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  • 1
    Electronic Resource
    Electronic Resource
    Organization and unusual expression of histone genes in the sea starPisaster ochraceus (1987)
    Springer
    Journal of molecular evolution 25 (1987), S. 29-36 
    Details
    ISSN: 1432-1432
    Keywords: Histone genes ; Echinoderms ; Maternal mRNA ; Development ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization and maternal expression of histone genes differ greatly between the two echinoderm classes represented by the sea stars and sea urchins. We have isolated and characterized a 5.3-kb genomic DNA fragment encoding core histone genes in the sea starPisaster ochraceus. The majority of histone genes are organized as tandem repeats of the 5.3-kb fragment, which is present in as many as 700 copies per haploid genome. The identity, precise location, and transcriptional polarity of individual core histone genes within the repeat were determined by DNA sequence analyses. The gene order in the sea star (H2B, H2A, H4, H3) is different from that in sea urchins (H2B, H3, H2A, H4). What is remarkable is the low level of maternal histone mRNA inP. ochraceus eggs relative to that in sea urchins. This observation supports a recent suggestion that major changes in mode of gene expression, rather than in gene organization or copy number, can be correlated with major events in echinoderm evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02100038
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning and expression in Escherichia coli of a Thermoanaerobacter cellulolyticus gene coding for heat-stable β-glucanase (1987)
    Springer
    Applied microbiology and biotechnology 25 (1987), S. 480-483 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A 4.8 kb HindIII fragment of Thermoanaerobacter cellulolyticus DNA cloned in Escherichia coli was shown to direct the synthesis of β-glucanase. The enzyme produced by the transformant was extremely heat-stable and the optimum temperature for the enzyme reaction was 80°C. The cloned enzyme could hydrolyse carboxymethyl cellulose and lichenan, but could not digest laminarin, xylan and cellobiose. Although T. cellulolyticus secreted cellulase(s) into the medium, most of the cloned enzyme activity was detected only in cytoplasm in the recombinant clone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253323
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  • 3
    Electronic Resource
    Electronic Resource
    Tissue distribution of heavy metals and their variations with age, sex, and habitat in Japanese serows (Capricornis crispus) (1987)
    Springer
    Archives of environmental contamination and toxicology 16 (1987), S. 551-561 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract Baseline data on heavy metal accumulations (Fe, Mn, Zn, Cu, Co, Ni, Pb, Cd, and Hg) in organs and tissues, and their variations with age, sex, and habitat in free-ranging Japanese serows were determined. The metal concentrations were high in liver and kidney and low in brain and muscle. Also, high accumulations of Mn and Ni in gastrointestinal organs may be due to an undeveloped excretory system for these metals by bile. About 40% of the Hg burden in the whole body was in the fleece, indicating that a major portion of Hg body burden is excreted by moulting. The metal concentrations varied widely with metal species and also with growth stage or age, especially during the fetal, fawn and puberty periods. Metal burdens of the fetus were very low compared with those of their mothers, so parturition did not change the whole-body burdens/concentrations of metals in the mothers. Iron in the muscle and liver, and Cu in the liver showed significantly higher concentrations in the males than in the females. A significant difference of Cd concentrations was found between habitats of the serow.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01055811
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  • 4
    Electronic Resource
    Electronic Resource
    Heavy metal accumulations and their recent changes in southern minke whalesBalaenoptera acutorostrata (1987)
    Springer
    Archives of environmental contamination and toxicology 16 (1987), S. 209-216 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract The concentrations of heavy metals in the livers of southern minke whales,B. acutorostrata, were determined by atomic absorption spectrophotometry, and their accumulation levels and variations were compared to food habit, and biological parameters. The concentration levels and bioconcentration factors of metals in the minke whale were relatively high for Fe and Cd and low for Hg, when compared to seals and small-toothed cetaceans from different waters. For most of the metals examined, the concentrations were log-normally distributed. The concentrations of Fe, Cd, and Hg were positively correlated with age, but a correlation was not observed for the other metals. While the hepatic Fe concentration linearly increased with age, the concentrations of Cd and Hg increased up to the age of about 20 years and thereafter decreased year by year. Such unusual age trends of Cd and Hg are considered to be due to recent changes of the amount of food intake by the minke whale, as a result of structural disturbances in the Antarctic marine ecosystem due to commercial whaling. Also, the hepatic concentrations of metals varied between sexes, and with reproductive status of the matured female; the concentration of Fe was lower in the females than the males, and Fe, Pb, Ni, and Co concentrations in the matured females decreased with progress of gestation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01055801
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  • 5
    Electronic Resource
    Electronic Resource
    Multivalent regulation of the nusA operon of Escherichia coli (1987)
    Springer
    Molecular genetics and genomics 206 (1987), S. 185-191 
    Details
    ISSN: 1617-4623
    Keywords: nuA operon ; Regulation ; RNA polymerase ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The rate of synthesis and intracellular content of the NusA protein, a transcription termination factor, were determined for wild-type and nusA and/or nusB mutants of Escherichia coli. Both the rate and content of NusA in wild-type strains were similar to that of the RNA polymerase σ subunit, a transcription initiation factor, on a molar basis, and about 30%–40% the levels of RNA polymerase ββ′ subunits. At the stationary phase of cell growth, the values increased in parallel for both transcription factors up to approximately the level of the ββ′ subunits. In nus mutants, the rate of synthesis and the content of the σ subunit were significantly increased. These observations together suggest that the two transcription factors are coordinately regulated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333573
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  • 6
    Electronic Resource
    Electronic Resource
    Histochemical demonstration of sugar residues by lectin and immunocytochemical techniques for blood group antigens in human colon (1987)
    Springer
    Journal of molecular histology 19 (1987), S. 454-464 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Goblet cell mucin in 39 human colons was studied by methods specific for various sugar residues, including staining with three lectins,Dolichos biflorus agglutinin (DBA, specific for blood group A antigen),Griffonia simplicifolia agglutinin-I (GSA-I, B) and peanut agglutinin (PNA, T antigen), and immunostaining for A, B, H and T. Isoantigens A, B or H were found only in the right colon. GSA-I reactive goblet cells occurred in the right colon of both blood group A and B patients and possibly contained isoantigens. However DBA reactive cells were found in all cases. Prior neuraminidase digestion imparted anti-A, GSA-I and DBA reactivities to the cells lining the lower crypts in all cases. This pretreatment also imparted PNA and anti-T reactivities to goblet cells, only the latter reactivity being eliminated by galactose oxidase. Goblet cell mucin in transitional mucosa revealed decreased A and B, and increased H antigens. Enhanced galactose oxidase—Schiff (GOS) and anti-T reactivities were also noted. The present results revealed that some lectin reactions of goblet cells might be related to blood group antigens but others were not, and that different techniques for demonstrating reputedly the same sugar residues produced different results, indicating a need for proper evaluation of their specificity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01675757
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  • 7
    Electronic Resource
    Electronic Resource
    Odor of a papilionid butterfly (1980)
    Springer
    Journal of chemical ecology 6 (1980), S. 867-873 
    Details
    ISSN: 1573-1561
    Keywords: Male scent ; Atrophaneura alcinous ; Lepidoptera ; Papilionidae ; benzaldehyde ; phenylacetaldehyde ; 2-phenylpropenal ; n-heptanal ; 6-methylhept-5-en-2-one ; linalool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Benzaldehyde, phenylacetaldehyde (major component), 2-phenylpropenal,n-heptanal, 6-methylhept-5-en-2-one, and linalool were identified as compounds responsible for the male scent ofAtrophaneura alcinous alcinous. These substances were present predominantly in the wings, and the quantity of them was largest at the inner margin of the hind wing. Female wings also contained some of them in much smaller (except a few components) amounts. The relative proportion of each component exhibited manifests sexual differences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00990470
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  • 8
    Electronic Resource
    Electronic Resource
    History of blood gas analysis. VII. Pulse oximetry (1987)
    Springer
    Journal of clinical monitoring and computing 3 (1987), S. 135-138 
    Details
    ISSN: 1573-2614
    Keywords: Oxygen: saturation ; Measurement techniques: oximetry ; Blood: gas analysis, history
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Medicine
    Notes: Abstract Pulse oximetry is based on a relatively new concept, using the pulsatile variations in optical density of tissues in the red and infrared wavelengths to compute arterial oxygen saturation without need for calibration. The method was invented in 1972 by Takuo Aoyagi, a bioengineer, while he was working on an ear densitometer for recording dye dilution curves. Susumu Nakajima, a surgeon, and his associates first tested the device in patients, reporting it in 1975. A competing device was introduced and also tested and described in Japan. William New and Jack Lloyd recognized the potential importance of pulse oximetry and developed interest among anesthesiologists and others concerned with critical care in the United States. Success brought patent litigation and much competition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00858362
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  • 9
    Electronic Resource
    Electronic Resource
    Hierarchical program specification and verification — a many-sorted logical approach (1980)
    Springer
    Acta informatica 14 (1980), S. 135-155 
    Details
    ISSN: 1432-0525
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Summary The notion of abstractions in programming is characterized by the distinction between specification and implementation. As far as the specification structures are concerned, hierarchical program development with abstraction mechanisms is naturally regarded as a process of theory extensions in a many-sorted logic. To support such program development, a language called t is proposed with which one can structuredly build up theories and write their program implementation. There, the implementation is regarded as another level of theory extension, and the relation between the specification and the implementation of an abstraction is characterized in terms of a homomorphism between the two theories. On this formalism, a mechanizable proof method is introduced for validation of implementations of both data and procedural abstraction. Finally, a new data type concept is introduced to generalize the so-called type-parametrization mechanism. A justification of this concept within the first order logic is provided as well as its applications to program structuring and verification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288541
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  • 10
    Electronic Resource
    Electronic Resource
    Late development and fertility of adult worms derived from gamma-irradiated first-stage larvae of Angiostrongylus cantonensis (1987)
    Springer
    Parasitology research 73 (1987), S. 159-164 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Effects of gamma ray irradiation on the first-stage larvae of Angiostrongylus cantonensis were studied. Compared to the non-treated controls, infection of rats with third-stage larvae which developed from irradiated first-stage larvae resulted in reduced recovery rates of adults. There was also a change in the male-female adult worm ratio and a reduction in larval output per female in relation to increasing irradiation dosage. Morphological changes in the adults were noted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00536473
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