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  • ASTROPHYSICS  (233)
  • Life and Medical Sciences  (137)
  • Lunar and Planetary Science and Exploration
  • 1995-1999
  • 1980-1984  (370)
  • 1960-1964
  • 1983  (197)
  • 1981  (173)
Collection
Keywords
Publisher
Years
  • 1995-1999
  • 1980-1984  (370)
  • 1960-1964
Year
  • 1
    Electronic Resource
    Electronic Resource
    Effects of millimeter-wave radiation on monolayer cell cultures. II. Scanning and transmission electron microscopy (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 141-150 
    Details
    ISSN: 0197-8462
    Keywords: millimeter-wave radiation ; BHK-21/C13 cells in monolayer culture ; scanning electron microscopy ; transmission electron microscopy ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Both thermal and athermal effects of millimeter-wave radiation on BHK-21/C13 cells were sought using scanning and transmission electron microscopy in conjunction with an in vitro technique that allows direct exposure of monolayer cultures to high average power densities. Culture dishes were irradiated by placing them on the open end of an E- or U-band wave guide. This technique exposes different regions of the cell monolayer lying along the longer axis of the wave guide aperture to varying power densities ranging from zero at each edge to twice the average power density at the center.Cell ultrastructure was unaffected by microwave radiation for 1 hour (41.8 or 74.0 GHz, average power densitites = 320 or 450 mW/cm2, respectively) with or without cooling by rapid recirculation of the culture medium. Temperature in recirculated cultures was held at 37.2 °C, and that in noncooled cultures never exceeded 42 °C during irradiation at either power density. In contrast, cell morphology was affected by microwave exposure whenever irradiation conditions were altered so that the temperature of the monolayer reached or exceeded 44.5 °C. Ultrastructural alterations included breakage of cell processes, progressive detachment of cells from the substrate, increased clumping of heterochromatin in the nuclei, and the appearance of large empty vesicles in the cytoplasm. Such morphological changes resulted from either application of higher average power densities or irradiation at the power densities described above at a higher ambient temperature (〉38.5°C).
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250020205
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of millimeter-wave radiation on monolayer cell cultures. I. Design and validation of a novel exposure system (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 123-140 
    Details
    ISSN: 0197-8462
    Keywords: millimeter-wave radiation ; BHK-21/C13 cells in monolayer culture ; quantitative autoradiography ; ribonucleic acid (RNA) synthesis ; protein synthesis ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A method has been devised whereby both the thermal and possible athermal biological effects resulting from microwave radiation can be assessed. Monolayer cultures of BHK-21/C13 cells were grown on microwave-transparent polystyrene coverslips, placed directly on the open end of a wave guide, and irradiated for 1 hour. In experiments seeking athermal biological effects of millimeter waves, culture medium was continuously recirculated over the cells to prevent temperature increases greater than 0.1 °C. Incorporation of 3H-uridine into RNA and of 3H-methionine into protein was quantified by measurement of optical densities of the autoradiographs in contiguous rectangular regions corresponding to portions of the cell monolayer immediately above the wave guide aperture and lying along its longer axis. Since power density was shown to vary with position along this axis according to a cosine2 relationship, it was possible to assess the extent of microwave effects on macromolecular synthesis at power densities ranging from zero at each edge to twice the average power density at the center of the waveguide.Monolayer cultures maintained at 37.2 °C by recirculation of the medium did not show microwave-induced changes in synthesis of RNA and protein (41.8 or 74.0 GHz at average power densities of 320 or 450 mW/cm2, respectively). Since macromolecular synthesis was examined both during and after irradiation, our results exclude both transient and persistent athermal biological effects of acute exposure to millimeter waves. In contrast, irradiation of cultures incubated in a small volume of nonrecirculated medium resulted in 1) marked heating of the monolayer, 2) a graded decline in macromolecular synthesis with increasing incident power, and 3), in some cases, destruction of the cell monolayer in the region immediately above the center of the waveguide aperture.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250020204
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of millimeter-wave radiation on monolayer cell cultures. III. A search for frequency-specific athermal biological effects on protein synthesis (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 2 (1981), S. 151-159 
    Details
    ISSN: 0197-8462
    Keywords: protein synthesis ; quantitative autoradiography ; BHK-21/C13 cells ; millimeter-wave radiation ; frequency-specific biological effects ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A method recently developed in this laboratory has been used to directly expose BHK-21/C13 cells to high levels of microwave radiation without significant microwave-induced heating (≤ 0.1 °C). Monolayer cultures were grown on microwave-transparent polystyrene coverslips, placed on the open end of a wave guide, and maintained at 37.2 °C during irradiation at frequencies in both the E- and U-bands (average power densities 292 and 177 mW/cm2, respectively). Effects of microwave radiation were assessed at 0.1 GHz increments in the ranges of 38-48 GHz and 65-75 GHz. Protein synthesis was measured in quadruplicate cultures that were allowed to incorporate labeled methionine during the 15-minute period of microwave irradiation. Autoradiographs of each monolayer culture were scanned along the region corresponding to the longer axis of the wave guide aperture using a microdensitometer to quantify incorporation. Since microwave power incident on the cells was previously shown to vary along this axis according to a cosine2 relationship from zero at each edge of the wave guide to twice the average power density at the center of the wave guide, this technique should reveal biological effects that might only be manifested in narrow amplitude domains or “power windows.” Observations of protein synthesis in monolayer cultures irradiated at 202 closely spaced frequencies in the E- and U-bands failed to reveal changes associated with microwave exposure. Thus no evidence was obtained in support of the existence of frequency-specific athermal biological effects of microwaves. In addition, no support was found for the existence of amplitude-specific “power windows”.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250020206
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  • 4
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    The filaments of NGC 1275 - A collision between a galaxy and an accretion flow? (1983)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: Morphological evidence is presented (in the form of 3-A-bandpass Fabry-Perot images made with a CCD camera) that the two bright emission-line systems seen toward the galaxy NGC 1275 arise from a high-velocity impact of a foreground galaxy upon the accretion flow of gas cooling in the center of the Perseus cluster. The uniquely high optical-line luminosity of NGC 1275, in comparison with other central galaxies in clusters observed to have cooling flows, may be explained by energy deposited during the collision. Using additional information from 21-cm and extinction measurements, a rough model of the interaction is developed. Problems remain with this model - such as the likelihood of a gas-rich system penetrating to the cluster center. The kinematic structure of the optically emitting gas shows additional complex structure near the nucleus of NGC 1275.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 2 - Letters to the Editor (ISSN 0004-637X); 275; L27-L31
    Format: text
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    NASA TECHNICAL REPORTS
  • 5
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    Coordinated Einstein and IUE observations of a disparitions brusques type flare event and quiescent emission from Proxima Centauri (1983)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: The Einstein Imaging Particle Counter observed a major X-ray flare in its entirety during a 5-hr period of simultaneous observations, with the IUE, of the dM5e flare star Proxima Centauri in August, 1980. The detailed X-ray light curve, temperature determinations during various intervals, and UV line fluxes obtained before, during, and after the flare indirectly indicate a 'two-ribbon flare' prominence eruption. The calculated ratio of coronal to bolometric luminosity for the event is about 100 times the solar ratio. The Proxima Cen corona is analyzed in the context of static loop models, in light of which it is concluded that less than 6% of the stellar surface seems to be covered by X-ray emitting active regions.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal; vol. 267
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    Rapid variability of 10-140 keV X-rays from Cygnus X-1 (1981)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: On five occasions in 1977 and 1978, Cygnus X-1 was observed using the low-energy detectors of the UCSD/MIT Hard X-ray and Low-Energy Gamma Ray experiment on the HEAO 1 satellite. Rapid (times between 0.08 and 1000 sec) variability was found in the 10-140 keV band. The power spectrum was white for frequencies between 0.001 and 0.05 Hz and was proportional to the inverse of the frequency for frequencies between 0.05 and 3 Hz, indicating correlations on all time scales less than approximately 20 s. The shape of the energy spectrum was correlated with intensity; it was harder at higher intensity. If the emission is produced by Comptonization of a soft photon flux in a hot cloud, the heating of the cloud cannot be constant; it must vary on time scales up to approximately 20 s. A variable accretion rate could cause the observed effects.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal; vol. 246
    Format: text
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    NASA TECHNICAL REPORTS
  • 7
    Electronic Resource
    Electronic Resource
    Relative humidity affects the intercellular spaces and cell contacts of the kidney epithelium of the terrestrial snail Helix aspersa müller (1983)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 178 (1983), S. 313-322 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of relative humidity on hemolymph osmolarity and on kidney ultrastructure are explored in Helix aspersa. The snails are active at 95% relative humidity and less active at 50% relative humidity. The hemolymph osmotic pressure increases with the decrease of relative humidity. Pericardial fluid and hemolymph collected from the heart contain similar amounts of total proteins, and both fluids display hemocyanin molecules in negatively stained preparations. When the snails are kept in an atmosphere of 95% relative humidity, numerous wide intercellular spaces are observed in the single-layered-kidney epithelium. The spaces are almost absent when the snails are kept at 50% relative humidity. It is suggested that prourine is formed through a paracellular junctional pathway across the single-layered kidney epithelium, and that the pericardial cavity is not the site of prourine formation. The septate junctions joining the kidney epithelial cells form a continuous belt of intimate contact in the paracellular pathway of prourine. Long septate junctions with many septa are present in the kidneys of snails from the atmosphere of 50% relative humidity, whereas short septate junctions with fewer septa are found in the kidneys of snails from the atmosphere of 95% relative humidity. It is possible that the longer septate junctions with many septa reduce prourine formation across the kidney sac epithelium.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051780308
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  • 8
    Electronic Resource
    Electronic Resource
    Rotaviruses code for two types of glycoprotein precursors (1983)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 22 (1983), S. 151-160 
    Details
    ISSN: 0730-2312
    Keywords: dog pancreatic microsomes ; signal sequences ; rotavirus glycoproteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Rotaviruses are nonenveloped viruses that code for two glycoproteins: a structural glycoprotein (VP7) and a nonstructural glycoprotein (NS29). The precursor to VP7 (37K) was shown to contain a 1.5K cleavable signal sequence. The 37K precursor was authentically processed (signal sequence cleaved and the polypeptide “core” glycosylated) when synthesized in a cell-free system supplemented with dog pancreatic microsomes. Similar experiments were performed with the nonstructural glycoprotein precursor (20K); however, the 20K precursor contained an integral (noncleavable) signal sequence. Both precursors were inserted into membranes cotranslationally and both glycosylated products underwent post-translational oligosaccharide processing. The results suggest a morphogenetic scheme for the simian rotavirus SA11.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240220304
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  • 9
    Electronic Resource
    Electronic Resource
    Radioiodination studies of the envelopes from Xenopus laevis eggs (1983)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 22 (1983), S. 235-244 
    Details
    ISSN: 0730-2312
    Keywords: fertilization ; egg envelopes ; glycoproteins ; molecular topography ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: To investigate the molecular basis of the observed morphological and biological characteristics of coelomic egg envelopes (CE), vitelline envelopes (VE), and fertilization envelopes (FE) of Xenopus laevis eggs, envelopes were radioiodinated under a variety of conditions: in situ, isolated and intact, or solubilized. The distribution of 125I in envelope components was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Each envelope type displayed unique profiles when iodinated in the intact state. A major constituent of VE, the 41,500 molecular weight component, was not labeled in the intact state, although the corresponding component of CE was heavily labeled. After dissociation of the envelope by guanidine-HCl or sodium dodecyl sulfate, all of the components could be radioiodinated. However, when the envelopes (VE and FE) were dissolved by heating and subsequently radioiodinated by lactoperoxidase, the resulting radioactivity profile was similar to that of the intact envelopes, suggesting that in the heat-dissolved envelope, the individual components retain similar structural relations as in the intact envelope. Quantitative but not qualitative differences were found between the inner and outer aspects of VE and FE. The significance of these findings is discussed in relation to what is known about the morphological, biological, and molecular properties of the envelopes.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240220405
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  • 10
    Electronic Resource
    Electronic Resource
    Regulation of granulopoiesis and distribution of granulocytes in early phase of bacterial infection (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 109 (1981), S. 17-24 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Studies have been carried out to determine the effect of bacterial infection on CSF production, CFU-C activation, and bacterial clearance by mature granulocytes in mice infected with Escherichia coli. These studies have shown that immediately after bacterial infection (5 minutes), serum colony-stimulating factor (CSF) levels and bone marrow colony-forming units in culture (CFU-C) levels are elevated. This is followed by oscillator rises in both of these parameters and the appearance of granulocytes in the infected site. With clearance of bacteria, CSF and CFU-C levels return to normal. These studies have indicated further that bacterial infection is a major stimulus for granulocyte production through the CSF-CFU-C system and that clearance of bacteria by mature granulocytes may serve as a negative feedback regulatory arm.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041090103
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