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  • Abscisic acid  (6)
  • Springer  (6)
  • Periodicals Archive Online (PAO)
  • 1980-1984
  • 1975-1979  (6)
  • 1955-1959
  • 1935-1939
  • 1979  (6)
Collection
Keywords
Publisher
  • Springer  (6)
  • Periodicals Archive Online (PAO)
Years
  • 1980-1984
  • 1975-1979  (6)
  • 1955-1959
  • 1935-1939
Year
  • 1
    Electronic Resource
    Electronic Resource
    Uptake and distribution of abscisic acid in Commelina leaf epidermis (1979)
    Springer
    Planta 144 (1979), S. 167-172 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Commelina ; Epidermal strip ; Stomatal closure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Closure of stomata by abscisic acid (ABA) was studied by floating leaf epidermal strips of Commelina communis L. in PIPES buffer (pH 6.8) containing a range of KCl concentrations. Control apertures were greatest at high concentrations of the salt, and the effects of ABA, in terms of closure, were most pronounced below 100 mol m-3 KCl. Stomata opened on strips floated on buffer plus 50 mol m-3 KCl and closed within 10 min when transferred to the same medium plus 0.1 mol m-3 ABA. [2-14C]ABA was used to study uptake and distribution of the hormone by the epidermal strips. It was calculated that no more than 6 fmol ABA were present per stomatal complex at the time of closure, although uptake continued thereafter. Microautoradiography indicated that radioactivity from [2-14C]ABA accumulated in the stomatal complex at or near the guard cells within 20 min. TLC was used to examine the state of the label after 1 h incubation. Efflux of label from preincubated tissue appeared to occur in three phases (t1/2=7.2 s, 4.0 min, 35.2 min). Efflux was correlated with stomatal re-opening. The results confirm that ABA can accumulate in the epidermis of C. communis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387266
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  • 2
    Electronic Resource
    Electronic Resource
    Abscisic acid, abscisic acid-esters and phaseic acid in vegetative terminal buds of Acer pseudoplatanus during emergence from winter dormancy (1979)
    Springer
    Planta 146 (1979), S. 591-596 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Acer ; Bud dormancy ; Dormancy (bud)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Levels of free-abscisic acid and “boundabscisic acid” (alkaline hydrolyzable abscisic acidesters) in replicated samples of terminal vegetative buds of sycamore trees were measured during natural emergence from winter dormancy by gas chromatographic methods together with isotope dilution estimation of recovery rates. Not until after the buds had been released from true dormancy in January by winter chilling did any clear change occur in either abscisic acid (ABA) fraction, or in total ABA, on any basis of comparison. The percentage of total ABA present as the free acid declined at the end of true dormancy to approximately two-thirds of its value in the earlier winter months. It is concluded that glucosylation of ABA is unlikely to play a major part in the mechanism of release from dormancy in vegetative sycamore buds. At the end of true dormancy there was a large transient increase in what appeared to be phaseic acid, but this was not accompanied by any marked decrease in either free- or bound-ABA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388837
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  • 3
    Electronic Resource
    Electronic Resource
    Sensitivity of Commelina stomata to abscisic acid (1979)
    Springer
    Planta 146 (1979), S. 623-628 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Commelina ; Microautoradiography ; Stomatal sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Stomata of Commelina leaves pre-opened by incubation in moist air were found to close within 30 min when supplied with abscisic acid (ABA) via the transpiration stream. Radioactive ABA had similar effects, but allowed the distribution of the compound within the leaf to be measured and correlated with stomatal movements to give estimates of the sensitivity of Commelina stomata. On a whole-leaf basis, less than 163 fmol ABA per mm2 leaf area were present at the time of complete stomatal closure. This was close to other published estimates. By taking epidermal 14C measurements, however, it was possible to increase the accuracy of the estimate on the assumption that only ABA present in the epidermis was physiologically active. Thus, less than 235 amol ABA for stomatal complex were present at complete closure, and statistically significant narrowing of the stomatal aperture had occurred when between 12.6 and 45.4 amol per complex were present. The distribution of ABA within the epidermal tissue after transpiration-stream application was studied using microautoradiography, and the compound appeared to have accumulated within the stomatal complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388842
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  • 4
    Electronic Resource
    Electronic Resource
    Mannanase production by the lettuce endosperm (1979)
    Springer
    Planta 144 (1979), S. 333-340 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Endosperm ; Germination (seeds) ; Lactuca ; Mannanase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Endo-β-mannanase (EC 3.2.1.78) is produced and secreted by the cells of the endosperm of lettuce (lactuca sativa L.) “seeds” (achenes). In imbibed intact seeds, production is prevented by inhibitors. If the endosperm is incubated alone, these inhibitors can be removed by leaching, allowing mannanase production. Abscisic acid, a component of lettuce seeds, inhibits the production of mannanase in the isolated endosperm, and may be involved in regulation of mannanase production in intact seeds. During germination the inhibition is removed, beginning 4–8 h after red-light irradiation, which was given 4 h from sowing. The cotyledons participate in this process, and are controlled by events occuring in the axis within 4 h from red-light irradiation. This control by the axis apparently depends on the exchange of diffusible substances. Both benzyladenine and gibberellic acid can replace the influence of the axis if the latter is removed, and may therefore be involved in the control by the axis of the rest of the seed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391576
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  • 5
    Electronic Resource
    Electronic Resource
    Abscisic acid levels and metabolism in the leaf epidermal tissue of Tulipa gesneriana L. and Commelina communis L. (1979)
    Springer
    Planta 146 (1979), S. 135-138 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Commelina ; Leaf epidermal tissue ; Radioimmunoassay (ABA) ; Tulipa ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have shown the presence of abscisic acid (ABA) in abaxial epidermal strips taken from Tulipa gesneriana and Commelina communis and that the ABA level rises in the epidermis when leaves are water stressed. ABA levels had risen 50% in the abaxial epidermis of C. communis 30 min after the leaves lost 10% of their fresh weight. Epidermis from both T. gesneriana and C. communis metabolize [14C]ABA to several products probably including phaseic acid (PA) and dihydrophaseic acid (DPA).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388223
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  • 6
    Electronic Resource
    Electronic Resource
    Abscisic-acid and gibberellin action in developing kernels of triticale (cv. 6A190) (1979)
    Springer
    Planta 146 (1979), S. 249-255 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Amylase ; Cereal kernel ; Germination (seeds) ; Gibberellin ; Triticale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Abscisic-acid (ABA) levels were determined in triticale 6A190 kernels at various stages of development from anthesis to maturity. ABA reached a maximum at ca. 22 d post-anthesis and declined rapidly 12 d later. Associated with drying of the kernel at maturity there was a rapid increase in the endogenous level of α-amylase, apparently based upon de-novo synthesis. Simultaneously there were visible signs of degradation of the large starch grains in the starchy endosperm. Regulation of α-amylase production in the kernel by exogenous gibberellic acid (GA3) was only evident in the almost mature kernel (30–40 d after anthesis) and then only if these kernels were first dried artificially. Furthermore, little α-amylase mRNA could be detected prior to kernel maturity and water loss. Thus, the high levels of gibberellin (GA) that have been found early in kernel development in cereals do not appear to control the later production of α-amylase and onset of kernel germination in the ear of triticale. However, the presence of high levels of ABA until maturity could prevent early germination and premature production of α-amylase. Kernels of triticale 6A190 are characteristically shrivelled and non-dormant at maturity. The relevance of changes in the capacity of kernels to respond to and produce GA and ABA is discussed in relation to problems of harvest dormancy in cereals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387794
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