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  • Ultrastructure  (8)
  • Springer  (8)
  • Nature Publishing Group
  • 2010-2014
  • 1980-1984
  • 1975-1979  (8)
  • 1940-1944
  • 1976  (8)
Collection
Publisher
  • Springer  (8)
  • Nature Publishing Group
Years
  • 2010-2014
  • 1980-1984
  • 1975-1979  (8)
  • 1940-1944
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 171 (1976), S. 305-315 
    ISSN: 1432-0878
    Keywords: Chorioallantoic placenta ; Gap junctions ; Diaplacental transport ; Ultrastructure ; Freeze-fracturing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Gap junctions were found to be a constant feature of chorioallantoic placentae with two or three trophoblastic layers. The gap junctions connect layers I and II in hemodichorial and layers II and III in hemotrichorial placentae. Although the gap junctions vary in form and in the packing density of membrane-associated particles, they cover an extensive surface area in all species examined. The gap junctions always connect adjacent membranes of two trophoblastic layers, which show no evidence of micropinocytotic activity; at least one of these trophoblastic layers is syncytial. It is therefore concluded that the gap junctions play an important role in diaplacental transport. We consider that gap junctions act as molecular sieves, resulting in limitations in the transport of large molecules. The passage of small molecules, on the contrary, would be facilitated by the gap junctions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 173 (1976), S. 465-482 
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Cytochemistry ; Oocytes ; Molluscs ; Vitellogenesis ; Nucleoli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ovarian oocytes of Agriolimax reticulatus (Müller) have been studied by light and electron microscopy and electron cytochemistry. The development of the oocyte in the ovotestis may be divided into three stages. During Stage I the oocyte cytoplasm contains mainly ribosomes and also strands of endoplasmic reticulum, scattered mitochondria and Golgi systems. The nucleus contains both a paranucleolus and an eunucleolus. By Stage II the oocyte has enlarged, especially in a plane parallel to the basement membrane. In addition to the above mentioned organelles, the cytoplasm contains lipid, glycogen and early yolk platelets. During Stage III, the oocyte continues to enlarge, but mainly in a plane perpendicular to the basement membrane. A considerable degree of cytoplasmic differentiation has also taken place. The plasma membrane of the oocyte has become specialized with the appearance of a polysaccharide-rich glycocalyx, microvilli and pinocytotic tubules. Elsewhere, much of the background cytoplasm, containing Golgi-derived, polysaccharide and acid phosphatase-rich multivesiculate bodies, lipid and glycogen, is sequestered by smooth membranes and ultimately fuses with the growing yolk platelets. The nucleus contains an amphinucleolus, characteristic of many gastropods. The findings of this study are discussed in relation to results from other studies on oogenesis.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 168 (1976), S. 261-270 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Exercise ; Rat ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Four groups of male rats were exercised for periods of 2, 4, 6, and 8 weeks with controls in each group. As a result of chronic exercise there was an increase in the width of the zona fasciculata of the adrenal cortex. Also, there was an increase in the number and size of the mitochondria, and an increase in the quantity of smooth endoplasmic reticulum, and during the first 4 weeks of exercise an increase in the number of lipid droplets in the zona fasciculata. The close relationship between the smooth endoplasmic reticulum and the mitochondria, and the relationship between the smooth endoplasmic reticulum and the lipid droplets suggests a possible means for a transport mechanism for movement of precursors between these organelles.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 166 (1976), S. 497-510 
    ISSN: 1432-0878
    Keywords: Spermatogenesis ; Phosphatase cytochemistry ; Ultrastructure ; Testis ; Male reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic cytochemistry was used to determine the localization of five phosphatase enzymes—glucose-6-phosphatase, inosine diphosphatase, thiamine pyrophosphatase, acid phosphatase, and adenosine triphosphatase—in control human testes. Glucose-6-phosphatase occurred in the endoplasmic reticulum and nuclear envelope of Sertoli cells, Leydig cells and primitive spermatogonia, but was not observed in more advanced spermatogenic cells. The presence of glucose-6-phosphatase activity paralleled the presence of glycogen in spermatogenic cells, i.e., both occurred in type AL and AD spermatogonia but not in type AP or B spermatogonia or in more advanced spermatogenic cells. Inosine diphosphatase activity was found in the endoplasmic reticulum, nuclear envelope, and Golgi complex of Sertoli cells and all spermatogenic cells except late spermatids. Additionally, inosine diphosphatase activity was localized at the junctions between Sertoli cells and late spermatids, but was not associated with any other plasma membrane. Thiamine pyrophosphatase reaction product was found in the Golgi bodies of Sertoli cells and in spermatogenic cells through immature spermatids. Neither inosine diphosphatase nor thiamine pyrophosphatase was observed in the Golgi bodies of spermatids during acrosomal formation. Acid phosphatase activity was found in lysosomes of spermatogonia, spermatocytes, and spermatids, in lysosomes of Leydig cells, and in lysosomes, lipofuscin bodies, and Golgi cisternae of Sertoli cells. It is thought that Sertoli lysosomes play a role in the phagocytosis of degenerating germ cells; however, the role of spermatogenic or Leydig lysosomes is unknown. Adenosine triphosphatase activity occurred at the interfaces between two spermatogonia, and between Sertoli cells and spermatogonia, but was not observed in the spaces between two Sertoli cells, two spermatocytes, two spermatids, or between Sertoli cells and spermatocytes, or between Sertoli cells and spermatids.
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  • 5
    ISSN: 1432-0878
    Keywords: Chloride cell ; Gill filaments ; Ultrastructure ; Ion-transport ; Macrophthalmia stage ; Lampetra fluviatilis (L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The interplatelet epithelium of the gills of freshwater and seawater adapted macrophthalmia stages of the river lamprey, Lampetra fluviatilis, was studied by electron microscopy. Three main cell types: basal, ion-excretory and superficial cells were identified and described. Ion-excretory cells were exposed to the environment in sea water but covered by a layer of superficial cells in fresh water. Electron-dense secretory granules in the apex of the cell were more abundant in fresh water. In fresh water, a few of the superficial cells contained numerous mitochondria and were identified as ion-uptake cells. In view of the scarcity of ion-uptake cells, the possibility that the ion-excretory cells are also responsible for ion-uptake in fresh water is discussed. Phagocytic removal of degenerating ion-excretory cells may be an important mechanism for the conservation of materials in a starving animal.
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  • 6
    ISSN: 1432-0878
    Keywords: Chloride cell ; Gill filaments ; Ultrastructure ; Ion transport ; Migration ; River lamprey ; Lampetra fluviatilis (L)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two types of mitochondria-rich cells were found in the interplatelet areas of the gills of the migrating river lamprey. Both cell types are thought to be responsible for ion-transport across the gills. In the fresh-run migrant the gills are dominated by large, flask-shaped cells which show some ultra-structural similarities with the teleost chloride cell and have been tentatively referred to as ion-excretory cells. During the spawning migration the ion-excretory cells are replaced by smaller, mitochondria-rich cells which are similar in structure to the presumed ion-transporting cells in the ammocoete gill. They lack the tubular, smooth-membraned endoplasmic reticulum so characteristic of the lamprey ion-excretory cell and the teleost chloride cell and have been referred to as ion-uptake cells. The ion-uptake cells are found during the stenohaline, freshwater phases of the lamprey's life history. Ion-excretory cells are present during the periods of the life cycle when the animal is euryhaline. The possibility that the ion-excretory cells are also responsible for ion-uptake in fresh water is discussed.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 166 (1976), S. 275-283 
    ISSN: 1432-0878
    Keywords: Neurotrophic effects ; Smooth muscle ; Ultrastructure ; Aneurogenic amphibian larvae ; Smooth muscle diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural investigations showed that development and maintenance of smooth muscle was similar in control and aneurogenic amphibian larvae. This applies to both multi-unit and unitary smooth muscles. The gut musculature displayed a regional variation in smooth muscle morphology and a variety of intermuscular appositions even under conditions of nervelessness.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 172 (1976), S. 133-144 
    ISSN: 1432-0878
    Keywords: Cornea ; Nerves ; Rat ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the corneal nerves of the rat was studied in tissue fixed by immersion in and by perfusion with glutaraldehyde-containing fixatives. Of the four types of axonal terminal identified in the nerves, those with the features of adrenergic and cholinergic terminals were confined to the nerves at the limbus and were concentrated in the perivascular plexuses. The remaining two types of terminal were found on axons located in all parts of the cornea and on both intraepithelial axons and axons in the stromal nerves. Of these, one contained the numerous mitochondria which occur in the terminals of axons associated with known mechanoreceptors and the second contained variable and often small numbers of both clear and large dense-cored vesicles. While most of the mitochondria-containing terminals were seen in nerves located near the periphery, vesicle-containing terminals were numerous in all of the nerves, and especially in those in the avascular cornea. In material fixed by immersion in glutaraldehyde-paraformaldehyde, the vesicle-containing terminals appeared to be dilated, but in material fixed by perfusion there was little evidence of any increase in the diameter of the axons in the terminal regions. The structure of the terminals was compared with that of the terminals of axons identified in the nerves of the skin and the urinary tract and the differences in the vesicle content of the terminals to those reported in other studies of the corneal nerves was related to the use of different fixation procedures. The possibility that axons possessing such terminals are identical with the beaded axons and both the cholinesterase-positive and fluorescent axons demonstrated in light microscopical studies of the corneal nerves is discussed, and the widespread distribution of the axons in the cornea is equated with the hypothesis that they are afferent in nature and represent the peripheral receptors for pain impulses.
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