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  • 1
    Electronic Resource
    Electronic Resource
    Morphological alterations and functional changes of interhepatocellular junctions induced by bile duct ligation (1977)
    Springer
    Cell & tissue research 182 (1977), S. 299-310 
    Details
    ISSN: 1432-0878
    Keywords: Liver ; Bile duct ligation (rat) ; Cell membrane ; Intercellular junctions ; Ultrastructure ; Freeze-fracturing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of bile duct ligation on the intercellular junctions of hepatocytes was investigated. The features and the arrangement of the bile canaliculi and the zonulae occludentes alter concomitant to the increase of the intracanalicular pressure. The lumen of the bile canaliculi enlarges and the microvilli disappear. The array of the zonulae occludentes becomes irregularly shaped, the number of strands diminishes and interruptions of the strands occur. With peroxidase a leakage in the bile-blood barrier is detected. Furthermore a disappearance of gap junctions between the hepatocytes after bile duct ligation is observed. The present investigation shows that the zonulae occludentes are mobile structures which are changed by increased unilateral pressure. Due to their ultrastructural alterations, a leakage of the permeability barrier between physiological compartments is found. We acknowledge the helpful criticism and discussion of Prof. H.D. Fahimi. We are indebted to Mrs. B. Brühl, M. Bürkle and Ch. Walenta for technical assistance, and to stud. med. Jon Greenberg for preparing the manuscript
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219766
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural identification of a new cell type — the N-cell as the source of neurotensin in the gut mucosa (1977)
    Springer
    Cell & tissue research 184 (1977), S. 445-452 
    Details
    ISSN: 1432-0878
    Keywords: Tupaia belangeri (Primate) ; Small intestine ; Neurotensin ; Immunohistochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neurotensin-cell is identified immunohistochemically and ultrastructurally by differential counting of endocrine cells in the gut of a primate (Tupaia belangen). Utilizing light microscopy, the EC-cells are identified by the Masson-Fontana silver stain; with the same method the neurotensin cells are not stained. The other endocrine cells have been quantified in the small intestine using the peroxidase-antiperoxidase stain with antisera against glucagon, somatostatin, cholecystokinin, gastrin, secretin, pancreatic polypeptide, gastric inhibitory peptide and neurotensin. In the ileal mucosa of Tupaia, the most frequent endocrine cell is the EC-cell followed by the glucagonoid cell, (L-cell). The immunoreactive neurotensin cell represents the third most frequent endocrine cell in this region. On the ultrastructural level, this third most frequent endocrine cell is a heretofore undescribed cell, the N-cell, containing electron dense secretory granules measuring 335±87 nm in diameter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220968
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  • 3
    Electronic Resource
    Electronic Resource
    The effects of glycerol treatment on the isolated rat heart (1977)
    Springer
    Cell & tissue research 185 (1977), S. 43-62 
    Details
    ISSN: 1432-0878
    Keywords: Isolated perfused heart (rat) ; Glycerol treatment ; Ultrastructure ; Electrolytes ; Inulin space
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Isolated rat hearts were perfused with a balanced electrolyte solution containing 1000mM glycerol for 15min and then perfused with normal electrolyte solution for up to 32 min. The perfusion with hypertonic glycerol solution and subsequent washout is termed “glycerol treatment”. Initially, glycerol removal causes swelling and rupture of the T-system in ventricular myocardial cells which correlates temporally with a period of cardiac arrest. Contractility returns during further glycerol removal and concomitant recovery of the T-system is observed. Atomic absorption spectometry and neutron activation analysis were used to measure ventricular sodium, potassium and calcium ion content. There is no apparent correlation between changes in ion content and cardiac arrest or recovery. The water movements were calculated from wet weight, dry weight and inulin space, and confirmed by morphometric analysis of extracellular and intracellular space. It is suggested that the swelling and rupture of the T-system is due to the rapid water movements that were observed during the onset of glycerol removal. Ultrastructural analysis of glycerol-treated atrium from the same hearts shows damage of mitochondria and of the L-system and intracellular edema. The structural changes are correlated with a loss of atrial contraction. As in ventricular myocardium, resumption of contraction is associated with an almost complete recovery from ultrastructural damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226667
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  • 4
    Electronic Resource
    Electronic Resource
    Three dimensional behavior of negative electron resists (1977)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 17 (1977), S. 377-380 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: It is illustrated experimentally that swelling and deformation of narrow lines (width 〈 1 μm) is an important limitation on the fidelity and transverse resolution of negative electron resists. The thickness response curve developed for negative resists is discussed and the effect of feature size is introduced through the experimental parameter DgR, the rigid gel dose, or the dose required to initiate a free standing line of width 〈 0.2 μm. The transition from line to area exposure is discussed and the effect of substrate backscatter or transverse resolution in gratings is calculated. The observed resolution is 3-5 times the calculated value from swelling and deformation during development.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760170609
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  • 5
    Electronic Resource
    Electronic Resource
    Powder processing of ultra-high molecular weight polyethylene I. Powder characterization and compaction (1977)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 17 (1977), S. 21-26 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Powders of ultra-high molecular weight polyethylene from three suppliers were characterized for density, crystallinity, particle size, particle size distribution and particle morphology. The powders had molecular weights in the range 2-5 million. Bulk powder behavior, compressibility, green strength and springback were evaluated and explained in terms of particle characteristics. The green densities of the powders were found to reach a plateau at pressures of about 100 MPa. These plateau density levels were found to depend upon powder, characteristics and to lie between 80 and 90 percent relative density. Green strength is shown to be a unique function of a densification parameter.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760170104
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