ISSN:
1612-1112
Keywords:
Clinical dextran
;
Rapid GPC
;
Molecular weight distribution
;
Schulz-Zimm distribution
;
Hydroxyethyl starch
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Clinical dextran can be fractionated by rapid gel-permeation chromatography on SpheronR with the aid of a high-performance liquid chromatograph. The linear calibration function is obtained by dextran fractions whose molecular weights Mw and Mn are determined by absolute methods (light scattering, membrane osmometry) and end group analysis. The transformation of elution diagrams into a molecular weight distribution is described. Characteristic data for dextran 40 and 70 were obtained from the integral distribution curves. The molecular weight distribution of dextran fractions was found to fit a Schulz-Zimm distribution which is typical for long chain polymers. For quality control purposes clinical dextran can be characterized by its molecular weight distribution with high accuracy within one hour.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02314870
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