ISSN:
1432-072X
Keywords:
Neurospora crassa
;
Slime variant
;
Vacuoles
;
Isolation
;
Hydrolases
;
Arginine permease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described. Isolated vacuoles are characterized by 12 to 50 times increased specific activities of several hydrolases as compared with the total homogenate of protoplasts. Total α-amino nitrogen, arginine, and polyphosphate are also greatly enriched in these vacuoles. Vacuoles are equipped with a permease for the transport of basic amino acids across the tonoplast.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00413268
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