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  • Gibberellin  (5)
  • Springer  (5)
  • American Association for the Advancement of Science
  • American Association of Petroleum Geologists (AAPG)
  • American Chemical Society (ACS)
  • International Union of Crystallography
  • 1980-1984  (5)
  • 1960-1964
  • 1980  (5)
Collection
Publisher
  • Springer  (5)
  • American Association for the Advancement of Science
  • American Association of Petroleum Geologists (AAPG)
  • American Chemical Society (ACS)
  • International Union of Crystallography
Years
  • 1980-1984  (5)
  • 1960-1964
Year
  • 1
    ISSN: 1432-2048
    Keywords: Gibberellin ; Growth temperature ; Lactuca ; Temperature (gibberellin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between elongation growth and the incorporation of [3H]gibberellin A1 ([3H]GA1) into a 2,000g pelletable (2KP) fraction from lettuce (Lactuca sativa L., cv. Arctic) hypocotyl sections has been examined. Sections were loaded with incremental amounts of GA1 under conditions where growth was arrested (5° C) or permitted (30° C) and, after 16 h, all were transferred to a GA-free medium at 30° C. Growth and 2KP radioactivity were measured at this point and after a further 24 h in the chase medium. Uptake was reduced by 80% at 5° C, as compared to 30° C, but 2KP labelling and protein synthesis were only reduced by half. The growth rate of the 5° C pretreated sections during the chase period was comparable to that observed during the pulse in the 30° C material but the dose/response relationship was flatter. Low temperature sections incorporated a much higher percentage of GA1 uptake into the 2KP fraction (27% at maximum) but the absolute levels of labelling at this temperature were lower than those measured at 30° C. The data are interpreted as showing that 2KP labelling is not a consequence of growth. It must either precede response or be an unconnected concurrent process.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Planta 147 (1980), S. 444-450 
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Auxin ; Cytokinin ; Decapitation ; Fruit-set ; Gibberellin ; Parthenocarpy ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of removing the apical shoot and different leaves above and below the flower on the fruit-set of unpollinated pea ovaries (Pisum sativum L. cv. Alaska) has been studied. Unpollinated ovaries were induced to set and develop either by topping or by removing certain developing leaves of the shoot. Topping had a maximum effect when carried out before or on the day of anthesis, and up to four consecutive ovaries were induced to set in the same plant. The inhibition of fruit-set was due to the developing leaves and not to the apex. The third leaf above the first flower, which had a simultaneous development to the ovary, had the stronger inhibitory effect on parthenocarpic fruit-set. The application of different plant-growth regulators (indoleacetic acid, naphthylacetic acid, 2,4-dichlorophenoxyacetic acid, gibberellic acid, benzyladenine and abscisic acid) did not mimic the negative effect of the shoot.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Planta 147 (1980), S. 451-456 
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Auxin ; Fruit-set ; Gibberellin ; Parthenocarpy ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of parthenocarpic fruits of Pisum sativum L. cv. Alaska was induced by the application of different plant-growth regulators in aqueous solution to the emasculated ovaries in untopped plants. At least one compound in each of the groups of auxins (2,4-dichlorophenoxyacetic acid), cytokinins (benzyladenine), and gibberellins (gibberellic acid) was found active. Gibberellic acid (GA3), however, was the only substance which produced pods similar to those of fruits with seeds. The length of the pods obtained by GA3 was a linear function of the logarithm of the concentration of GA3 in the solution. The effect of GA3 (at a concentration which produced 50% of the maximum pod length) was enhanced by a simultaneous application of 2,4-dichlorophenoxyacetic acid. Abscisic acid (ABA) counteracted the effect of GA3 and of topping. The results suggest that gibberellins and ABA may exert a major regulatory control in natural fruit-set. Peas can be used for the assay of fructigenic activity and is an advantageous material for the study of the mode of action of gibberellins on fruit-set.
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  • 4
    ISSN: 1432-2048
    Keywords: Gibberellin ; Growth temperature ; Lactuca ; Temperature (gibberellin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between elongation growth and the incorporation of [3H]gibberellin A1 ([3H]GA1) into a 2,000g pelletable (2KP) fraction from lettuce (Lactuca sativa L., cv. Arctic) hypocotyl sections has been examined. Sections were loaded with incremental amounts of GA1 under conditions where growth was arrested (5° C) or permitted (30° C) and, after 16 h, all were transferred to a GA-free medium at 30° C. Growth and 2KP radioactivity were measured at this point and after a further 24 h in the chase medium. Uptake was reduced by 80% at 5° C, as compared to 30° C, but 2KP labelling and protein synthesis were only reduced by half. The growth rate of the 5° C pretreated sections during the chase period was comparable to that observed during the pulse in the 30° C material but the dose/response relationship was flatter. Low temperature sections incorporated a much higher percentage of GA1 uptake into the 2KP fraction (27% at maximum) but the absolute levels of labelling at this temperature were lower than those measured at 30° C. The data are interpreted as showing that 2KP labelling is not a consequence of growth. It must either precede response or be an unconnected concurrent process.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 150 (1980), S. 70-81 
    ISSN: 1432-2048
    Keywords: Aleurone ; α-Amylase ; Endoplasmic reticulum ; Gibberellin ; Hordeum ; Organelle isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in the level of the endoplasmicreticulum (ER) marker enzyme cytochrome-c reductase (EC 1.6.2.1) were followed with time of imbibition of de-embryonated half-seeds of barley (Hordeum vulgare L.) and the subsequent incubation of their aleurone layers in gibberellic acid (GA3) and H2O. During imbibition there is an increase in the level of cytochrome-c-reductase activity and in the amount of 280-nm absorbance associated with this enzyme. When aleurone layers are incubated for a further 42 h in water, there is a doubling of the cytochrome-c-reductase activity. In GA3, the activity of cytochrome-c reductase reaches a maximum at 24 h of incubation and thereafter falls to below 70% of its level at the beginning of the incubation period. Changes in the cytochrome-c-reductase activity correlate with changes in the fine structure of the aleurone cell. The ER isolated in low Mg2+ from aleurone layers incubated in buffer for up to 18 h has buoyant density of 1.13–1.14 g cc-1 while that from layers incubated in GA3 for 7.5–18 h has a density of 1.11–1.12 g cc-1. The α-amylase (EC3.2.1.1) isolated with the organelle fraction by Sepharose gel filtration is associated with the ER on isopycnic and rate-zonal density gradients, and its activity can be enhanced by Triton X-100. The soluble α-amylase fraction from Separose-4B columns, on the other hand, is not Triton-activated but is acid-labile. Acid phosphatase (EC3.1.3.2) is distributed in at least three peaks on isopycnic gradients. In low Mg2+ the second peak of activity has a density of 1.12 g cc-1 in GA3-treated tissue and 1.13–1.14 g cc-1 in H2O-treated tissue. With high-Mg2+ buffers, this peak of phosphatase activity disappears. Acid-phosphatase activity is not enhanced by Triton X-100 nor is it acid-labile.
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