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  • dihaploid  (3)
  • Compartmentation  (1)
  • Chemistry
  • Springer  (4)
  • 1975-1979  (4)
  • 1978  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Localization of polyphosphate in vacuoles of Saccharomyces cerevisiae (1978)
    Springer
    Archives of microbiology 116 (1978), S. 275-278 
    Details
    ISSN: 1432-072X
    Keywords: Yeast ; Polyphosphate ; Compartmentation ; Vacuole ; Cell wall
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Virtually all of the polyphosphate (PP) present in yeast protoplasts can be recovered in a crude particulate fraction if polybase-induced lysis is used for disrupting the protoplasts. This fraction contains most of the vacuoles, mitochondria and nuclei. Upon the purification of vacuoles the PP is enriched to the same extent as are the vacuolar markers. The amount of PP per vacuole is comparable to the amount of PP per protoplast. The possibility that PP is located in the cell wall is also considered. In the course of the incubation necessary for preparing protoplasts, 20% of the cellular PP is broken down. As this loss of PP occurs to the same extent in the absence of cell wall degrading enzymes, it is inferred that internal PP is metabolically degraded, no PP being located in the cell walls. It is concluded that in Saccharomyces cerevisiae most if not all of the PP is located in the vacuoles, at least under the growth conditions used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417851
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  • 2
    Electronic Resource
    Electronic Resource
    Inheritance of genetic markers from two potato dihaploids and their respective parent cultivars (1978)
    Springer
    Euphytica 27 (1978), S. 681-688 
    Details
    ISSN: 1573-5060
    Keywords: Potato ; dihaploid ; marker genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the first inbred generation (I1) of cv. Black 4495 a dark green, slowly growing mutant (coded ds) was found, whereas the I1 of its self-compatible dihaploid, B16, comprised this ds mutant and in addition the mutants virescens (v) and yellow margin (ym). The occurrence of ds and ym might trace back to diploid S. phureja, one of the ancestors of Black 4495. No lethal mutants were observed in I1 of B16. Analysis of I1 of cv. Gineke revealed a simplex condition for virescence and either duplex or triplex heterozygosity for one lethal gene. On the other hand, the I1 of its dihaploid, G254, segregated for virescence and for three different lethal genes. It is shown that both in B16 and in G254 homozygosity of an S-bearing translocation causes early death of embryo and endosperm, thus preventing seed development. From this study it appeared that the three lethal genes from G254 affect germination rate of the seeds. The genotypes at 11 loci of B16 and G254 are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023703
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  • 3
    Electronic Resource
    Electronic Resource
    Genetics of self-compatibility in dihaploids of Solanum tuberosum L. 4. Linkage between an S-bearing translocation and a locus for virescens (1978)
    Springer
    Euphytica 27 (1978), S. 381-384 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; self-compatibility ; dihaploid ; linkage ; virescens ; translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three dihaploids of Solanum tuberosum (two self-compatible, one self-incompatible) were found to be heterozygous for a monogenic recessive virescent mutant. Intercrossing resulted in the expected 3 : 1 ratio only in crosses involving one self-compatible and one self-incompatible parent. Self-compatible x self-compatible matings produced F1's in which 6:1 was found. The same ratio was observed in the self progeny of the two self-compatible dihaploids. This significant deviation could be explained by assuming linkage (25% crossing-over) between v and an S-bearing translocation. This translocation causes self-compatibility in the dihaploids used and early lethality when homozygous.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043162
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  • 4
    Electronic Resource
    Electronic Resource
    Genetics of self-compatibility in dihaploids of Solanum tuberosum L. 5. The S-genotype of tetraploid potato cultivar gineke (1978)
    Springer
    Euphytica 27 (1978), S. 385-388 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; incompatibility ; dihaploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Investigations of the genetics of self-compatibility and self-incompatibility in dihaploids and diploid derivatives from cv. Gineke revealed the presence of S 1, S2 and S 3 at the S-locus of Gineke and in addition an S 1-allele on a translocation. By means of a complete tester set involving the S-alleles S 1, S2 and S 3 (all from Gineke) and S 4 (from Black 4495) it was demonstrated that some Gineke dihaploids were compatible with all six testers. This indicated a fourth S-allele in Gineke, which differs from those in the tester series and was therefore assigned S 5. Additional evidence was obtained from an analysis of F1's from crosses of two S 5-bearing dihaploids and one of the testers. So the S-genotype of cv. Gineke was identified as S 1S2S3S5/S1, the second S 1 being the S-allele on a translocated fragment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043163
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