fertilis ‘Arnot’, andA. glutinosa appeared more tolerant of the harsh conditions. OnlyC. arborescens showed a linear increase in top dry weight due to lime addition.Alnus glutinosa andS. argentea achieved statistically the same growth regardless of pH, andR. fertilis ‘Arnot’ andE. umbellata did not increase in top dry weight above an addition of 25 t/ha.Robinia pseudoacacia achieved maximum top dry weight at 25 t/ha, whereasM. pensylvanica growth declined with increasing pH. Nodule dry weights increased with increasing pH; however,S. argentea showed greater nodule dry weights at lower lime levels. Acetylene reduction rates increased with lime addition.Elaegnus umbellata did not respond above 25 t/ha lime, whereasA. glutinosa did not show an increase until this point.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02184328
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  • 3
    Electronic Resource
    Electronic Resource
    A comparison between the acetylene reduction method, the isotope dilution method and the total nitrogen difference method for measuring nitrogen fixation in lucerne (Medicago sativa L.) (1984)
    Springer
    Plant and soil 81 (1984), S. 177-184 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Lucerne ; 15N Nitrogen fixation ; Rhizobium meliloti
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In view of the increasing need for the exact estimation of the input of nitrogen in agroecosystems, an application of the acetylene-reduction technique was developed. The technique, consisting of a plastic bag incubation system, using propane as an internal standard of the apparent volume, made it possible to carry out repeated incubations on the same plant system. The evaluated technique included studies of the diffusion of ethylene and propane in a soil column, as well as studies of the optimal substrate concentration and grade of purity of the substrate. In addition, the conversion factor between amounts of reduced acetylene as compared wtih reduced dinitrogen was determined by15N2 incubations to be 4.41. The developedin situ acetylene-reduction technique was compared with an isotope dilution method and a total nitrogen difference method. By comparing the derived total nitrogen fixation values from each with the value derived from the acetylene-reduction method; it was shown that the values differed significantly. The acetylene-reduction method gave the highest nitrogen fixation values, the isotope dilution the lowest values and the total nitrogen difference method was intermediate. No statistical significant difference existed between the two different reference crops used in the isotope dilution method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02197149
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  • 4
    Electronic Resource
    Electronic Resource
    15N2 incorporation and acetylene reduction by Azospirillum isolated from rice roots and soils (1981)
    Springer
    Plant and soil 61 (1981), S. 429-436 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Azospirillum ; 15N2 incorporation ; Rice cultivar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nitrogen fixation by strains of Azospirillum isolated from several rice soils and rice cultivars was investigated by15N2 incorporation and C2H2 reduction. C2H2 reducing ability markedly varied among the strains obtained from soils differing widely in their physico-chemical properties. Large variations in15N2 incorporation by Azospirillum isolated from the roots of several rice cultivars were also noticed. The present study reveals that rice cultivars harbour Azospirillum with differential N2-fixing ability and that plant genotype is of importance for optimal associations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02182023
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  • 5
    Electronic Resource
    Electronic Resource
    A multiple-trait breeding program for improving the symbiosis for N2 fixation betweenMedicago sativa L. andRhizobium meliloti (1984)
    Springer
    Plant and soil 82 (1984), S. 303-314 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Disease resistance ; Host-Rhizobium strain interaction ; Nitrate reductase ; Nodule enzymes ; Nodule mass ; 15N evaluation ; Plant vigor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The goal of breeding alfalfa for increased N2 fixation potential is addressed. A chronological progression of breeding, physiological, microbiological, and plant pathological research is described. Studies describing the interrelationships among plant morphological, plant physiological, andRhizobium effectiveness traits are summarized. It was concluded that N2 fixation in alfalfa is affected by coordinated responses among many physiological and biochemical traits. The simultaneous improvement of many factors in the symbiosis requires a comprehensive multiple-step breeding program. The current program includes selection in the glasshouse for seedling vigor,Rhizobium preference, shoot growth, nodule mass, root growth, nitrogenase (as measured by acetylene reduction), and nodule enzyme activity. The inclusion of additional selection traits is anticipated. Field evaluations of N2 fixation potential of alfalfa populations are made with15N isotope dilution techniques. Plant germplasm sources used in the breeding program include several heterogeneous populations which have good combining ability and pest resistance when they are intercrossed. Significant progress has been made in achieving the goal of breeding alfalfa for improved N2 fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02184269
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