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  • ASTROPHYSICS  (324)
  • ENERGY PRODUCTION AND CONVERSION  (210)
  • Cell & Developmental Biology
  • 1980-1984  (619)
  • 1960-1964
  • 1950-1954
  • 1984  (309)
  • 1980  (310)
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  • 1980-1984  (619)
  • 1960-1964
  • 1950-1954
Year
  • 1
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2011-08-18
    Description: A technique has been developed for coating low-cost mullite-based refractory substrates with thin layers of solar cell quality silicon. The technique involves first carbonizing one surface of the ceramic and then contacting it with molten silicon. The silicon wets the carbonized surface and, under the proper thermal conditions, solidifies as a large-grained sheet. Solar cells produced from this composite silicon-on-ceramic material have exhibited total area conversion efficiencies of ten percent.
    Keywords: ENERGY PRODUCTION AND CONVERSION
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  • 3
    Publication Date: 2019-06-28
    Description: Nine X-ray outbursts from the LMC have been observed with the HEAO 1 Large-Area Sky Survey Instrument. Some are shown to originate in the recurrent transient A0538-66, confirming the proposed 16 day periodicity and showing that the duration of the events can be as long as 14 days or as short as a few hours. Deviations from precise periodicity can be attributed to phase jitter or to a change in period occurring around the time of an exceptionally long outburst. Other outbursts which are irregular and consistently shorter originate in LMC X-4. A long-term light curve indicates that the LMC X-4 outbursts occur only when the source is in a high state, but are not strongly correlated with the binary phase.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal; vol. 240
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  • 4
    Publication Date: 2019-06-27
    Description: The paper presents a 1-10-keV survey of distance class 4, 5, and 6 Abell clusters using the HEAO 1 NRL large area survey experiment. The survey is a little less than 1/30 of the Abell catalog. Eleven clusters are identified with X-ray sources, and X-ray upper limits are provided for 60 others.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal; vol. 235
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  • 5
    Publication Date: 2019-06-27
    Description: The paper deals with spectrophotometric observations covering the essentially complete wavelength interval between 1.2 and 30.0 microns. The observations confirm the identification of the C3 band at 5.2 microns. They show that if SiC2 is present, the SiC1 absorption band at 5.7 microns would be obscured by C3 at a 1% spectral resolution. Silicon carbide emission at 11.5 microns exists simultaneously with C3 absorption at 5.2 microns, requiring a contribution of both species to the violet opacity of Y CVn.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal; vol. 235
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  • 6
    Publication Date: 2019-06-28
    Description: Ultraviolet spectra of comet Austin (1982) obtained in July 1982 at heliocentric distances ranging from 1.10 to 0.81 AU preperihelion with the IUE reveal that the comet is very similar in chemical composition and appearance to comet Bradfield (1979X). In addition, the derived H2O production rate is found to vary with heliocentric distance as r to the -3.6, similar to the r to the -3.7 behavior found for comet Bradfield. It is pointed out, however, that the limited sample rate precludes the observation of the short-term variations which could be produced by a rotating nonuniform cometary nucleus.
    Keywords: ASTROPHYSICS
    Type: Astronomy and Astrophysics (ISSN 0004-6361); 131; 2 Fe
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  • 7
    Publication Date: 2019-07-13
    Description: This paper presents an overview of photovoltaic systems applications since the initiation of the U.S. National Photovoltaic Program in 1975. Experiences with these applications are summarized and some conclusions are drawn. Implications for future research, technology development and application experiments are drawn from the experiences to date.
    Keywords: ENERGY PRODUCTION AND CONVERSION
    Type: Photovoltaic Specialists Conference; Jan 07, 1980 - Jan 10, 1980; San Diego, CA
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  • 8
    Publication Date: 2019-06-28
    Description: The present mapping of the 3P1-3P2 fine structure line emission from neutral atomic oxygen near the galactic center shows the emission to be extended over more than 12 pc along the galactic plane, centered on the position of Sgr A West. The rotational velocity of the O I gas at R of about 1 corresponds to a mass within the central parsec of about 3 million solar masses. The forbidden O I line probably arises in a predominantly neutral atomic region immediately outside the ionized central parsec of the Galaxy. Gas temperatures are greater than 100 K, and the total integrated luminosity radiated in the line, which is about 100,000 solar luminosities, substantially contributes to the cooling of the gas. The 3P1-3P0 fine structure line of the O III forbidden line has also been detected at 88 microns toward Sgr A West, coming from high density ionized gas.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 1 (ISSN 0004-637X); 276; 551-559
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 29-35 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This study describes intercellular bridges in the ovaries of neonatal gerbils. Electron microscopy has revealed the presence of true intercellular bridges, connecting oogonia or oocytes, in ovaries of newborn gerbils. The cytoplasm of the intercellular channels is similar to that of the connected cells, with mitochondria, smooth and rough endoplasmic reticulum, and free ribosomes present. Lysosomes are also occasionally present in the intercellular bridges and they may be involved in early waves of oocyte atresia. An electrondense substance, 350-500 Å thick, is located immediately beneath the unit membrane of the intercellular bridges. Accumulation of electron-dense material increases the thickness of the walls of the intercellular bridges, supporting and maintaining the patency of the channels. It is suggested that the intercellular channels probably allow the interchange of nutrients, organelles, and possibly regulatory materials as well.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 243-252 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A small short muscle frequently acts across a joint in parallel with a vastly larger and longer muscle; therefore it should play a minimal role in the mechanical control of that joint. This study provides evidence suggesting that the small member of such a “parallel muscle combination” (PMC) may serve an important sensory feedback role. The spindle densities of large and small members of PMCs in man and the dog were determined and compared. Epaxial PMCs controlling canine intervertebral joints were dissected and tissue samples were embedded in paraffin, sectioned transversely to the muscles' long axis and, stained with hematoxylin-periodic acid-Schiff (PAS). Representative tissue sections were projected on to stereological grids and the percentage volume of spindles was determined. Data existing in the literature were used to ascertain spindle densities in human PMCs controlling joints in the cervico-occipital region and the extremities. The spindle density for each muscle in a group of PMCs controlling a particular motion was listed, and the mean spindle densities were determined for both the large and the small members of the group. Student's unpaired t test was used to determine the significance of the differences between mean spindle densities. Linear regression was calculated and the data were plotted graphically.In all PMCs examined, the spindle density of the small muscles was significantly higher than that of their large counterparts. It is therefore proposed that the small muscles of PMCs may function as “kinesiological monitors” generating important proprioceptive feedback to the central nervous system.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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