ISSN:
1617-4623
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary The inactivation of λ repressor was followed by the specific DNA binding assay during the course of lysogenic induction provoked by incubation at 42°C of an E. coli tif-1 lysogenic strain. The presence of up to 400 μg/ml chloramphenicol during the inducing treatment did not impair the loss of repressor binding activity, whilst concentrations of 200 μg/ml neomycin and 100 μg/ml rifampicin effectively inhibited the inactivation of λ repressor. Residual protein synthesis in the presence of chloramphenicol, neomycin and rifampicin was 5%, 5% and 27% respectively of that observed in the drug-free control. This residual synthesis did not appear to involve amplification of the X-protein. These results suggest that tif-mediated inactivation of the λ repressor requires the activation of some specific gene(s), the translation of which appears to be resistant to chloramphenicol.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00271958
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