ISSN:
1432-1424
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary The inactivation (desensitization) of the acetylcholine receptor by carbanylcholine, a stable analogue of acetylcholine, has been investigated in eel Ringer's solution, pH 7.0, 0°C, by measurements of (i) ion flux and (ii) the kinetics of the reaction of [125I]-α-bungarotoxin with the receptor. The effect of preincubation with carbamylcholine is significantly different in the two types of measurement. In both the receptor-controlled flux of inorganic ions and the toxin-binding kinetics a biphasic process has been observed (Hess, G.P., Lipkowitz, S., Struve, G.E., 1978,Proc. Nat. Acad. Sci. USA 75:1703; Hess, G.P. et al., 1975,Biochem. Biophys. Res. Commun. 64: 1018; Bulger, J.E. et al., 1977,Biochemistry 16: 684), only the initial fast phase of which is inhibited and the subsequent slow phase persists. However, preincubation with carbamylcholineper se has no effect on the toxin reaction. The results obtained are consistent with the proposal of Katz and Thesleff (Katz, B., Thesleff, S., 1957,J. Physiol. (London) 138: 65) that the active form of the receptor is converted to an inactive form in the presence of acetylcholine receptor ligands, and with our previous experiments (Hess et al., 1978) which indicated that one receptor form is responsible for the initial fast phase of both the receptor-controlled ion flux and the toxin binding reaction, and that its conversion to the other form results in the slow phases in these two measurements.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01875964
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