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  • spermatozoa  (5)
  • Wiley-Blackwell  (5)
  • American Chemical Society
  • American Geophysical Union
  • 2015-2019
  • 1980-1984  (5)
  • 1950-1954
  • 1982  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Effect of albumin on fertilization of mouse ova in vitro (1982)
    New York, NY : Wiley-Blackwell
    Gamete Research 6 (1982), S. 305-313 
    Details
    ISSN: 0148-7280
    Keywords: albumin ; capacitation ; fertilization ; spermatozoa ; zona pellucida ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Serum albumin is an obligatory component of the incubation medium for the fertilization of mouse ova. Normal, untreated bovine serum albumin supports high rates of fertilization of cumulus-free ova both with and without their zonae pellucidae. Heat-treated or trichloroacetic acid-extracted bovine serum albumin is unable to support the fertilization of a majority of zona-intact ova but fertilization of zona-free ova is unimpaired. Spermatozoa incubated in medium containing heated bovine serum albumin fertilize zona-intact ova when 2 mM caffeine is present but the progress of sperm head decondensation is delayed when compared to normal controls. Trichloroacetic acid extracted BSA preferentially and irreversibly inhibits zona penetration by spermatozoa, but this effect is not mediated by an inhibition of spermatozoal motility or zona-binding ability. This effect occurs after only a 10-min preincubation of the spermatozoa in the extracted BSA or when the medium contains only a 10% (v/v) proportion of this albumin. It is estimated that mouse spermatozoa under the conditions used take 2 hr to penetrate the zonae pellucidae of 50% of ova and effect fertilization.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120060403
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  • 2
    Electronic Resource
    Electronic Resource
    Fertilization of cumulus-free, zona-intact mouse ova in vitro at high and low sperm concentrations (1982)
    New York, NY : Wiley-Blackwell
    Gamete Research 5 (1982), S. 61-70 
    Details
    ISSN: 0148-7280
    Keywords: mouse ; spermatozoa ; concentration ; fertilization ; ova ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effect of varying the sperm concentration between 2 × 105 sperm/ml and 8 × 106 sperm/ml on fertilization of cumulus-free, zona-intact F1 (CBA × C57BL) mouse ova by QS and F1 (CBA × C57BL) mouse spermatozoa was studied. The spermatozoa from both strains of mice exhibited optimal fertilization rates at 2 × 106 sperm/ml. However, at sperm concentrations greater than 4 × 106 sperm/ml and less than 1 × 106 sperm/ml, fertilization rates were significantly reduced. F1 spermatozoa were more susceptible to dilution than QS spermatozoa. A significant interaction between strain and sperm concentration indicated that the two strains produced different fertilization rates at different sperm densities.Extracts of epididymal fluid, medium from capacitated spermatozoa, or ampulla fluid did not improve the fertilization rate at 2 × 105 sperm/ml, but retaining the cumulus oophorus did. The decrease in fertilization rate at 8 × 106 sperm/ml can in part be attributed to a nondialysable inhibitor from the neat sperm preparation that appeared to be of epididymal origin.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120050107
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  • 3
    Electronic Resource
    Electronic Resource
    The structure and formation of rolled and crested bull spermatozoa (1982)
    New York, NY : Wiley-Blackwell
    Gamete Research 5 (1982), S. 263-269 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; bull ; rolled sperm ; crested sperm ; knobbed sperm ; testis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The ejaculate of a Friesian bull contained three abnormalities: rolled, crested, and knobbed spermatozoa. The rolled form had marked lateral curvature of the head, occasionally forming a complete tube. All three forms were of testicular origin and it is likely that the first two were developmentally related.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120050306
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  • 4
    Electronic Resource
    Electronic Resource
    Use of zona-free hamster ova to assess sperm fertilizing ability of bull and stallion (1982)
    New York, NY : Wiley-Blackwell
    Gamete Research 5 (1982), S. 217-227 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; bull ; stallion ; vitelli ; hamster ; fertilization ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Zona-free hamster ova interacted with bull and stallion spermatozoa after treatment of ejaculated semen to capacitate the sperm cells. Sperm conditioning by prolonged incubation in BWW medium (18-26 hr) prior to insemination was effective for capacitation of bull and stallion sperm. Preincubation of bull sperm for 70 or 105 min in defined medium (DM) with NaCl content elevated to result in 350 m0sM/kg also led to penetration of hamster vitelli. More rapid sperm conditioning was possible and higher proportions of interacting vitelli followed insemination with bull or stallion sperm exposed to high ionic strength DM (380-390 m0sM/kg) for 10 min before incubation in isotonic DM prior to insemination, the treatment adopted in subsequent work.Initial efforts to assess relative fertilizing ability of freshly ejaculated semen from two fertile bulls (A and B) in A1 usage resulted in uniformly high ( 〉 90%) levels of sperm-vitelli interaction (for both) when the hamster ova employed resulted from superovulation with PMSG and HCG. Following use of ova from untreated hamsters sperm samples of bull A and bull B interacted with 53.8% and 84.9% (P 〈 0.05) of zona-free hamster ova, respectively. Conception data (60-90 day nonreturn rates) resulting from A1 with semen collected during the same interval but processed and stored in liquid nitrogen prior to use revealed an inverse relationship to proportions of vitelli interacting with fresh sperm; nonreturn rates were 69.3% and 66.3% for bull A and bull B, respectively. A similar treatment effected capacitation of frozen-stored bull semen to enable sperm-vitelli interaction. These findings encourage additional efforts to correlate testing of processed semen with fertility.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120050302
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  • 5
    Electronic Resource
    Electronic Resource
    Circular DNA in human and boar spermatozoa (1982)
    New York, NY : Wiley-Blackwell
    Gamete Research 5 (1982), S. 379-393 
    Details
    ISSN: 0148-7280
    Keywords: circular DNA ; spermatozoa ; human ; boar ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Circular DNA molecules were isolated from human and boar whole spermatozoa or spermatozoal nuclei and measured for size by electron microscopy. The DNA molecules derived from both mammals were heterogeneous in size ranging from 0.07 to 17 μm; nearly 75% of the molecules were ≤0.5 μm in length. The mean lengths were 1.0 μm and 1.5 μm for circular DNAs isolated from human and boar spermatozoa, respectively. The origin and function of these molecules remains unknown.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120050408
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