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  • isozymes  (6)
  • Springer  (6)
  • Annual Reviews
  • 1980-1984  (4)
  • 1975-1979  (2)
  • 1984  (4)
  • 1978  (2)
Collection
Keywords
Publisher
  • Springer  (6)
  • Annual Reviews
Years
  • 1980-1984  (4)
  • 1975-1979  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Three alcohol dehydrogenase genes in wild and cultivated barley: Characterization of the products of variant alleles (1984)
    Springer
    Biochemical genetics 22 (1984), S. 495-515 
    Details
    ISSN: 1573-4927
    Keywords: alcohol dehydrogenase ; isozymes ; barley ; anaerobic induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Barley (Hordeum vulgare) and its wild progenitor (H. spontaneum) have three loci for alcohol dehydrogenase (EC 1.1.1.1; ADH). The Adh1 locus is constitutively expressed in seed tissues, whereas expression of the loci Adh2 and Adh3 requires anaerobic induction. The Adh3 gene is well expressed in aleurone and embryo tissues kept under N2 for 2–3 days. Using N2-treated embryos, a diverse collection of H. spontaneum was screened in starch gels for electrophoretic variants at the Adh3 locus. Four variants were found: two were conventional mobility variants (Adh3 S, Adh3 V); one was a null variant (Adh3 n); and the fourth (Adh3 I) variant lacked active homodimers and showed reduced heterodimer activity. The 35S-labeled monomers induced under N2 in the lines homozygous for Adh1, Adh2, or Adh3 variants were immunoprecipitated with antiserum raised against maize ADH. Fluorography after separation by SDS-PAGE and by urea-isoelectric focusing indicated that the Adh3 n allele was CRM- and that the Adh3 I gene product was smaller than normal. The Adh1 and Adh3 variants showed independent segregation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484519
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  • 2
    Electronic Resource
    Electronic Resource
    Heterozygosity in Yellowstone Park elk, Cervus canadensis (1978)
    Springer
    Biochemical genetics 16 (1978), S. 651-657 
    Details
    ISSN: 1573-4927
    Keywords: isozymes ; mammals ; heterozygosity ; polymorphism ; elk
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Protein products of 24 loci from the genomes of Yellowstone Park elk were analyzed by electrophoresis. Heterozygosity was detected in only one system, making elk much less polymorphic than eastern whitetailed deer. Data for several other large mammals are compared with those for elk and reveal similarly low levels of isozymic variation. The data are consistent with the fine-grained niche theory but difficult to reconcile with bottlenecks and genetic drift.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484721
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  • 3
    Electronic Resource
    Electronic Resource
    Genetics of allozyme variants and linkage groups in lentil (1984)
    Springer
    Euphytica 33 (1984), S. 329-336 
    Details
    ISSN: 1573-5060
    Keywords: Lens culinaris ; lentil ; Lens orientalis ; isozymes ; genetics ; linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetics of 8 electrophoretically detectable enzymes in lentil was examined. The enzyme systems glutamic-oxaloacetic transaminase, malic enzyme, phosphoglucomutase, alcohol dehydrogenase, 6-phosphogluconate dehydrogenase, shikimic dehydrogenase and isocitrate dehydrogenase were assayed. The allozymes at each of the studied loci behaved in a codominant manner and segregated in the expected Mendelian fashion. Linkage tests between these loci and an additional morphological trait revealed two linkage groups that involved 5 loci, the rest were independent of each other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021129
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  • 4
    Electronic Resource
    Electronic Resource
    Inheritance and transfer of multiple-flower character from Capsicum chinense into Capsicum annuum (1984)
    Springer
    Euphytica 33 (1984), S. 769-777 
    Details
    ISSN: 1573-5060
    Keywords: Capsicum annuum ; Capsicum chinense ; pepper ; genetics ; multiple-flowers ; inheritance ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Capsicum annuum L. produces a single flower and thus a single fruit per branching node. In contrast, Capsicum chinense Jacq. yields two or more flowers per node. If genes for multiple-flowers per node could be transferred from C. chinese to C. annuum, it might be possible to breed C. annuum varieties with a more concentrated fruit set and potentially higher yield. Using progeny from an interspecific cross between C. annuum cv. NM 6-4 and C. chinense CA4, it has been determined that a minimum of five independently segregating chromosomal regions control the difference in flowering behavior between these two accessions. One of the segments is located on a translocated chromosome. Epistatic interactions among independent chromosomal regions appear to play a major role in the determination of this trait. Although it is unlikely that the multiple-flower character can be transferred to C. annuum in its full intensity while maintaining a horticulturally-acceptable phenotype, it may be possible to breed varieties which produce an average of more than 1.5 flowers per node, a value 50% higher than existing C. annuum varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021903
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic and molecular analysis of nonrandom dimer assembly of the creatine kinase isozymes of fishes (1978)
    Springer
    Biochemical genetics 16 (1978), S. 811-829 
    Details
    ISSN: 1573-4927
    Keywords: isozymes ; creatine kinase ; fishes ; dimer ; restricted assembly
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Species within many families of actinopterygian bony fishes (class Osteichthyes) have a two-banded allelic isozyme phenotype in individuals heterozygous at the creatine kinase A locus. This two-banded pattern is formed by the presence of the two homodimeric isozymes and the absence of the expected heterodimer. Sharks and amphibians have retained the ability to form all three allelic isozymes in individuals which are heterozygous. Reversible denaturation procedures were able to assemble the different allelic CK-A subunits within a species to form CK-A2 heterodimers. Furthermore, heterodimers were formed from different CK-A subunits from highly divergent species after this in vitro molecular hybridization process. It is concluded from these studies that the polypeptidebinding sites of creatine kinase are structurally conservative in most fishes and that the absence of a heterodimer in heterozygous individuals is not due to a structural incompatibility between the different A subunit types or to an instability of the heterodimer during electrophoresis. A temporal and/or spatial isolation of allelic CK-A subunit synthesis and assembly, within differentiated skeletal muscle, appears to have evolved in the actinopterygian bony fishes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484738
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  • 6
    Electronic Resource
    Electronic Resource
    Electrophoretic analysis of the phylogeny of the self-pollinating populations ofClarkia xantiana (1984)
    Springer
    Plant systematics and evolution 147 (1984), S. 91-102 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Onagraceae ; Clarkia xantiana ; Electrophoresis ; isozymes ; phylogenetic models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electrophoretic variation in isozymes coded by 40 loci was examined in two self-pollinating populations, one with white and the other with pink flowering plants, and four outcrossing populations ofClarkia xantiana (Onagraceae) native to California. The study was carried out to test theMoore & Lewis (1965) hypothesis that the pink selfer originated from the sympatric outcrossing population and then gave rise to the white selfer. The hypothesis could be rejected if one or the other selfer was more similar genetically to an allopatric population than to the sympatric one. Both selfers were monomorphic at all loci whereas the outcrossing populations were polymorphic at nearly half of them. The two selfers had the same genes at 32 loci but had different ones at eight loci. The pink selfer was not more similar to the allopatric populations than to the sympatric one, consistent with theMoore & Lewis model. The evidence also supported their proposal that the white selfer originated from the pink one and not independently. The electrophoretic evidence was valuable because it permitted qualitative comparisons (presence versus absence of particular alleles) between the selfer and the several outcrossing populations; such analysis was not previously possible because the latter populations are morphologically and cytologically similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984582
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